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Genome-wide identification of pepper NB-LRR gene family and their evolutionary history in Solanaceae
Eunyoung Seo,Seon-In Yeom,Seungill Kim,Joohyun Lee,Saet-Byul Kim,Eunbi Choi,Eun Hye Choi,Doil Choi 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
Plants have evolved elaborate innate immune systems against invading pathogens, such as bacteria, fungi, oomycetes, viruses and insects. Among them, intracellular immune receptors known as nucleotide-binding site and leucine-rich repeat (NB-LRR) play critical roles in effector-triggered immunity (ETI) regarding to plant defense. Here, we identified potential NB-LRR coding sequences from pepper genome using bioinformatics analysis and performed comparative analysis with Solanaceae plants. As a result, we identified 267, 443, and 755 NBS-encoding genes in the genome of tomato, potato, and pepper, respectively. These may indicate that the Solanaceae NB-LRRs were evolved through species-specific unequal-duplication event. Further phylogenetic and clustering analyses revealed that Solanaceae NB-LRRs were classified into the 14 subgroups with 1 TNL and 13 CNL types. We found that the genes in CNL-G1 and CNL-G2 subgroup were highly expanded compared to other subgroup showing a large portion of NB-LRR in pepper genome. Among 755 NB-LRRs in pepper genome, 623 were physically mapped on all 12 pepper chromosome pseudomolecules. Furthermore, a number of NB-LRRs in the same group were physically clustered by tandem array in the specific chromosome. Genome-wide identification of pepper NB-LRR family and their evolutionary analysis could provide an important resource for identification and characterization of genes for breeding of disease resistance crops.
장주현(Joohyun Jang),손혁호(Hyukho Son),서동수(Dongsu Seo),서현준(Hyunjun Seo) 한국자동차공학회 2019 한국자동차공학회 학술대회 및 전시회 Vol.2019 No.11
In this study, we want to deal with the chassis fastening part where loosening problem occurred due to improper fastening setting. The cause of problem was dissatisfaction due to poor inner dimensions of the brackets and excessive deviations. Through analysis, the amount of clamping force loss in the fastened object was derived. And through the bolt fastening test, fastening information such as torque, clamping force, angle was obtained. These data were analyzed to establish the angle tightening specification, and the actual product tightening test was used to confirm the effect of reducing the clamping force distribution.
Kang, Joohyun,Lee, Yewon,Choi, Yukyung,Kim, Sejeong,Ha, Jimyeong,Oh, Hyemin,Kim, Yujin,Seo, Yeongeun,Park, Eunyoung,Rhee, Min Suk,Lee, Heeyoung,Yoon, Yohan The Korean Society of Fisheries and Aquatic Scienc 2021 Fisheries and Aquatic Sciences Vol.24 No.2
The annual consumption of fishery products, particularly sea squirt (Halocynthia roretzi), per person has steadily increased in South Korea. However, the quantitative risk of Vibrio parahaemolyticus following intake of sea squirt has not been analyzed. This study focuses on quantitative predictions of the probability of consuming sea squirt and getting of V. parahaemolyticus foodborne illness. The prevalence of V. parahaemolyticus in sea squirt was evaluated, and the time spent by sea squirt in transportation vehicles, market displays, and home refrigerators, in addition to the temperature of each of these, were recorded. The data were fitted to the @RISK program to obtain a probability distribution. Predictive models were developed to determine the fate of V. parahaemolyticus under distribution conditions. A simulation model was prepared based on experimental data, and a dose-response model for V. parahaemolyticus was prepared using data from literature to estimate infection risk. V. parahaemolyticus contamination was detected in 6 of 35 (17.1%) sea squirt samples. The daily consumption quantity of sea squirt was 62.14 g per person, and the consumption frequency was 0.28%. The average probability of V. parahaemolyticus foodborne illness following sea squirt consumption per person per day was 4.03 × 10<sup>-9</sup>. The objective of this study was to evaluate the risk of foodborne illness caused by Vibrio parahaemolyticus following sea squirt consumption in South Korea.
Finger Clamping Unit: A Clamping Device with a Large Clamping Range
Youngjae Jeon,Joohyun Oh,Jongwon Kim,Tae Won Seo 한국정밀공학회 2021 International Journal of Precision Engineering and Vol.22 No.2
Finger Clamping Unit (FCU) is a small-sized single-point clamp developed to clamp thin object of various thicknesses. It has a six-bar linkage with one compliant link, uses an electric motor as an actuator and induces toggle-lock motion by singular configuration. The main purpose of the FCU is to clamp car bodies in car assembly lines, while adapting to various thicknesses of car body. Unlike other clamping devices in the industry, it has a large clamping range (defined as the thickness range that the FCU can clamp). In this paper, the overall process of FCU development is presented. The six-bar linkage was chosen in type synthesis, and compliance was added for toggle-lock motion. Static analysis of the compliant FCU was completed, and results were plotted in a three-dimensional (3D) plot which expresses the behavior of the FCU. The lengths of each link were optimized in dimension synthesis. An FCU was constructed to reinforce the simulated results. The unique design of both the six-bar linkage FCU and the compliant link were presented. Simulated and experimental 3D plots were compared and analyzed. An appropriate friction model that best fits the experiment data was found. The advantage of the FCU—its clamping range—was proved by experiment.