Assessment of the Cytotoxic and Apoptotic Effects of Chaetominine in a Human Leukemia Cell Line

Jing-Yun Yao,Ruihua Jiao,Changqing Liu,Yupeng Zhang,Wan-Guo Yu,Yan-Hua Lu,Renxiang Tan 한국응용약물학회 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.2

Chaetominine is a quinazoline alkaloid originating from the endophytic fungus Aspergillus fumigatus CY018. In this study, we showed evidence that chaetominine has cytotoxic and apoptotic effects on human leukemia K562 cells and investigated the pathway involved in chaetominine-induced apoptosis in detail. Chaetominine inhibited K562 cell growth, with an IC50 value of 35 nM, but showed little inhibitory effect on the growth of human peripheral blood mononuclear cells. The high apoptosis rates, morphological apoptotic features, and DNA fragmentation caused by chaetominine indicated that the cytotoxicity was partially caused by its pro-apoptotic effect. Under chaetominine treatment, the Bax/Bcl-2 ratio was upregulated (from 0.3 to 8), which was followed by a decrease in mitochondrial membrane potential, release of cytochrome c from mitochondria into the cytosol, and stimulation of Apaf-1. Furthermore, activation of caspase-9 and caspase-3, which are the main executers of the apoptotic process, was observed. These results demonstrated that chaetominine induced cell apoptosis via the mitochondrial pathway. Chaetominine inhibited K562 cell growth and induced apoptotic cell death through the intrinsic pathway, which suggests that chaetominine might be a promising therapeutic for leukemia.

Assessment of the Cytotoxic and Apoptotic Effects of Chaetominine in a Human Leukemia Cell Line

( Jing Yun Yao ),( Rui Hua Jiao ),( Chang Qing Liu ),( Yu Peng Zhang ),( Wan Guo Yu ),( Yan Hua Lu ),( Ren Xiang Tan ) 한국응용약물학회 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.2

Chaetominine is a quinazoline alkaloid originating from the endophytic fungus Aspergillus fumigatus CY018. In this study, we showed evidence that chaetominine has cytotoxic and apoptotic effects on human leukemia K562 cells and investigated the pathway involved in chaetominine-induced apoptosis in detail. Chaetominine inhibited K562 cell growth, with an IC50 value of 35 nM, but showed little inhibitory effect on the growth of human peripheral blood mononuclear cells. The high apoptosis rates, morphological apoptotic features, and DNA fragmentation caused by chaetominine indicated that the cytotoxicity was partially caused by its pro-apoptotic effect. Under chaetominine treatment, the Bax/Bcl-2 ratio was upregulated (from 0.3 to 8), which was followed by a decrease in mitochondrial membrane potential, release of cytochrome c from mitochondria into the cytosol, and stimulation of Apaf-1. Furthermore, activation of caspase-9 and caspase-3, which are the main executers of the apoptotic process, was observed. These results demonstrated that chaetominine induced cell apoptosis via the mitochondrial pathway. Chaetominine inhibited K562 cell growth and induced apoptotic cell death through the intrinsic pathway, which suggests that chaetominine might be a promising therapeutic for leukemia.

A "Dynamic Form-Finding" Approach to Environmental-Performance Building Design

Yao, Jia-Wei,Lin, Yu-Qiong,Zheng, Jing-Yun,Yuan, Philip F. Council on Tall Building and Urban Habitat Korea 2018 International journal of high-rise buildings Vol.7 No.2

Newly-designed high-rise buildings, both in China and abroad, have demonstrated new innovations from the creative concept to the creative method. from the creative concept to the creative method. At the same time, digital technology has enabled more design freedom in the vertical dimension. "Twisting" has gradually become the morphological choice of many city landmark buildings in recent years. The form seems more likely to be driven by the interaction of aesthetics and structural engineering. Environmental performance is often a secondary consideration; it is typically not simulated until the evaluation phase. Based on the research results of "DigitalFUTURE Shanghai 2017 Workshop - Wind Tunnel Visualization", an approach that can be employed by architects to design environmental-performance buildings during the early stages has been explored. The integration of a dynamic form-finding approach (DFFA) and programming transforms the complex relationship between architecture and environment into a dialogue of computer language and dynamic models. It allows the design to focus on the relationship between morphology and the surrounding environment, and is not limited to the envelope form itself. This new concept of DFFA in this research consists of three elements: 1) architectural form; 2) integration of wind tunnel and dynamic models; and 3) environmental response. The concept of wind tunnel testing integrated with a dynamic model fundamentally abandons the functional definition of the traditional static environment simulation analysis. Instead it is driven by integral environmental performance as the basic starting point of morphological generation.

Solute Distribution Ahead of Interface during Heteroepitaxial Growth

Yao, Wen Jing,Jin, Zeng Jia,Wang, Nan,Lee, Jeh Yun Trans Tech Publications 2013 Advanced materials research Vol.690 No.-

<P>By establishing a diffusion model, the solute distribution is investigated during the growth of eutectic phase from the binary melt on the primary phase. Based on the eutectic reaction conditions, reasonable assumptions are carried out to build the partial differential equation. After the determination of the initial and boundary conditions, the analytical solutions have been derived from the method of variables separation. The solute distribution ahead of interface and the influence of this distribution on the growth rate of heteroepitaxial phase are discussed.</P>

Characteristics of wind loading on internal surface and its effect on wind-induced responses of a super-large natural-draught cooling tower

Yun-feng Zou,Zheng-yi Fu,Xu-hui He,Hai-quan Jing,Ling-yao Li,Hua-wei Niu,Zheng-qing Chen 한국풍공학회 2019 Wind and Structures, An International Journal (WAS Vol.29 No.4

