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Qiong Wu,Linquan Bai,Weicheng Liu,Yingying Li,Caige Lu,Yaqian Li,Kehe Fu,Chuanjin Yu,Jie Chen 한국미생물학회 2013 The journal of microbiology Vol.51 No.2
Streptomyces lydicus A01 and Trichoderma harzianum P1are potential biocontrol agents of fungal diseases in plants. S. lydicus A01 produces natamycin to bind the ergosterol of the fungal cell membrane and inhibits the growth of Botrytis cinerea. T. harzianum P1, on the other hand, features high chitinase activity and decomposes the chitin in the cell wall of B. cinerea. To obtain the synergistic biocontrol effects of chitinase and natamycin on Botrytis cinerea, this study transformed the chit42 gene from T. harzianum P1 to S. lydicus A01. The conjugal transformant (CT) of S. lydicus A01 with the chit42 gene was detected using polymerase chain reaction (PCR). Associated chitinase activity and natamycin production were examined using the 3, 5-dinitrosalicylic acid (DNS)method and ultraviolet spectrophotometry, respectively. The S. lydicus A01-chit42 CT showed substantially higher chitinase activity and natamycin production than its wild type strain (WT). Consequently, the biocontrol effects of S. lydicus A01-chit42 CT on B. cinerea, including inhibition to spore germination and mycelial growth, were highly improved compared with those of the WT. Our research indicates that the biocontrol effect of Streptomyces can be highly improved by transforming the exogenous resistance gene, i.e. chit42from Trichoderma, which not only enhances the production of antibiotics, but also provides a supplementary function by degrading the cell walls of the pathogens.
Production of drinking water from saline water by direct contact membrane distillation (DCMD)
Ke He,문일식,Ho Jung Hwang,Myung Wu Woo 한국공업화학회 2011 Journal of Industrial and Engineering Chemistry Vol.17 No.1
Nine types of commercially available membranes were used for a DCMD system to investigate the effect of membrane difference on permeation flux and conductivity. Some characteristics such as liquid entry pressure (LEP), contact angle (CA), gas permeability were checked to understand the membranes more comprehensively. Three different depth membrane modules were set up to study the heat and mass transfer process in a DCMD system. The effect of different operation parameters were studied including flow mode, flow rate, temperature, and NaCl concentration. The permeation conductivity values were under 8 mS/cm for all the experimental conditions. With real seawater, the permeate flux dropped from 23.76 L/m2 h to 14.36 L/m2 h over one month at the following conditions: hot side inlet temperature of 60 8C, cold side inlet temperature of 20 8C, and hot and cold side flow rate of 0.6 L/min for PTFE pore size 0.22 mm membranes. After cleaning, the membrane successfully reused in the DCMD system. A mathematical model was built to simulate the mass and heat transfer process, and the experimental results agree with the theoretical calculations.