http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Jung, Joo Eun,Kim, Hong Sook,Lee, Chang Seok,Park, Dae-Hun,Kim, Yong-Nyun,Lee, Min-Jae,Lee, Jung Weon,Park, Jong-Wan,Kim, Myung-Suk,Ye, Sang Kyu,Chung, Myung-Hee Oxford University Press 2007 Carcinogenesis Vol.28 No.8
<P>Tumor angiogenesis is required for tumor development and is stimulated by angiogenic inducers like VEGF (vascular endothelial growth factor). Our previous study demonstrated that STAT3 (signal transducer and activator of transcription 3) up-regulates HIF-1alpha (hypoxia inducible factor-1alpha) protein stability and enhances HIF-1-mediated VEGF expression in hypoxic solid tumor cells, thus suggesting that the inhibition of STAT3 signaling may have clinical applications. In this study, we examined in vitro and in vivo, whether caffeic acid (CA) or its derivative CADPE [3-(3,4-dihydroxy-phenyl)-acrylic acid 2-(3,4-dihydroxy-phenyl)-ethyl ester] exert anticancer activity by targeting STAT3. It was found that CA or CADPE significantly inhibit STAT3 activity, and that this in turn down-regulates HIF-1alpha activity. Consequently, sequential blockade of STAT3 and HIF-1alpha resulted in the down-regulation of VEGF by inhibiting their recruitment to the VEGF promoter. In mice bearing a Caki-I carcinoma, both CA and CADPE retarded tumor growth and suppressed STAT3 phosphorylation, HIF-1alpha expression, vascularization and STAT3-inducible VEGF gene expression in tumors. Taken together, our results demonstrate that CA and CADPE are potential inhibitors of STAT3 and that they suppress tumor angiogenesis by inhibiting the activity of STAT3, the expression of HIF-1alpha and VEGF.</P>
Sang Hyun Shin,Jung Hun Pak,Mi Jin Kim,Hye Jeong Kim,Jai Heon Lee,Doh Hoon Kim,Hong Kyu Choi,Kyung Ho Kang,Ji Ung Jeong,Chon Silk Kang,Ho Won Jung,Young Soo Chung 한국육종학회 2012 한국육종학회지 Vol.44 No.1
Wild rice might have previously unidentified genes important for disease resistance and stress tolerance in response to biotic and abiotic stresses. A set of subtractive library was constructed both from leaves of wild rice plants, Oryza grandiglumis (CCDD, 2n=48), treated with fungal elicitor and from wounded leaves. A partial fragment that was homologous to PR10 genes from other plant species was identified via suppression subtractive hybridization and cDNA macroarray. The obtained full-length cDNA sequence (OgPR10) contains an open reading frame of 480 bp nucleotide, encoding 160 amino acids with a predicted molecular mass of 16.944 kDa and an isoelectric point (pI) of 4.91. The multiple alignment analyses showed the higher sequence homology of OgPR10 with PR10 genes identified in rice plants at amino acid level. The OgPR10 mRNA was not expressed by treatment with wounding, jasmonic acid, and salicylic acid, but markedly expressed in leaves treated with protein phosphatase inhibitors cantharidin and endothall, and yeast extract. In addition, the expression of OgPR10 mRNA was induced within 72 h after treatment with probenazole, one of well-known chemical elicitors, and reached the highest level at 144 h. Heterologous expression of OgPR10 caused growth inhibition and seedling lethality in E. coli and Arabidopsis, respectively. Chemically induced OgPR10 expression with glucocorticoid-mediated transcriptional induction system further reconfirmed its lethality on Arabidopsis seedling. In addition, OgPR10-expressing rice plants, Oryzae sativar were resistant against the infection of rice blast fungus, Magnaporthe grisea. These results indicate that OgPR10 is involved in probenazole- and microbe associated molecular patterns-mediated disease resistance responses in plants and is a potential gene for developing disease resistance crop plants.
