Expression patterns of octoploid strawberry TGA genes reveal a potential role in response to Podosphaera aphanis infection

Jun Feng,Yang Cheng,Caixia Zheng 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.1

TGA family transcription factors are downstream components of the salicylic acid (SA) pathway and play a crucial role in the penetration defense against powdery mildew, a disease caused by the biotrophic pathogen Podosphaera aphanis that can cause enormous financial losses. However, the TGA genes in the octoploid strawberry (Benihoppe) remain largely unexplored. In this study, 11 FaTGA genes were identified and grouped into five subgroups based on the Strawberry GARDEN database. The evolution of TGAs has been relatively conservative, maintaining nearly the same protein domain and motif architectures. RT-qPCR showed that FaTGA s have different expression patterns in roots, stems, and leaves. The characteristics of their gene expression patterns indicate that FaTGA1, FaTGA2, FaTGA5, FaTGA7, FaTGA8, FaTGA9, and FaTGA10, as well as SA-responsive genes, were responsive to P. aphanis infection. In particular, exogenous SA induced significant upregulation of FaTGA and SA-responsive genes. Further correlation analysis suggested that FaTGA1, FaTGA2, FaTGA5, FaTGA7, FaTGA8, and FaTGA10 may play a crucial role in the response to powdery mildew disease. Taken together, our results reveal the responses of TGA genes in the strawberry P. aphanis interaction, which will provide further insight into the strawberry defense against powdery mildew.

Dynamic Analysis of Spine Sagittal Balance during Walking Using Motion Analysis Technology

Jun Feng,Yoon Hyuk Kim,Kyungsoo Kim 대한기계학회 2013 대한기계학회 춘추학술대회 Vol.2013 No.12

Clinical Effect of Traditional Chinese Medicine Shenhuang Granule in Critically Ill Patients with COVID-19: A Single-Centered, Retrospective, Observational Study

( Jun Feng ),( Bangjiang Fang ),( Daixing Zhou ),( Junshuai Wang ),( Dengxiu Zou ),( Gang Yu ),( Yikuan Fen ),( Dan Peng ),( Jifa Hu ),( Daqian Zhan ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.3

The coronavirus disease 2019 (COVID-19) pandemic has become a public health emergency of global concern. In China, traditional Chinese medicine has been widely administered to COVID-19 patients without sufficient evidence. To evaluate the efficacy of Shenhuang Granule (SHG) for treating critically ill patients with COVID-19, we included in this study 118 patients who were admitted to the ICU of Tongji Hospital between January 28, 2020 and March 28, 2020. Among these patients, 33 (27.9%) received standard care plus SHG (treatment group) and 85 (72.1%) received standard care alone (control group). Enrolled patients had a median (IQR) age of 68 (57-75) years, and most (79 [67.1%]) were men. At end point of this study, 83 (70.3%) had died in ICU, 29 (24.5%) had been discharged from ICU, and 6 patients (5.2%) were still in ICU. Compared with control group, mortality was significantly lower in treatment group (45.4% vs. 80%, p < .001). Patients in treatment group were less likely to develop acute respiratory distress syndrome (ARDS) (12 [36.3%] vs. 54 [63.5%], p = 0.012) and cardiac injury (5 [15.1%] vs. 32 [37.6%], p = 0.026), and less likely to receive mechanical ventilation (22 [66.7%] vs. 72 [84.7%], p = 0.028) than those in control group. The median time from ICU admission to discharge was shorter in treatment group (32 [20-73] days vs. 76 [63-79] days, p = 0.0074). These findings suggest that SHG treatment as a complementary therapy might be effective for critically ill adults with COVID-19 and warrant further clinical trials.

Simulation of the irradiation effect on hardness of Chinese HTGR A508-3 steels with CPFEM

Junfeng Nie,Pandong Lin,Yunpeng Liu,Haiquan Zhang,Xin Wang 한국원자력학회 2019 Nuclear Engineering and Technology Vol.51 No.8

Understanding the irradiation hardening effect of structural steels under various irradiation conditions plays an important role in developing advanced nuclear systems. Such being the case, a crystal plasticity model for body-centered cubic (BCC) crystal based on the density of dislocations and irradiation defects is summarized and numerically implemented in this paper. Based on this model, nano-indentation hardness of Chinese A508-3 steels with ion irradiation is calculated. Very good agreement is observed between simulation and experimental data of several different irradiation doses subjected to various operating temperatures, from which, it can be concluded that indentation hardness increases with increasing irradiation dose at both room temperature and high temperature. Consequently, the validity of this model has been proved properly, and furthermore, the model established in this paper could guide the study of irradiation hardening effect and temperature effect to some extent.

