肉用種鷄의 育種開發을 위한 交雜試驗 : I. Selection of Superior Stock in Female Line Ⅰ.母系統 優良 交配 組合 選拔試驗

吳鳳國,呂政秀 서울大學校 農科大學 1977 서울대농학연구지 Vol.2 No.1

A Study on crossbreeding of Broiler Production in Poultry This study was carried out to improve broiler production and to estimate a genetic value and to select superior broiler breeder stock by means of crossbreeding under domestic feeding and management conditions. 1. Selection of superior stock in females. The data used in this study were the rocords of two selected superior breeder stocks, one strain of domestic superior broiler parent stock and added one strain of imported parent stock. A total of 1,020 chicks of parent stock were used in this experiment. The results obtained in this study are summarzed as follows; (1) The average viability of brooding period was 95.20% and the highest viability was A strain (98.85%), and lowest viability was D strain. There was significant difference between A and D strains. The death of the chicks was due mainly to paralysis and catarrh of crop. (2) The average viability of growing period was no singificant difference between experimental strains. (3) With respect to the laying hen viability, the average viability was 92.71%and the highest viabllity was 96.55% in C strain, and the lowest viability were 91% in A and D strains. The death of the laying hens was due mainly to Lymphoid Leukosis Complex in D strain, (4) The average body weight at 6 weeks of age was 953g and the highest body weight was 986g in D strain, and the lowest body weight was C strain (936g). The D strain was superior to the other strains in the body weight at 6 weeks of age and there was significant difference between D and others strains. (5) The average feed conversion at 6 weeks of age was 2.11kg and the highest feed effeciency was A strain (2.06kg). The A strain was improved feed efficiency about 0.1kg compared with D strains. (6) The growth rate of growing period was normally developed comparing with breeder pullet mean body weight and feed recommendations. There was no significant difference between experimental strains. (7) The average body weight at 24weeks of age was 2,775g and this body weight was slightly overweighed compared with recommended feeding standard, however there was no signifiicant difference between strains. (8) The average adult body weight at 58 weeks of age was 3,356g and highest body weight was 3,416g in D strain, and the lowest body weight was 3,296g in A strain. The adult body weight of laying period was normally maintained compared with breeder pullet feeding recommend-ations. (9) The average date of sexual maturity was 179days and the earlist sexual maturity was 176days in D strain and the latest sexual maturity was 183 days in C strain. (10) The average hen-day egg production from 24 to 58 weeks of age was 62% and the high-est egg production strain was A strain (66%), and the lowest egg production was D strain (60%). The A strain was about 6% higher than the imported parent stock (D strain). The hen-housed average egg production was 145 eggs and the A strain had about 11 egg higher egg production than the D strain. In statistical analysis for egg production, there was significant difference between A strain and the other strains. (11) In egg weight, the average egg weight was 61g and the highest egg weight was D strain-(62.7g) and the lowest egg weight strain was A strain (60g). It seems to be important to improve egg weight in domestic strains. (12) The average conversion of feed into egg production was 219.8g and the lowest feed requirement strain was A strain(206.6g), and the highest feed requirement strain was C strain(227.3g). It seems to be important in economic point and A strain was superior to the imported parent stock (D strain). (13) In economic analysis of broiler breeder stock, the average net income per layer was 2,661 won and the highest net income was A strain (2,919won) and the lowest net income was imported parent stock (2,402won). With respect to the cost of per egg production, the average egg production cost was 29 won and the lowest production cost was A strain(27.52won). Egg production cost of D strain which was imported broiler breeder stick was 30.29won and the D strain needed more cost 2.77won to produce per egg compared with the A strain. (14) Average fertility of selected superior stock in female lihe was 98.3% and the lowest fertility was B strain (97.9%). The average of hatchability was 93.8% and the highest hatchability was (B ×Y) crossbreds (96.3%) and the lowest hatchability was(B ×X) crossbred(93.6%) and imported parent stock (90.2%).

Bootstrap Analysis and Major DNA Markers of BM4311 Microsatellite Locus in Hanwoo Chromosome 6

Yeo, Jung-Sou,Kim, Jae-Woo,Shin, Hyo-Sub,Lee, Jea-Young Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.8

LOD scores related to marbling scores and permutation test have been applied for the purpose detecting quantitative trait loci (QTL) and we selected a considerable major locus BM4311. K-means clustering, for the major DNA marker mining of BM4311 microsatellite loci in Hanwoo chromosome 6, has been tried and five traits are divided by three cluster groups. Then, the three cluster groups are classified according to six DNA markers. Finally, bootstrap test method to calculate confidence intervals, using resampling method, has been adapted in order to find major DNA markers. It could be concluded that the major markers of BM4311 locus in Hanwoo chromosome 6 were DNA marker 100 and 95 bp.