Effect of Temperature on Survival of Escherichia coli O157
Kyu-Seok Jung,Sung-Gi Heu,Eun-Jung Roh,Min-Ha Kim,Hyun-Ji Gil,Na-Young Choi,Dong-Hwan Lee,Jeong-A Lim,Jae-Gee Ryu,Kye-Hoon Kim 한국토양비료학회 2013 한국토양비료학회지 Vol.46 No.6
Animal manure compost is a commonly used fertilizer in organic vegetable and fruit production in Korea. However, livestock manure compost produced from animal feces can contain a lot of the non-pathogenic and pathogenic bacteria. Of particular concern are bacteria causing human food-borne illness such as Escherichia coli O157:H7 and Listeria monocytogenes. The objective of this study was to investigate effect of temperature on survival of E. coli O157:H7 and L. monocytogenes in livestock manure compost. Commercial livestock manure compost (manure 60%, sawdust 40%) was inoculated with E. coli O157:H7 and L. monocytogenes. Compost was incubated at four different temperatures (10, 25, 35, and 55℃) for 20 weeks. Samples were taken every week during incubation depending on the given conditions. E. coli O157:H7 persisted for up to 1 day in livestock manure compost at 55℃, over 140 days at 10℃, 140 days at 25℃, and 120 days at 35℃, respectively. L. monocytogenes persisted for up to 1 day in livestock manure compost at 55℃ and 140 days at 10℃, 70 days at 25℃, and 40 days at 35℃, respectively. The results indicated that E. coli O157:H7 and L. monocytogenes persisted longer under low temperature condition. E. coli O157:H7 survived longer than L. monocytogenes at three different temperatures (10, 25, and 35℃). The results are being used to develop guidelines on the management of manure to reduce the risks of E. coli O157:H7 and L. monocytogenes transmission to foods produced in the presence of animal waste.
Jung, Kwang Hwa,Kim, Jeong Kyu,Noh, Ji Heon,Eun, Jung Woo,Bae, Hyun Jin,Xie, Hong Jian,Ahn, Young Min,Park, Won Sang,Lee, Jung Young,Nam, Suk Woo Wiley Subscription Services, Inc., A Wiley Company 2010 Journal of cellular biochemistry Vol.110 No.3
<P>The Wnt/β-catenin signaling pathway regulates various aspects of development and plays important role in human carcinogenesis. Nemo-like kinase (NLK), which is mediator of Wnt/β-catenin signaling pathway, phosphorylates T-cell factor/lymphoid enhancer factor (TCF/LEF) factor and inhibits interaction of β-catenin/TCF complex. Although, NLK is known to be a tumor suppressor in Wnt/β-catenin signaling pathway of colon cancer, the other events occurring downstream of NLK pathways in other types of cancer remain unclear. In the present study, we identified that expression of NLK was significantly up-regulated in the HCCs compared to corresponding normal tissues in five selected tissue samples. Immunohistochemical analysis showed significant over-expression of NLK in the HCCs. Targeted-disruption of NLK suppressed cell growth and arrested cell cycle transition. Suppression of NLK elicited anti-mitogenic properties of the Hep3B cells by simultaneous inhibition of cyclinD1 and CDK2. The results of this study suggest that NLK is aberrantly regulated in HCC, which might contribute to the mitogenic potential of tumor cells during the initiation and progression of hepatocellular carcinoma; this process appears to involve the induction of CDK2 and cyclin D1 and might provide a novel target for therapeutic intervention in patients with liver cancer. J. Cell. Biochem. 110: 687–696, 2010. © 2010 Wiley-Liss, Inc.</P>
Jung, Keun-Hwa,Chu, Kon,Lee, Soon-Tae,Kim, Juhyun,Sinn, Dong-In,Kim, Jeong-Min,Park, Dong-Kyu,Lee, Jung-Ju,Kim, Seung U.,Kim, Manho,Lee, Sang Kun,Roh, Jae-Kyu Elsevier 2006 Neurobiology of disease Vol.23 No.2
