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백롱민,박재현,김진환,백세민,오갑성,정연철 大韓成形外科學會 1993 Archives of Plastic Surgery Vol.20 No.5
The shape and contour of the upper third of the face is provided basically by the frontal bone. A relatively thin soft tissue coverage, which consists of the skin and frontalis muscle, readily shows subtle changes on the underlying frontal bone. The forehead represents the fortune of one's youth from the viewpoint of physignomy, and a broad forehead with minimal supraorbital bossing and round curvature is preferrd in the Orient. The forehead with a deficient, flat contour or asymmetry could be indicated for augmentation. The exact site and amount of augmentation are dicided preoperatively using clinical assessment, medical photographs, and cephalometric analysis. Hydroxyapatite(Pyrost), because of its nonresorbability, seems to have advantages over other alloplastic materials for forehead augmentation. From March 1989 to February 1992 forehead augmentation has been performed in 60 patients. Through a coronal incision, the dissection was carried over the periosteum to the supraorbital ridge and the lateral orbital rim. The subperiosteal pocket was made through a small incision, and was packed with Pyrost. When indicated, facial rhytidectomy, or reduction malar plasty was performed simultaneously. The result had been satisfactory and long lasting. There had been no implant shifting, infection, or bony erosion when examed from 1 year to 4 years after surgery.
Proteomics Analysis of Porcine Oocyte Proteins
Rong Xun Han,Hong Rye Kim,Yun Fei Diao,Jin Yu Zhang,Min Gu Lee,Chang Sik Park,Dong Il Jin 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1
An understanding of oocyte gene expression is a necessary for the study of biological development. Recently, Oocyte has been used in many techniques such as somatic cell nuclear transfer (SCNT), intracytoplasmic sperm injection (ICSI) and embryonic stem cell derivation. However, the molecular mechanism underlying porcine oocyte is still unclear. In this study, we present the description of the porcine oocyte proteome. Proteins within the isoelectric point ranges of 3.0 to 10.0 were analyzed separately using 2‐dimensional electrophoresis (2‐DE). About 450 spots were detected in 2‐ D gel of oocytes, stained with Coomassie blue. Subsequent excision of 227 spots from gels and MALDI‐TOF MS analysis allowed the identification of 85 proteins. Our results indicated the composite profiles of proteins in the porcine oocyte. Tubulin beta chain and meiosis‐specific nuclear structural protein 1 antibody was used to confirm those antibody expression levels in immature, mature and parthenogenetic embryo. Western blot analysis showed that expressions of those proteins increased during mature and parthenogenetic embryo. These protein profiles will make available important guides for the study of oocyte function and assist in functional analysis of the proteins.
A Composition Method of Logical Function Block Chain
JIN Rong,HE Xiong-xiong,Zhu Guang-xin 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.3
ForCES is a new open programmable architecture, it separates control and forwarding, it also abstracts resources of Forwarding Elements (FE) into some Logical Function Blocks (LFB), Control Element (CE) can reconfigure the forwarding function of FEs by recomposing their LFB chains. Firstly, A SDN architecture based on ForCES is proposed. Then, based on the formalization of the concept of LFB and the traditional I/O matching algorithm, an LFB chain composition method is proposed. This method can combine a series of LFBs to an LFB chain according to special application request. Based on the LFB chain composition algorithm, an improved algorithm is proposed, which can improve the composition efficiency. At last, an example is provided to illustrate how this method works, and a simulation is given to compare the efficiency of the base algorithm and the improved algorithm.
Jin, Hai-Rong,Kim, Woo Jean,Song, Jae Sook,Piao, Shuguang,Choi, Min Ji,Tumurbaatar, Munkhbayar,Shin, Sun Hwa,Yin, Guo Nan,Koh, Gou Young,Ryu, Ji-Kan,Suh, Jun-Kyu American Diabetes Association 2011 Diabetes Vol.60 No.3
<P><B>OBJECTIVE</B></P><P>Patients with diabetic erectile dysfunction often have severe endothelial dysfunction and respond poorly to oral phosphodiesterase-5 inhibitors. We examined the effectiveness of the potent angiopoietin-1 (Ang1) variant, cartilage oligomeric matrix protein (COMP)-Ang1, in promoting cavernous endothelial regeneration and restoring erectile function in diabetic animals.</P><P><B>RESEARCH DESIGN AND METHODS</B></P><P>Four groups of mice were used: controls; streptozotocin (STZ)-induced diabetic mice; STZ-induced diabetic mice treated with repeated intracavernous injections of PBS; and STZ-induced diabetic mice treated with COMP-Ang1 protein (days −3 and 0). Two and 4 weeks after treatment, we measured erectile function by electrical stimulation of the cavernous nerve. The penis was harvested for histologic examinations, Western blot analysis, and cGMP quantification. We also performed a vascular permeability test.</P><P><B>RESULTS</B></P><P>Local delivery of the COMP-Ang1 protein significantly increased cavernous endothelial proliferation, endothelial nitric oxide (NO) synthase (NOS) phosphorylation, and cGMP expression compared with that in the untreated or PBS-treated STZ-induced diabetic group. The changes in the group that received COMP-Ang1 restored erectile function up to 4 weeks after treatment. Endothelial protective effects, such as marked decreases in the expression of p47<SUP>phox</SUP> and inducible NOS, in the generation of superoxide anion and nitrotyrosine, and in the number of apoptotic cells in the corpus cavernosum tissue, were noted in COMP-Ang1–treated STZ-induced diabetic mice. An intracavernous injection of COMP-Ang1 completely restored endothelial cell-cell junction proteins and decreased cavernous endothelial permeability. COMP-Ang1–induced promotion of cavernous angiogenesis and erectile function was abolished by the NOS inhibitor, <I>N</I>-nitro-L-arginine methyl ester, but not by the NADPH oxidase inhibitor, apocynin.</P><P><B>CONCLUSIONS</B></P><P>These findings support the concept of cavernous endothelial regeneration by use of the recombinant Ang1 protein as a curative therapy for diabetic erectile dysfunction.</P>
Overexpression of IbMPK3 increases low-temperature tolerance in transgenic sweetpotato
Jin Rong,Kim Ho Soo,Yu Tao,Liu Ming,Yu Wenhui,Zhao Peng,Zhang Aijun,Zhang Qiangqiang,Liu Yaju,Cao Qinghe,Kwak Sang-Soo,Tang Zhonghou 한국식물생명공학회 2022 Plant biotechnology reports Vol.16 No.1
Sweetpotato is an important crop that is very sensitive to low temperatures. Mitogen-activated protein kinase (MAPK) is involved in plant growth and development and is responsive to many environmental stresses. IbMPK3 is strongly regulated by low-temperature stress. We studied the function of IbMPK3 and how it may enhance the adaptability of sweetpotato plants to low-temperature stress. Transgenic sweetpotato plants overexpressing IbMPK3 were generated, and three trans- genic lines with the highest expression level of IbMPK3 were used for low-temperature tests. Transgenic plants exposed to low-temperature stress had less damage associated with higher photosynthesis efficiency and less cell membrane damage. IbMPK3-overexpressing transgenic plants could modulate reactive oxygen species (ROS) metabolism with lower levels of O2− and H2O2 accumulation and higher enzymatic activities than WT plants. Transcript expression levels of some ROS- related genes (IbCAT, IbCu-ZnSOD, and IbCAT) and stress-responsive genes (IbP3B and IbCOR27) were significantly upregulated in transgenic plants compared to WT. These results indicate that IbMPK3 has an important role in sweetpotato response to low temperatures.