Identification of Genetic Markers for Korean Native Cattle (Hanwoo) by RAPD Analysis

Yeo Jung Sou,Lee Ji Sun,Lee Chang Hee,Jung Young Ja,Nam Doo Hyun The Korean Society for Biotechnology and Bioengine 2000 Biotechnology and Bioprocess Engineering Vol.5 No.1

In order to develop the specific genetic marker for Korean native cattle (Hanwoo), randomly amplified polymorphic DNA (RAPD) analysis of 6 different cattle breeds was attempted by using 38 decamer primers. In comparison of RAPD patterns, two distinctive DNA bands specific for Hanwoo were detected. One was 296 bp of DNA fragment found to be specific only for female Hanwoo when primer GTCCACACGG was employed. In individual analysis of this RAPD marker was observed only in female individuals with the possibility of $85.3\%$. The other was 521 bp of RAPD marker amplified using TCGGCGATAG and AGCCAGCGAA primers, which showed $83.0\%$ of genetic frequency in 85 male and 68 female individuals tested. Nucleotide sequencing of these genetic markers revealed that 296 bp marker has a short micro satellite-like sequence, ACCACCACAC, and a tandem repeat sequence of microsatellite GAAAAATG in the determined sequence. Two distinctive tandem repeats of microsatellite sequences, MC and GAAGA, were also appeared in 521 bp DNA marker. In BLAST search, any gene having high homology with these markers was not found.

Hanwoo(Korean Cattle) Traceability Using DNA Markers

Yeo, Jung-Sou,Rhee, Sung-Won,Choi, Yu-Mi,Kwon, Jae-Chul,Lee, Jea-Young 한국통계학회 2006 Communications for statistical applications and me Vol.13 No.3

To apply and evaluate the effectiveness of genetic markers on Hanwoo traceability systems, samples of 33 Hanwoo individuals from Korean elite sire families were used, and five microsatellite markers were selected finally, which were located on chromosomes different chromosomes with the end sequencing of 100 HW-YUBAC that were recorded in the NCBI by Yeungnam University. Ten major microsatellite markers were selected from alleles amplified, their frequencies, H(Heterozygosity) and PIC(Polymorphism information content) with Hardy-Weinberg equilibrium. Next, in order to evaluate the power of the markers selected on the individual animal identification, the match probability(MP) and the relatedness coefficient(R) were computed.

Utilization of DNA Marker-Assisted Selection in Korean Native Animals

Yeo, Jung-Sou,Kim, Jae-Woo,Chang, Tae-Kyung,Park, Young-Ae,Nam, Doo-Hyun 영남대학교 약품개발연구소 2000 영남대학교 약품개발연구소 연구업적집 Vol.10 No.-

The recent progress of DNA technologies including DNA fingerprinting(DFP) and random amplified DNA polymorphism(RAPD) analysis make it possible to identify the spe-cific genetic traits of animals and to analyze the genetic diversity and relatedness between or within species or populations. Using those techniques, some efforts to identify and develop the specific DNA markers based on DNA polymorphism, which are related with economic traits for Korean native animals, Hanwoo(Korean native cattle), Korean native pig and Ko-rean native chichen, have been made in Korea for recent a few years. The developed specific DNA markers successfully characterize the Korean native animals as the unique Korean ge-netic sources, distinctively from other imported breeds. Some of these DNA markers have been related to some important economic traits for domestic animals, for example, growth rate and marbling for Hanwoo, growth rate and back fat thickness for native pig, and growth rate, egg weight and egg productivity for native chichen. This means those markers can be used in important marker-assisted selection(MAS) of Korean native domestic animals and further contribute to genetically improve and breed them.

닭(gallus domesticus)의 經濟形質 改良을 위한 染色體 分染分析과 標識因子의 究明에 관한 硏究 Ⅱ. 卵用種의 系統間의 比較

Jung Sou Yeo(呂政秀),Bong Kug Ohh(吳鳳國),Bong Bo Seo(徐奉甫) 한국육종학회 1985 한국육종학회지 Vol.17 No.1

This experiment was carried out to identify the differences of genetic materials as genetic marker for the four strains of egg type breed. The differences of strains of egg type chick were as follows. It was chracterized with strain A to show less band at distal part of chromosome #3 and dark band at centromere of #4-q. More bands at distal part of #1-q than others was the marker of strain K. In strain M, less band at #2-p and non-band at distal part of #Z-q were observed, and dark band at centromere of Z chromosome like the feature of meat type chick was the chracteristics of strain S.