<P><B>Abstract</B></P><P>Recent evidences suggest key roles of abnormal neurogenesis and astrogliosis in the pathogenesis of epilepsy. Alterations in the microenvironment of the stem cell, such as microglial activation and cyclooxygenase-2 induction may cause ectopic neurogenesis or astrogliosis. Here, we examined if inflammatory blockade with celecoxib, a selective cyclooxygenase-2 inhibitor, could modulate the altered microenvironment in the epileptic rat brain. Celecoxib attenuated the likelihood of developing spontaneous recurrent seizures after pilocarpine-induced prolonged seizure. During the latent period, celecoxib prevented neuronal death and microglia activation in the hilus and CA1 and inhibited the generation of ectopic granule cells in the hilus and new glia in CA1. The direct inhibition of precursor cells by celecoxib was further demonstrated in human neural stem cells culture. These findings raise the evidence of COX-2 induction to act importantly on epileptogenesis and suggest a potential therapeutic role for COX-2 inhibitors in chronic epilepsy.</P>
Jung, Sung-Chul,Park, Eun-Sook,Choi, Eun-Nam,Kim, Chi-Hwa,Kim, Su-Jin,Jin, Dong-Kyu Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.30 No.1
Mucopolysaccharidosis type II (MPS II; Hunter syndrome) is an X-linked inherited disorder caused by a deficiency of the enzyme iduronate-2-sulfatase (IDS), which results in the lysosomal accumulation of glycosaminoglycans (GAG) such as dermatan and heparan sulfate. Here, we report the generation of IDS knockout mice, a model of human MPS II, and an analysis of the resulting phenotype. We also evaluated the effect of gene therapy with a pseudotyped, recombinant adeno-associated virus 2/8 vector encoding the human IDS gene (rAAV-hIDS) in IDS-deficient mice. IDS activity and GAG levels were measured in serum and tissues after therapy. Gene therapy completely restored IDS activity in plasma and tissue of the knockout mice. The rescued enzymatic activity completely cleared the accumulated GAGs in all the tissues analyzed. This model can be used to explore the therapeutic potential of IDS replacement and other strategies for the treatment of MPS II. Additionally, AAV2/8 vectors have promising future clinical applications for the treatment of patients with MPS II.
White electroluminescence from a single polyfluorene containing bis-DCM units
Kyu Lee, Sang,Jung, Byung-Jun,Ahn, Taek,Kwan Jung, Young,Lee, Jeong-Ik,Kang, In-Nam,Lee, Jonghee,Park, Jong-Hwa,Ku Shim, Hong Wiley Subscription Services, Inc., A Wiley Company 2007 Journal of polymer science Part A, Polymer chemist Vol.45 No.15
<P>A series of fluorene-based copolymers composed of blue- and orange-light-emitting comonomers were synthesized through palladium-catalyzed Suzuki coupling reactions. 9,9-Dihexylfluorene and 2-(2,6-bis-{2-[1-(9,9-dihexyl-9H-fluoren-2-yl)-1,2,3,4-tetrahydroquinolin-6-yl]-vinyl}-pyran-4-ylidene)-malononitrile (DCMF) were used as the blue- and orange-light-emitting chromophores, respectively. The resulting single polymers exhibited simultaneous blue (423/450 nm) and orange (580–600 nm) emissions from these two chromophores. By adjusting the fluorene and DCMF contents, white light emission could be obtained from a single polymer; a device with an ITO/PEDOT:PSS/polymer/Ca/Al configuration was found to exhibit pure white electroluminescence with Commission Internationale de L'Eclairage (CIE) coordinates of (0.33, 0.31), a maximum brightness of 1180 cd/m<SUP>2</SUP>, and a current efficiency of 0.60 cd/A. Furthermore, the white light emission of this device was found to be very stable with respect to variation of the driving voltage. The CIE coordinates of the device were (0.32, 0.29), (0.32, 0.29), and (0.33, 0.31) for driving voltages of 7, 8, and 10 V, respectively. © 2007 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 45: 3380–3390, 2007</P> <B>Graphic Abstract</B> <P>The EL device based on the single polyfluorene chain containing bis-DCM units exhibited a pure white light emission with CIE coordinates of (0.33, 0.31) and a quite stable white-light emission at different driving voltages. <img src='wiley_img/0887624X-2007-45-15-POLA22090-gra001.gif' alt='wiley_img/0887624X-2007-45-15-POLA22090-gra001'> </P>