PSCArs2294008 T polymorphism increases the risk of bladder cancer in Bai, Dai, and Han ethnicity in China and a potential mechanism

Junfeng Yang,Wei Li,Zhuorui Zhang,Jie Shen,Ningnan Zhang,Min Yang,Maolin Yang,Yanhong Yu 한국유전학회 2018 Genes & Genomics Vol.40 No.5

The aim of this study is to make a comparative evaluation on association of PSCArs2294008 C/T polymorphism with the risk of bladder cancer in Bai, Dai, and Han people in China. A potential mechanism of the T allele risk was also investigated. T allele increased the occurring risk of bladder cancer in Han (OR 1.34; 95% CI 1.17–1.69), Dai, (OR 1.33; 95% CI 1.12–1.70), and Bai (OR 1.14; 95% CI 1.08–1.57) people. T genotype was also observed to associate with invasive bladder cancer in all the three populations (Bai, OR 1.15, 95% CI 1.07–1.87; Dai, OR 1.17, 95% CI 1.05–2.23; Han, OR 1.22, 95% CI 1.10–2.09). PSCA m-RNA levels in T genotype bladder cancer tissues were significantly lower than those in C genotype. An enhancement of PSCA m-RNA level by over-expressing C or T genotype in bladder cancer cells both decreased the cell proliferation and migration, but not affected cell cycle. The increased cell apoptasis due to the over-expression of the two variants was observed. Those change of cell proliferation, migration, and apoptasis was more remarkable in over-expressed C genotype cells than those in over-expressed T genotype. T genotype was genetically high risk to the occurrence of bladder cancer. The decreased PSCA m-RNA levels were involved in the progress of bladder cancer. T allele takes more responsibility for PSCA m-RNA down-regulation to promote cell proliferation and migration and hinder cell apoptasis, thus leading to a higher risk.

Detection of Bobbin Yarn Surface Defects by Visual Saliency Analysis

Junfeng Jing,Haiye Li,Huanhuan Zhang,Zebin Su,Kaibing Zhang 한국섬유공학회 2020 Fibers and polymers Vol.21 No.11

In order to solve the problem of unstable quality of the traditional bobbin yarn surface defect detection, a methodfor detecting the surface defect of bobbin yarn using visual saliency analysis is proposed. Firstly, the Difference of Gaussianalgorithm is used to suppress the texture of the image, and the contrast between the defect area and the background isenhanced. Then, the method of wavelet threshold is applied to filter the noise in the images after the Difference of Gaussian,and the detailed features of the defect area are preserved while the noise interference is eliminated. Finally, the frequencytuned visual saliency algorithm is used to extract and segment the defective regions accurately. After the process about theDifference of Gaussian and wavelet threshold denoising, the original image of the bobbin yarn can make the image highlightthe feature information of the defect area on the basis of ensuring elimination of the background texture and noise, andfacilitate the detection of the defect area by combining visual saliency analysis. In the detection of the accuracy and integrityof the defect area, the detection method of this paper is better than the control method, and the defect segmentation result ismore accurate. The proposed method for detecting the surface defect of the bobbin can not only completely eliminate theinfluence of the background texture of the bobbin surface, but also accurately and completely detect the surface defect of thebobbin, and the defect details remain intact. In this paper, the effectiveness of the algorithm is further verified by objectivetests. The precision and recall rate of the test are 95.15 % and 98.00 %, respectively, which can meet the needs of actualdetection.

