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A Phase 1 Study Using Autologous Natural Killer Cells in Patients with HAIC-Hepatocellular Carcinoma
( Sung Bum Cho ),( Chung Hwan Jun ),( Sung Kyu Choi ),( Woo Kyun Bae ),( Je Jung Lee ),( Yang Jun Kang ),( Cheol Kyun Cho ),( Yang Seok Ko ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1
Aims: Natural killer (NK) cell-based immunotherapy has recently been tried with advances of understanding the role of immune defense against hepatocellular carcinoma (HCC). To improve NK cells therapy, we focused to increasing delivery of NK cells and synergic effect combined with hepatic arterial infusion chemotherapy (HAIC). Methods: We did a prospective, open label, phase 1 trial of the safety and efficacy of autologous NK cells through hepatic arterial infusion as sequential therapy after HAIC in advanced HCC patients. Between March 2016 and July 2017, 11 patients were included who showed favorable response more than stable disease (SD) after 2 sessions of HAIC in advanced HCC patients with child A. A total 4 sessions of HAIC were performed the protocols of infusion of cisplatin (25/m2) and 5-fluorouracil (750/m<sup>2</sup>) for 4 days every 3-4 weeks interval. The peripheral blood mononuclear cells of patients by leukapheresis were ob-tained after 3<sup>rd</sup> HAIC and NK cells were expanded for 2 weeks under Current Good Manufacturing Practices (cGMP). Patients received planned dosage of NK cells through chemoport into hepatic artery for 5 days after 4<sup>th</sup> HAIC (3 patients; 2.5x108, 3 patients; 5x10<sup>8</sup>, 5 patients; injection of 10x10<sup>8</sup> NK cells). The primary end point was safety of NK cell injection; secondary endpoint included objective response rate (modified Response Evaluation Criteria In Solid Tumors), time to progression, dura-tion of response and immunologic efficacy. Results: Any adverse events of NK cells injection were none according to dosage. An objective response was observed in 7 patients (63.6%) included three complete responses and four partial responses. Stable disease was observed in 2 patients and progressive disease was in 2 patients and thus disease control rate was 81.8%. The mean duration of time to progression was 9.7±5.3 month and duration of response without chemotherapy was 6.1±5.2 month. The newly metastatic lesion was occurred in 3 patents (27.2%; lymph node 1 patients, Lung 2 patients). Two patients were died by tumor progression and others were still alive. The increasing immunologic response was observed in 5 patients (55 %) to evaluate cytotoxicity and NK cell proportion of peripheral mononuclear cells after NK cell injection. Conclusions: The HAIC and NK cells immunotherapy is safe and effective treatment in the advance HCC patient with favorable liver function. The additional studies are urgently required to establish the new novel treatment.
Meter-long coated conductor by R2R PVD methods on RABiTS template
Ko, Rock-Kil,Kim, Ho-Sup,Ha, Hong-Soo,Chung, Jun-Ki,Yang, Joo-Saing,Park, Yu-Mi,Shi, Dong-Qi,Song, Kyu-Jeong,Park, Chan,Yoo, Sang-Im,Moon, Seung-Hyun,Kim, Young-Cheol The Korea Institute of Applied Superconductivity a 2004 한국초전도저온공학회논문지 Vol.6 No.4
Three film deposition systems (pulsed laser deposition, sputtering, and evaporation) equipped with reel-to-reel metal tape moving apparatus were installed and used to make meter-long coated conductor. Buffer architecture of $CeO_2/YSZ/Y_2O_3$ was deposited on Ni alloy using sputtering, evaporation, and PLD. YBCO superconducting layer was continuously deposited on buffered metal tape by PLD. End-to-end critical current ($I_c$) of 107 A at 77 K, self-field has been achieved in 1 em-wide tape (thickness 0.6∼1.0${\mu}{\textrm}{m}$, tape moving speed 54∼72 cm/hr) over 1 meter length.
First Report of Fusarium Wilt Caused by Fusarium proliferatum on Safflower
Sang Gyu Kim,Ho-Cheol Ko,On-Sook Hur,Binod Prasad Luitel,Ju-Hee Rhee,Mun-Sup Yoon,Hyung-Jin Baek,Kyoung-Yul Ryu,Jung Sook Sung 한국식물병리학회 2016 식물병연구 Vol.22 No.2
Wilt disease appeared the first in greenhouse-grown safflower (Carthamus tinctorius) in Jeonju, Korea. With the advancement of the disease, the infected plants were withered and died. In order to investigate the causal organism of this symptom disease, fungus was isolated from the infected plants and cultured on potato dextrose agar medium. The fungus showed the white or orange colony color with aerial mycelium. Macroconidia were from falcate to straight, usually 3–5 septate with 38.0–66.7× 2.9–4.4 μm. The fungus was inoculated to a new safflower plant and caused the same wilt. With morphological characters and pathogenicity results, sequence analyses (internal transcribed spacer ribosomal DNA and translation elongation factor 1α) suggested that, the isolated fungus is Fusarium proliferatum. This is the first report of Fusarium wilt disease caused by F. proliferatum on safflower in Korea.
