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Experimental Study on Dynamic Water Grouting of Modified Water-Soluble Polyurethane
Xiaofan Liu,Junguang Wang,Kun Huang,Fengyun Li 대한토목학회 2019 KSCE Journal of Civil Engineering Vol.23 No.9
In underground project, water inrush disaster often occurs, resulting in a large number of casualties and economic losses. To solve these problems, grouting is one of the main techniques for controlling water inrush. At present, the research results on the treatment of water inrush by grouting are based on anhydrous or hydrostatic grouting. However, the study of dynamic water grouting is relatively few and the grouting materials are a little bit. In this paper, water-soluble polyurethane was selected as grouting material, modified by adding hydroxypropyl methyl cellulose, and the bond strength and microstructure change before and after modification are studied via bond strength experiment and microscopic observation. In addition, the WPU (water-soluble polyurethane) diffusion regularity of dynamic water grouting is studied by indoor flat grouting test. The research also adopts the Bingham fluid model according to the slurry characteristics to derive the grouting diffusion radius. The results show that the compactness of HPMC (hydroxypropyl methyl cellulose)-WPU is improved, the heterogeneity is reduced by 50.4%, and the bonding strength is increased by 153%. Therefore, the anti-scour ability of the HPMC-WPU is enhanced. The deviation of the WPU in the X-axis diffusion radius is 7.7 cm, and the HPMC-WPU is 4.39 cm. What’s more, the formula of grouting diffusion radius is derived. By comparing the formula with experiment results, the deviation is less than 15%, therefore, the formula has the significance of guiding engineering practice.
Atomic Force Microscopy of Asymmetric Membranes from Turtle Erythrocytes
Tian, Yongmei,Cai, Mingjun,Xu, Haijiao,Ding, Bohua,Hao, Xian,Jiang, Junguang,Sun, Yingchun,Wang, Hongda Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.8
The cell membrane provides critical cellular functions that rely on its elaborate structure and organization. The structure of turtle membranes is an important part of an ongoing study of erythrocyte membranes. Using a combination of atomic force microscopy and single-molecule force spectroscopy, we characterized the turtle erythrocyte membrane structure with molecular resolution in a quasi-native state. High-resolution images both leaflets of turtle erythrocyte membranes revealed a smooth outer membrane leaflet and a protein covered inner membrane leaflet. This asymmetry was verified by single-molecule force spectroscopy, which detects numerous exposed amino groups of membrane proteins in the inner membrane leaflet but much fewer in the outer leaflet. The asymmetric membrane structure of turtle erythrocytes is consistent with the semi-mosaic model of human, chicken and fish erythrocyte membrane structure, making the semi-mosaic model more widely applicable. From the perspective of biological evolution, this result may support the universality of the semi-mosaic model.
Atomic Force Microscopy of Asymmetric Membranes from Turtle Erythrocytes
Yongmei Tian,Mingjun Cai,Haijiao Xu,Bohua Ding,Xian Hao,Junguang Jiang,Yingchun Sun,Hongda Wang 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.8
The cell membrane provides critical cellular functions that rely on its elaborate structure and organization. The structure of turtle membranes is an important part of an ongoing study of erythrocyte membranes. Using a combination of atomic force microscopy and single-molecule force spectroscopy, we characterized the turtle erythrocyte membrane structure with molecular resolution in a quasi-native state. High-resolution images both leaflets of turtle erythrocyte membranes revealed a smooth outer membrane leaflet and a protein covered inner membrane leaflet. This asymmetry was verified by single-molecule force spectroscopy, which detects numerous exposed amino groups of membrane proteins in the inner membrane leaflet but much fewer in the outer leaflet. The asymmetric membrane structure of turtle erythrocytes is consistent with the semi-mosaic model of human, chicken and fish erythrocyte membrane structure, making the semi-mosaic model more widely applicable. From the perspective of biological evolution, this result may support the universality of the semi-mosaic model.