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Park, Seong Bin,Hikima, Jun-ichi,Suzuki, Yoshiaki,Ohtani, Maki,Nho, Seong Won,Cha, In Seok,Jang, Ho Bin,Kondo, Hidehiro,Hirono, Ikuo,Aoki, Takashi,Jung, Tae Sung Elsevier 2012 DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Vol.36 No.4
<P><B>Highlights</B></P><P>► The gene encoding nucleotide-binding oligomerization domain 1 (NOD1) was cloned in olive flounder. ► NOD1 was expressed in all fish tissues examined. ► NOD1 mRNA levels was elevated in fish infected with <I>Edwardsiella tarda</I>, <I>Streptococcus iniae</I>, or VHSV. ► The inhibition of <I>E. tarda</I> and the increase of IL-1β levels were observed in NOD1 over-expressing cells.</P> <P><B>Abstract</B></P><P>The gene encoding nucleotide-binding oligomerization domain 1 (NOD1) was cloned from olive flounder (<I>Paralichthys olivaceus</I>) and the role played by NOD1 during <I>Edwardsiella tarda</I> infection was evaluated. The complete open reading frame of NOD1 was 2820bp in length, encoding a 939-amino acid polypeptide. The NOD1 protein contains three conserved domain structures including C-terminal LRRs, a central NACHT motif, and an N-terminal CARD domain, which show similarities of 49–74% to those of other vertebrate counterpart proteins. NOD1 expression was observed in all fish tissues examined, and the levels increased in olive flounder infected with <I>E. tarda</I>, <I>Streptococcus iniae</I>, or viral hemorrhagic septicemia virus (VHSV). When hirame natural embryo (HINAE) cells over-expressing NOD1 were infected with <I>E. tarda</I>, bacterial growth was inhibited, and the IL-1β transcript level increased compared to that of the control. These findings imply that NOD1 plays an important role in response to <I>E. tarda</I> infection of olive flounder.</P>
Cha Minseok,Kim Jun-Ha,Choi Hyo-Jin,Nho Soo Bin,Kim Soo-Yeon,Cha Young-Lok,Song Hyoungwoon,Lee Won-Heong,Kim Sun-Ki,Kim Soo-Jung 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.10
This work aimed to evaluate the feasibility of biohydrogen production from Barley Straw and Miscanthus. The primary obstacle in plant biomass decomposition is the recalcitrance of the biomass itself. Plant cell walls consist of cellulose, hemicellulose, and lignin, which make the plant robust to decomposition. However, the hyperthermophilic bacterium, Caldicellulosiruptor bescii, can efficiently utilize lignocellulosic feedstocks (Barley Straw and Miscanthus) for energy production, and C. bescii can now be metabolically engineered or isolated to produce more hydrogen and other biochemicals. In the present study, two strains, C. bescii JWCB001 (wild-type) and JWCB018 (ΔpyrFA Δldh ΔcbeI), were tested for their ability to increase hydrogen production from Barley Straw and Miscanthus. The JWCB018 resulted in a redirection of carbon and electron (carried by NADH) flow from lactate production to acetate and hydrogen production. JWCB018 produced ~54% and 63% more acetate and hydrogen from Barley Straw, respectively than its wild-type counterpart, JWCB001. Also, 25% more hydrogen from Miscanthus was obtained by the JWCB018 strain with 33% more acetate relative to JWCB001. It was supported that the engineered C. bescii, such as the JWCB018, can be a parental strain to get more hydrogen and other biochemicals from various biomass.
Comparative Sequence Analysis of a Multidrug-Resistant Plasmid from Aeromonas hydrophila
del Castillo, Carmelo S.,Hikima, Jun-ichi,Jang, Ho-Bin,Nho, Seong-Won,Jung, Tae-Sung,Wongtavatchai, Janenuj,Kondo, Hidehiro,Hirono, Ikuo,Takeyama, Haruko,Aoki, Takashi American Society for Microbiology 2013 Antimicrobial agents and chemotherapy Vol.57 No.1
<B>ABSTRACT</B><P>Aeromonas hydrophilais a pathogenic bacterium that has been implicated in fish, animal, and human disease. Recently, a multidrug resistance (MDR) plasmid, pR148, was isolated fromA. hydrophilaobtained from a tilapia (Oreochromis niloticus) farm in Thailand. pR148 is a 165,906-bp circular plasmid containing 147 coding regions showing highest similarity to pNDM-1_Dok1, an MDR plasmid isolated from a human pathogen. pR148 was also very similar to other IncA/C plasmids isolated from humans, animals, food, and fish. pR148 contains a mercuric resistance operon and encodes the complete set of genes for the type 4 secretion system. pR148 encodes a Tn<I>21</I>type transposon. This transposon contains the drug resistance genes<I>qacH</I>,<I>bla</I>OXA-10,<I>aadA1</I>, and<I>sul1</I>in a class 1 integron;<I>tetA</I>and<I>tetR</I>in transposon Tn<I>1721</I>; and<I>catA2</I>and a duplicate<I>sul1</I>in a locus showing 100% similarity to IncU plasmids isolated from fish. The<I>bla</I>OXA-10and<I>aadA1</I>genes showed 100% similarity to those from theAcinetobacter baumanniiAYE genome. The similarity of pR148 to a human pathogen-derived plasmid indicates that the plasmids were either transferred between different genera or that they are derived from a common origin. Previous studies have shown that IncA/C plasmids retain a conserved backbone, while the accessory region points to lateral gene transfer. These observations point out the dangers of indiscriminate use of antibiotics in humans and in animals and the necessity of understanding how drug resistance determinants are disseminated and transferred.</P>
Kim, Young Kyu,Lee, Jung Seok,Jung, Jae Wook,Hikima, Jun-ichi,Ohtani, Maki,Jang, Ho Bin,Nho, Seong Won,Cha, In Seok,Park, Seong Bin,Lee, Jeong Ho,Aoki, Takashi,Jung, Tae Sung Elsevier 2017 FISH AND SHELLFISH IMMUNOLOGY Vol.60 No.-
<P><B>Abstract</B></P> <P>Immunoglobulins (Ig) are heterodimeric proteins that play critical roles in the adaptive immune system of vertebrates. Because of their plasticity, teleostean Igs are more diverse, and thus do not conform to mammalian classifications. Because of this, mammalian-based Ig cell markers cannot be used successfully to study immune responses in fish. There is therefore a need to produce Ig-specific cell markers for fish. Here, we attempted to identify the specific isotype detected by an Ig light chain-specific monoclonal antibody (anti-olive flounder IgL-mAb: M7C3-4) that we had previously produced [11]. Three newly identified sequences of the Ig light chain from olive flounder were classified according to their isotypes. Subsequent analyses revealed that M7C3-4 was able to specifically detect lymphocytes expressing one of the κ chains (Igκ-a) in olive flounder. Interestingly, Igκ-a<SUP>+</SUP> B cells were more abundant in spleen and trunk-kidney than in peripheral blood, indicating a distribution different from that of IgM<SUP>+</SUP> B cells. Our work reveals interesting aspects of B cell distribution and differentiation, and may aid in the production of suitable and effective cell markers for olive flounder.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Olive flounder has three isotypes of IgL, two of κ, and one of σ. </LI> <LI> M7C3-4 produced against serum immunoglobulins of olive flounder specifically recognizes Igκ-a isotype. </LI> <LI> Igκ-a+ B cells are more abundant in organs than in peripheral blood. </LI> </UL> </P>