Improvement in Dimensional Accuracy of Roll-Die-Formed Clutch Hub Used in Automotive Transmission
Ko, Dae-Hoon,Lee, Sang-Kon,Kwon, Yong-Nam,Kim, Sang-Woo,Kim, Bong-Hwan,Kim, Byung-Min,Ko, Dae-Cheol 한국정밀공학회 2012 International Journal of Precision Engineering and Vol.13 No.2
Roll die forming is investigated as a new process for manufacturing a clutch hub; in this process a tooth shape is formed by a deep drawing process using a die set with rotational rolls. Some problems are still encountered during roll die forming, such as a proper process design that takes into consideration the forming limit of sheet material, the improvement in the wear resistance of the punch and the rotational roll, and the control of dimensional accuracy. Particularly, the dimensional accuracy of the final product is affected by the clearance between the punch and the roll. Therefore, the objective of this study is to determine the optimal clearance between the punch and the roll for improving the dimensional accuracy during the roll die forming for a clutch hub. FE-simulation and the design of experiment method are employed to determine the optimal clearance required to produce a final product with satisfactory dimensional specifications. An experiment is performed under the same process conditions as the FE-simulation with an optimal clearance to validate the results obtained in this study. The results of the FE-simulation are in good agreement with the experimental ones within a maximum error of 5.55[%].
Genomic profiling combined with gene expression profiling in primary central nervous system lymphoma
Sung, Chang Ohk,Kim, Sang Cheol,Karnan, Sivasundaram,Karube, Kennosuke,Shin, Hyung Jin,Nam, Do-Hyun,Suh, Yeon-Lim,Kim, Seok-Hyung,Kim, Ji Yeon,Kim, Seok Jin,Kim, Won Seog,Seto, Masao,Ko, Young-Hyeh American Society of Hematology 2011 Blood Vol.117 No.4
<B>Abstract</B><P>Of the genetic changes in primary central nervous system lymphoma (PCNSL), little is known. To detect copy number alterations and differentially expressed genes in PCNSL, we analyzed a total of 12 PCNSL samples with high-resolution array-based comparative genomic hybridization and performed expression profiling in 7 of the 12 samples. The most frequent deletion found in 8 patients (66.7%) occurred in 9p21.3 containing CDKN2A. We compiled the top 96 genes (family-wise error rate, P < .05) showing the greatest differential expression between PCNSL and normal lymph node tissues. From these, we selected 8 candidate genes (NPFFR2, C4orf7, OSMR, EMCN, TPO, FNDC1, COL12A1, and MSC) in which expression changes were associated with copy number aberrations. All 8 genes showed both down-regulation in expression microarray and deletion in array-based comparative genomic hybridization analyses. These genes participate in cell signaling or cell adhesion. In addition, low mRNA expression of C4orf7 was significantly associated with poor survival (P = .0425). Using gene set enrichment analysis, we identified several signal transduction pathways, such as Janus kinase-signal transducers and activators of transcription pathway and adhesion-related pathways, which may be involved in pathogenesis of PCNSL. In conclusion, this study identified novel tumor suppressor genes that may serve as therapeutic targets of PCNSL.</P>
Large Eddy Simulation of Turbulent Premixed Flame in Turbulent Channel Flow
Ko Sang-Cheol,Park Nam-Seob The Korean Society of Mechanical Engineers 2006 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.20 No.8
Large eddy simulation of turbulent premixed flame in turbulent channel flow is studied by using G-equation. A flamelet model for the premixed flame is combined with a dynamic subgrid combustion model for the filtered propagation flame speed. The objective of this work is to investigate the validity of the dynamic subgrid G-equation model to a complex turbulent premixed flame. The effect of model parameters of the dynamic sub grid G-equation on the turbulent flame speed is investigated. In order to consider quenching of laminar flames on the wall, wall-quenching damping function is employed in this calculation. In the present study, a constant density turbulent channel flow is used. The calculation results are evaluated by comparing with the DNS results of Bruneaux et al.
Simple Purification of Bromelain from Pineapple
Ko, Bo-Sung,Hwang, Yong-Il,Lee, Seung-Cheol The Korean Society of Food Science and Nutrition 1996 Preventive Nutrition and Food Science Vol.1 No.1
Bromelain(EC 3.4.22.4), the collective name for the proteolytic enzymes found in tissues of the plant family Bromeliaceae(pineapple), has been used as a tenderizing agent in food processing, and as an antiinflammatory agent in pharmaceuticals. In this paper, we describe the simple purification method of bromelain using Korean pineapple fruit. After removing contaminants at 30% saturation of ammonium sulfate, the supernatant obtained was treated again with ammonium sulfate to 80% saturation. Wit the above salt fractionation, partially purified bromelain could be obtained. To get highly purified bromelain, the previous 30% to 80% ammonium sulfate treated precipitate was dialyzed against 25mM sodium acetate buffer(pH 5.0) followed by passing through a CM- cellulose cation exchange column. Fruit bromelain was eluted as a major peak at 0.5~0.8M NaCI gradient. The present method is simpler with high wield than the traditional purification method-acetone treatment and several consecutive chromatographic processes.