Identification of Genetic Markers for Korean Native Cattle (Hanwoo) by RAPD Analysis

Yeo, Jung Sou,Lee, Ji Sun,Lee, Chang Hee,Jung, Young Ja,Nam, Doo Hyun 영남대학교 약품개발연구소 2000 영남대학교 약품개발연구소 연구업적집 Vol.10 No.-

In order to develop the specific genetic market for Korean native cattle(Honwoo), randomly amplified polymorphic DNA(RAPD) analysis of 6 different cattle breeds was at-tempted by using 38 decamer primers. In comparison of RAPD patterns, two distinctive DNA bands specific for Hanwoo were detected. One was 296 bp of DNA fragment found to be specific only for female Hanwoo when primer GTCCACACGG was employed. In individ-ual analysis of this RAPD marker was observed only in female individuals with the possibility of 85.3%. The other was 521 bp of RAPD marker amplified using TCGGCGATAG and AGCCAGCGAA primers, which showed 83.0% of genetic frequency in 85 male and 68 female individuals tested. Nucleotide sequencing of these genetic markers revealed that 296 bp marker has a short microsatellite-like sequence, ACCACCACAC, and a tandem repeat se-quence of microsatellite GAAAAATG in the determined sequence. Two distinctive tandem repeats of microsatellite sequences, AAC and GAAGA, were also appeared in 521 bp DNA marker. In BLAST search, any gene having high homology with these markers was not found

한우의 염색체 분염분석에 의한 유전적 조성의 특징에 관한 연구

여정수 ( Jung Sou Yeo ) 한국축산학회 1987 한국축산학회지 Vol.29 No.3

Chromosome analysis with the DNA structure is the new approach to identify the characteristics of genetic materials in the Korean Native Cattle. Chromosome number and morphology of the Korean Native Cattle were same as the aspects of other beef breeds reported already. G-banded chromosome pattern measured by densitometric measurement was similar to other reports for number and location of bands, but density of band was quite different. C-banding pattern, heterochromatic region, was estimated by the photo-electric recording and ratio of the band size per total chromosome. Dark bands were located at centromere of all chromosomes and especially banded regions of chromosome # 4, 19, 26, X and Y were above 40% of total size. Density of G-band and size of C-band in the Korean Native Cattle were considered the important tools in order to check the genetic marker related to performance.

난용계에 (卵用鷄) 있어서 상가적 (相加的) 및 모체유전 효과를 이용한 선발지수 추정

여정수(Jung Sou Yeo),오봉국(Bong Kug Ohh) 한국축산학회 1984 한국축산학회지 Vol.26 No.1

The data from the record of 1131 birds of synthetic population (A line) and 1587 birds of selected population (D line) through 3 generations (1977-1980) were used for the estimates of expected genetic gain by selection indices based on the additive effect only and maternal and additive effects. It was found through Hazel`s method considered the only additive effect that in both lines the first egg day and egg weight at 43 weeks of age were decreased while egg number to 43 weeks of age was increased, and body weight at 43 weeks of age was slightly decreased. And A line showed more satisfactory genetic changes compared with D line except body weight. Expected genetic gains in A line based on genetic parameters estimated using additive and maternal genetic effects simultaneously were the most significant for increase of egg number among the four traits studied and egg weight was 2.50g heavier than performance of this generation, while body weight and the first egg day were decreased, which were considered most desirable in genetic changes of layer. In line D genetic gain of egg production could be achieved through maternal genetic effect with maintaining body weight nearly constant. And major profitable egg weight was decreased by 0.87g lower than that of this generation, and the first egg day was also shortened. According to above results genetic improvement using the additive and maternal effects may be achieved more desirable than that using the additive effect only.

한국 재래축종인 (在來畜種) 한우의 염색체 분석

여정수 ( Jung Sou Yeo ) 한국축산학회 1984 한국축산학회지 Vol.26 No.3

For the purpose of the distinguishing the sophisticated chromosome spread procedure and chromosome banding pattern of the Korean Native Cattle, fifty heads were examined cytogenetically using the bone marrow culture technique. Red bone marrow from femur and sternum was mixed with one drop of 1% colchicine in 20 gage needle and 7-10 cc media, and then cultured for 2-3 hours at 37.5C. After hypotonic treatment by 1% sodium citrate for 30 minutes at 37.5C, it was fixed in 3:1 methanol acetic acid. The slides chromosome were prepared and air- dried. Metaphase plate of chromosome was found at slide treated by the Giemsa staining. Chromosome number of the Korean Native Cattle was 60 at haploid, as telocentric autosome and sex chromosome X and Y were sub-metacentric. To be clearly identify the homology chromosome and banding pattern in each chromosome complement, prepared chromosome slides were treated with 0.03-0.04% trypsin solution (with Hank`s BSS free Ca and Mg) at 25℃ for 10 minutes, and then rinsed 70% and 100% ethanol. In comparison standardised banding pattern of the Korean Native Cattle with the other cattle breeds, it was found that 7 pairs of autosome in the Korean Native Cattle; 6, 7, 16 19, 24, 25 and 27 were not matched with other domestic cattle.