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        The Influence of IgE on Cultured Human Mast Cells

        Pernille Munk Frandsen,Inge Jacoba Maria Kortekaas Krohn,Hans Jürgen Hoffmann,Peter Oluf Schiøtz 대한천식알레르기학회 2013 Allergy, Asthma & Immunology Research Vol.5 No.6

        Purpose: The mast cell plays a pivotal role in the human immune response. Crosslinking of 2 IgE molecules bound to the high affinity IgE receptor(FcεRI) on the surface of the mast cell results in mast cell degranulation and the release of several proinflammatory mediators. Patients with type-I allergyhave increased levels of IgE in the blood compared to healthy individuals. Methods: In a 6-week culture system of stem cells to human mastcells we investigated the effect of the concentration of IgE. The mast cells were cultured with different concentrations of IgE for the last 10 days ofthe maturation period. It was observed how the IgE concentration affects the histamine release, FcεRI density on the mast cell surface and the concentrationof other mediators. Results: A clear correlation between IgE concentration in culture medium and the release of histamine upon activationwas observed. It showed a bell-shaped dose response curve, with maximal response around an IgE-concentration of 250 ng/mL. Furthermore,the sensitivity of the mast cells and surface density of FcεRI on mast cell surface was also influenced by the IgE concentration in the culture medium. Conclusions: IgE in the culture medium during the last 10 days of mast cell maturation influences the release of the preformed mediator histamineafter mast cell activation and the density of FcεRI on the mast cell surface. The release of the de novo synthetized mediator prostaglandin D2and the expression of chymase and tryptase are not influenced by IgE in culture medium.

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