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Young-Jun Park,Tomotaro Nishikawa,Kazuhiro Nemoto,Kenichi Matsushima,Mineo Minami,Makoto Kawase 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
We investigated the expression patterns of a granule-bound starch synthase I (GBSSI = Waxy) gene at different developmental stages of storage and non-storage organs in Amaranthus cruentus. GBSSI transcripts were strongly expressed in the middle and mid-late stages of seed development and thereafter expression decreased. In addition, this gene was expressed in all non-storage organs tested (the leaf, stem, petiole and root) and showed a tendency to increase during plant development. Therefore, our results indicate that the amaranth GBSSI gene exhibits late expression in the perisperm, and that it is expressed in both storage and non-storage tissues. We also investigated the genetic diversity of GBSSI among 37 strains of amaranth grains originating from New World. A comparison of the GBSSI coding sequence revealed an extremely high level of sequence conservation, and a single nucleotide polymorphism between the sequences of non-waxy (Type I) and waxy (Type II) phenotypes was detected. This indicates that a G–T polymorphism in exon 10 (a nonsense mutation) was a unique event in the evolution of the GBSSI gene in amaranth grains.
Atsushi Goto,Takeshi Okamoto,Ryo Ogawa,Kouichi Hamabe,Shinichi Hashimoto,Jun Nishikawa,Taro Takami 대한소화기내시경학회 2022 Clinical Endoscopy Vol.55 No.4
Background/Aims: Intralesional steroid injections have been administered as prophylaxis for stenosis after esophageal endoscopicsubmucosal dissection. However, this method carries a risk of potential complications such as perforation because a fine needle is usedto directly puncture the postoperative ulcer. We devised a new method of steroid intralesional infusion using a spray tube and evaluatedits efficacy and safety. Methods: Intralesional steroid infusion using a spray tube was performed on 27 patients who underwent endoscopic submucosal dissectionfor superficial esophageal cancer with three-quarters or more of the lumen circumference resected. The presence or absence ofstenosis, complications, and the number of endoscopic balloon dilations (EBDs) performed were evaluated after treatment. Results: Although stenosis was not observed in 22 of the 27 patients, five patients had stenosis and dysphagia requiring EBD. The stenosisin these five patients was relieved after four EBDs. No complications related to intralesional steroid infusion using the spray tubewere observed. Conclusions: Intralesional steroid infusion using a spray tube is a simple and safe technique that is adequately effective in preventingstenosis (clinical trial number, UMIN000037567).