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- 저자 : Jiarui Du (검색결과 3 건)
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Genetic transformation of wheat: current status and future prospects
Jiarui Li,Xingguo Ye,Baoyan An,Lipu Du,Huijun Xu 한국식물생명공학회 2012 Plant biotechnology reports Vol.6 No.3
Genetic transformation is a reverse genetics tool for validation of target genes and crop improvement. However, due to its low efficiency and genotype dependency,wheat is considered a recalcitrant plant for genetic transformation. During the last 20 years, various in vitro and in planta transformation methods have been reported in wheat. Until now, biolistic particle and Agrobacteriummediated wheat transformation methods using immature embryos as explants have been the two major transformation approaches. In addition to immature embryos, other explant types, such as mature embryos, anther-derived calli, inflorescences, apical meristems, and other floral organs, have been employed; however, they need further optimization. In addition to the common marker genes,such as bar, hpt and gus, other effective markers, ALS,AtMYB12 and pmi, have been successfully used for selection of positive transgenic plants. Numerous agronomic trait genes such as biotic stress resistance or tolerance genes have been transferred into wheat plants. Future prospects, such as recipient wheat cultivars and explants,marker free issues, and transgene silencing, are discussed. The objective of this review is to summarize current successful techniques for wheat transformation and stimulate further research into long-term wheat improvement by genetic engineering approaches.
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Xiaokang Yu,Jinsheng Liang,Jiarui Xu,Xingsong Li,Shan Xing,Huilan Liu,Wan-Li Liu,Dongdong Liu,Jianhua Xu,Lizhen Huang,Hongli Du 한국유방암학회 2018 Journal of breast cancer Vol.21 No.4
Purpose: Breast cancer is the most commonly occurring cancer among women worldwide, and therefore, improved approaches for its early detection are urgently needed. As microRNAs (miRNAs) are increasingly recognized as critical regulators in tumorigenesis and possess excellent stability in plasma, this study focused on using miRNAs to develop a method for identifying noninvasive biomarkers. Methods: To discover critical candidates, differential expression analysis was performed on tissue-originated miRNA profiles of 409 early breast cancer patients and 87 healthy controls from The Cancer Genome Atlas database. We selected candidates from the differentially expressed miRNAs and then evaluated every possible molecular signature formed by the candidates. The best signature was validated in independent serum samples from 113 early breast cancer patients and 47 healthy controls using reverse transcription quantitative real-time polymerase chain reaction. Results: The miRNA candidates in our method were revealed to be associated with breast cancer according to previous studies and showed potential as useful biomarkers. When validated in independent serum samples, the area under curve of the final miRNA signature (miR-21-3p, miR-21-5p, and miR-99a-5p) was 0.895. Diagnostic sensitivity and specificity were 97.9% and 73.5%, respectively. Conclusion: The present study established a novel and effective method to identify biomarkers for early breast cancer. And the method, is also suitable for other cancer types. Furthermore, a combination of three miRNAs was identified as a prospective biomarker for breast cancer early detection.
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Experimental Study on Lime-Treated Waste Soil Based on Water Transfer Mechanism
Fanlu Min,Jiawei Ma,Nan Zhang,Hangbiao Song,Jiarui Du,Dengfeng Wang 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.5
A large amount of waste soil (mud) is being produced from the construction of civil and infrastructures. As a result, resource utilization of the waste soil has become even more important in geotechnical engineering community. This study quantitatively investigates the underlying modification mechanism of lime-treated waste soil based on water transfer mechanism. Content of different types of water was first measured, and content of each hydration product and the Atterberg limits of soils before and after the lime treatment were then analyzed. It is found the mechanism of lime treatment is to transfer free water with low potential energy to bound water and hydration water with high potential energy. In addition, the formation of hydrates gel mainly improves soil plasticity by affecting the limit of soil plasticity. The hydration water can be used to evaluate the effectiveness of treatment and the growth potential.
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