Wind loading is one of important loadings that should be considered in the design of large hyperbolic natural-draught cooling towers. Both external and internal surfaces of cooling tower are under the action of wind loading for cooling circulating water. In the previous studies, the wind loads on the external surface attracted concernedly attention, while the study on the internal surface was relatively ware. In the present study, the wind pressure on the internal surface of a 220 m high cooling tower is measured through wind tunnel testing, and the effect of ventilation rate of the packing layer on internal pressure is a major concern. The characteristics of internal wind pressure distribution and its effect on wind-induced responses calculated by finite element method are investigated. The results indicate that the wind loading on internal surface of the cooling tower behaves remarkable three-dimensional effect, and the pressure coefficient varies along both of height and circumferential directions. The non-uniformity is particularly strong during the construction stage. Analysis results of the effect of internal pressure on wind-induced responses show that the size and distribution characteristics of internal pressure will have some influence on wind-induced response, however, the outer pressure plays a dominant role in the wind-induced response of cooling tower, and the contribution of internal pressure to the response is small.

Influence of Rear Earth Yb on the Specific Heat of Liquid Fe-Co-Yb Alloy

Yao, Wen Jing,Wang, Nan,Lee, Jeh Yun Trans Tech Publications 2013 Advanced materials research Vol.690 No.-

<P>By Monte Carlo method with EAM potentials, the specific heat of liquid Co50Fe50 and Co48Fe48Th4 alloys at different temperatures are obtained. The effect of Th on the thermophysical parameters is examined. Over the temperature range from 1400 to 2000 K, the specific heat decreases from to Jmol<SUP>-1</SUP>K<SUP>-1</SUP> after Th was added. It is the addition of Th element which has bigger atomic radius, larger atomic mass, and complex arrangement of extranuclear electron, leads to the reduced specific heat.</P>

Separation of Nattokinase from Bacillus subtilis Fermentation Broth by Expanded Bed Adsorption with Mixed-mode Adsorbent

Shan-Jing Yao,Miao-Hua Lu,Dong-Qiang Lin,Yuan-Chun Wu,Jun-Xian Yun,Le-He Mei 한국생물공학회 2005 Biotechnology and Bioprocess Engineering Vol.10 No.2

Mixed-mode hydrophobic/ionic matrices exhibit a salt-tolerant property for adsorbing target protein from high-ionic strength feedstock, which allows the application of undiluted feedstock via an expanded bed process. In the present work, a new type of mixed-mode adsorbent designed for expanded bed adsorption, Fastline PRO, was challenged for the capture of nattokinase from the high ionic fermentation broth of Bacillus subtilis. Two important factors, pH and ion concentration, were investigated with regard to the performance of nattokinase adsorption. Under initial fermentation broth conditions (pH 6.6 and conductivity of 10 mS/cm) the adsorption capacity of nattokinase with Fastline PRO was high, with a maximum capacity of 5,350 U/mL adsorbent. The elution behaviors were investigated using packed bed adsorption experiments, which demonstrated that the effective desorption of nattokinase could be achieved by effecting a pH of 9.5. The biomass pulse response experiments were carried out in order to evaluate the biomass/adsorbent interactions between Bacillus subtilis cells and Fastline PRO, and to demonstrate a stable expanded bed in the feedstock containing Bacillus subtilis cells. Finally, an EBA process, utilizing mixed-mode Fastline PRO adsorbent, was optimized to capture nattokinase directly from the fermentation broth. The purification factor reached 12.3, thereby demonstrating the advantages of the mixed-mode EBA in enzyme separation.

Iron-Nitrogen Embedded into Porous N-Doped Carbon Catalysts for Catalytic Oxidation Processes

Mao Jing YU,Feng Yu WEI,Yun Jin YAO 대한환경공학회 2019 대한환경공학회 학술발표논문집 Vol.2019 No.-

Nitrogen-doped Carbon Encapsulating Metal Nanostructures Loaded with Membrane for Cr<SUP>VI </SUP>Reduction

Mao Jing YU,Ze Heng Yang,Yun Jin YAO 대한환경공학회 2019 대한환경공학회 학술발표논문집 Vol.2019 No.-

Landscape structures of the Central Yangtze region, China

Tang, Zhi Yao,Fang, Jing Yun,Zhao, Shu Qing,Rao, Sheng,Lei, Guang Chun,Cui, Hai Ting 대한원격탐사학회 2000 International Symposium on Remote Sensing Vol.16 No.1

As habitats for many migratory birds, the plain regions of Central Yangtze plays an important role in biodiversity conservation, especially in conservation for birds and fishes. In this study, the landscape structures of the plain regions of the Central Yangtze were analyzed by using remote sensing image data (Landsat TM) and technique of geographical information system (GIS). The landscape component was divided into eight types, broad-leaved forest, shrub, meadow, water body, cropland, urban and bared land. The main results are summarized as follow: (1) Broad-leaved forest is almost made of small patches, while water body and cropland mainly made of large-area patches, suggesting that water body and cropland are the dominant components for landscape structures. (2) Shrub and meadow are highly fragmented, compared to cropland and water body. Water body, meadow and shrub are randomly distributed, whilst bared land and urban occur aggregately. (3) Places with high fragmentation index show a low dominance index value and unapparent dominant components, while the areas with low fragmentation indices, such as Dongting Lake, Poyang Lake and Jianghan Plain, indicate a high dominance index value in which the dominant components were distinct.