Fast Result Enumeration for Keyword Queries on XML Data

Junfeng Zhou,Ziyang Chen,Xian Tang,Zhifeng Bao,TokWang Ling 한국정보과학회 2012 Journal of Computing Science and Engineering Vol.6 No.2

In this paper, we focus on efficient construction of tightest matched subtree (TMSubtree) results, for keyword queries on extensible markup language (XML) data, based on smallest lowest common ancestor (SLCA) semantics. Here, “matched” means that all nodes in a returned subtree satisfy the constraint that the set of distinct keywords of the subtree rooted at each node is not subsumed by that of any of its sibling nodes, while “tightest” means that no two subtrees rooted at two sibling nodes can contain the same set of keywords. Assume that d is the depth of a given TMSubtree, m is the number of keywords of a given query Q. We proved that if d ≤ m, a matched subtree result has at most 2m! nodes; otherwise, the size of a matched subtree result is bounded by (d ? m + 2)m!. Based on this theoretical result, we propose a pipelined algorithm to construct TMSubtree results without rescanning all node labels. Experiments verify the benefits of our algorithm in aiding keyword search over XML data.

Parameter optimization of five-axis polishing using abrasive belt flap wheel for blisk blade

Junfeng Zhang,Yaoyao Shi,Xiaojun Lin,Zhishan Li 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.10

Considering the weak rigidity of blisk blades and the limited accessibility of blisk tunnels, polishing blisk blades is difficult when using tools with poor flexibility and large dimension. Hence, to reduce the surface roughness (SR) of a blisk blade, five-axis polishing method with abrasive belt flap wheel (ABFW) is employed to polish the blisk blade based on the analysis of the blisk structure and advantages of ABFW polishing. Polishing experiments based on central composite design are performed using ABFW. Response surface method (RSM) is employed to establish a predictive model between SR and various parameters, including ABFW size, contact force, spindle speed, and feed rate. Analysis of variance is then performed to evaluate the proposed model. The degree of influence of each factor on SR after polishing using ABFW is determined by plotting main effects. The interactions of polishing factors on SR are analyzed by RSM. Optimal parameters are obtained by response surface optimization. Finally, an experiment on blisk blade polishing using ABFW on a five-axis polishing machine is carried out for confirmation. Results indicate that the surface quality of the blisk blade after polishing is significantly improved, with SR being less than 0.4 μm.

Efficient LDPC-Based, Threaded Layered Space-Time-Frequency System with Iterative Receiver

Junfeng Hu,Hailin Zhang,Yuan Yang 한국전자통신연구원 2008 ETRI Journal Vol.30 No.6

We present a low-density parity-check (LDPC)-based, threaded layered space-time-frequency system with emphasis on the iterative receiver design. First, the unbiased minimum mean-squared-error iterative-tree-search (U-MMSE-ITS) detector, which is known to be one of the most efficient multi-input multi-output (MIMO) detectors available, is improved by augmentation of the partial-length paths and by the addition of one-bit complement sequences. Compared with the U-MMSE-ITS detector, the improved detector provides better detection performance with lower complexity. Furthermore, the improved detector is robust to arbitrary MIMO channels and to any antenna configurations. Second, based on the structure of the iterative receiver, we present a low-complexity belief-propagation (BP) decoding algorithm for LDPC-codes. This BP decoder not only has low computing complexity but also converges very fast (5 iterations is sufficient). With the efficient receiver employing the improved detector and the low-complexity BP decoder, the proposed system is a promising solution to high-data-rate transmission over selective-fading channels.

Adaptive fast nonsingular terminal sliding mode control for underactuated surface vessel trajectory tracking

Junfeng Qin,Jialu Du 제어로봇시스템학회 2019 제어로봇시스템학회 국제학술대회 논문집 Vol.2019 No.10

In this paper, we investigate the trajectory tracking control problem for underactuated surface vessels (USVs) in the presence of time-varying disturbances with unknown upper bounds. First, in order to handle the underactuation of the USVs, we introduce a coordinate transformation to redefine the output variables of the USVs. Then, the adaptive trajectory tracking control law is proposed based on the adaptive fast nonsingular terminal sliding mode (FNTSM) control technique, where the adaptive law online provides the estimation of the parameters. It is proved that the designed control law can force the USV to track the desired trajectory and tracking errors of closed-loop system converge to zero in finite time. Simulation results on an underactuated model ship verify the effectiveness of the proposed control law.