http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Khalijah, A. (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
-
Phuah, Neoh Hun,Azmi, Mohamad Nurul,Awang, Khalijah,Nagoor, Noor Hasima Korean Society for Molecular and Cellular Biology 2017 Molecules and cells Vol.40 No.4
MicroRNAs (miRNAs) are short non-coding RNAs that regulate genes posttranscriptionally. Past studies have reported that miR-210 is up-regulated in many cancers including cervical cancer, and plays a pleiotropic role in carcinogenesis. However, its role in regulating response towards anti-cancer agents has not been fully elucidated. We have previously reported that the natural compound 1'S-1'-acetoxychavicol acetate (ACA) is able to induce cytotoxicity in various cancer cells including cervical cancer cells. Hence, this study aims to investigate the mechanistic role of miR-210 in regulating response towards ACA in cervical cancer cells. In the present study, we found that ACA down-regulated miR-210 expression in cervical cancer cells, and suppression of miR-210 expression enhanced sensitivity towards ACA by inhibiting cell proliferation and promoting apoptosis. Western blot analysis showed increased expression of mothers against decapentaplegic homolog 4 (SMAD4), which was predicted as a target of miR-210 by target prediction programs, following treatment with ACA. Luciferase reporter assay confirmed that miR-210 binds to sequences in 3'UTR of SMAD4. Furthermore, decreased in SMAD4 protein expression was observed when miR-210 was overexpressed. Conversely, SMAD4 protein expression increased when miR-210 expression was suppressed. Lastly, we demonstrated that overexpression of SMAD4 augmented the anti-proliferative and apoptosis-inducing effects of ACA. Taken together, our results demonstrated that down-regulation of miR-210 conferred sensitivity towards ACA in cervical cancer cells by targeting SMAD4. These findings suggest that combination of miRNAs and natural compounds could provide new strategies in treating cervical cancer.
-
Neoh Hun Phuah,Mohamad Nurul Azmi,Khalijah Awang,Noor Hasima Nagoor 한국분자세포생물학회 2017 Molecules and cells Vol.40 No.4
MicroRNAs (miRNAs) are short non-coding RNAs that regulate genes posttranscriptionally. Past studies have reported that miR-210 is up-regulated in many cancers including cervical cancer, and plays a pleiotropic role in carcinogenesis. However, its role in regulating response towards anti-cancer agents has not been fully elucidated. We have previously re-ported that the natural compound 1'S-1'-acetoxychavicol acetate (ACA) is able to induce cytotoxicity in various cancer cells including cervical cancer cells. Hence, this study aims to investigate the mechanistic role of miR-210 in regulating response towards ACA in cervical cancer cells. In the present study, we found that ACA down-regulated miR-210 expression in cervical cancer cells, and suppression of miR-210 expression enhanced sensitivity towards ACA by inhibiting cell proliferation and promoting apoptosis. Western blot analysis showed increased expression of mothers against decapentaplegic homolog 4 (SMAD4), which was predicted as a target of miR-210 by target prediction programs, following treatment with ACA. Luciferase reporter assay confirmed that miR-210 binds to sequences in 3UTR of SMAD4. Furthermore, decreased in SMAD4 protein expression was observed when miR-210 was overexpressed. Conversely, SMAD4 protein expression increased when miR-210 expression was suppressed. Lastly, we demonstrated that overexpression of SMAD4 augmented the anti-proliferative and apoptosis-inducing effects of ACA. Taken together, our results demonstrated that down-regulation of miR-210 conferred sensitivity towards ACA in cervical cancer cells by targeting SMAD4. These findings suggest that combination of miRNAs and natural compounds could provide new strategies in treating cervical cancer.
-
Baki, B. B.,Remy, M. O.,Aini, H.,Khalijah, A.,Fujii, Y.,Annuar, M. S. M.,Zazali, A. 韓國雜草學會 2009 Weed&Turfgrass Science Vol.29 No.3
Dodder (Cuscuta campestris Yuncker) is a problematic weed in abandoned, derelict, open crop areas in Malaysia. Surveys were conducted in 2008-2009 in the state of Johore, Peninsular Malaysia to assess the extent of spread and distribution of C. campestris, and enlist its host range and damage susceptibility among crop plants and weed species. No less than 12 crop plants and 70 weed species were hosts to C. campestris in about 45,500 ha of crop and non-crop land surveyed in Johore, Peninsular Malaysia. Asystasia gangetica, Mikania micrantha, and Chromolaena odorata were the most common hosts among weed species while dodder was prevalent among cover crops (Calopogonium mucunoides and Pueraria phaseoloides), young tapioca, oil palm, and rubber plants. There were site- and host-mediated differences in the extent of spatio-temporal spread of dodder throughout the areas surveyed, although no significant differences were registered ob the extent of spread in the three consecutive surveys at 4-monthly intervals. The areas of spread range from about 0.36 ㎡ to 250,000 ㎡. The dodder populations displayed highly clumped spatial distribution pattern with variance-mean-ratio values ranging from 38.17 for Segamat populations to 123776.56 for Kota Tinggi equivalents. The parallel figures of Lloyd’s patchiness index were 1.19 and 1.38 Leaf disc of weed crop plants exposed to 1% or more of ethanol extracts of dodder indicated that A, gangetica, Ageratum conyzoides, Cassia alata, M. micrantha, Murdannia nudiflora, Phyllantus niruri, P. urinaria and Saccharum multiflorum were the most susceptible weed species, while Manihot esculenta was among the moderately susceptible crop plant.
-
New Azafluorenone Derivative and Antibacterial Activities of Alphonsea cylindrica Barks
Munirah Abdul Talip,Saripah Salbiah Syed Abdul Azziz,Chee Fah Wong,Khalijah Awang,Humera Naz,Yuhanis Mhd Bakri,Mohamad Syahrizal Ahmad,Marc Litaudon 한국생약학회 2017 Natural Product Sciences Vol.23 No.3
A phytochemical study of Alphonsea cylindrica King (unreported) has led to the isolation of six alkaloids. The compounds were identified as kinabaline (1; azafluorenone alkaloid), muniranine (2), O-methylmoschatoline (3; oxoaporphine alkaloid), lysicamine (4), atherospermidine (5) and N-methylouregidione (6; 4, 5-dioxoaporphine alkaloid). The structures of the isolated compounds were determined based on the spectroscopic techniques and by comparison with data reported in the literature. Alkaloid 2 was isolated as a new derivative of azafluorenone while alkaloids 1, 3 - 6 were isolated for the first time from Alphonsea species. In addition, alkaloid 3 and 4 showed inhibition zone against Staphylococcus aureus, Pseudomonas aeruginosa and Bacillus cereus in disc diffusion test. The minimum inhibition concentration (MIC) values of lysicamine (4) against S. aureus, B. cereus and P. aeruginosa were found to be smaller than O-methylmoschatoline (3). Therefore, the reported antibacterial activity showed the potential of this plant as natural antibacterial agent and supported the documented traditional use of Alphonsea sp. in the treatment of diarrhea and fever.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
