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        Axial compressive behavior of concrete-filled steel tube columns with stiffeners

        Wei Liang,Jiangfeng Dong,Qingyuan Wang 국제구조공학회 2018 Steel and Composite Structures, An International J Vol.29 No.2

        In order to reduce the deformation and delay the local buckling of concrete filled steel tube (CFST) columns, strengthening the structures with stiffeners is an effective method. In this paper, a new stiffening method with inclined stiffeners was used to investigate the behaviors of short CFST columns under axial compression. Besides, a three-dimensional nonlinear finite element (FE) model was applied to simulate the mechanical performances, including the total deformation, local buckling, and stress-strain relationship. Revised constitutive models of stiffened steel tube and confined concrete are proposed. A good agreement was achieved between the test and FE results. Furthermore, the calculated results of load capacity by using a simplified method also show a good correlation with experimental data.

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        Recovery of mortar-aggregate interface of fire-damaged concrete after post-fire curing

        Lang Li,Hong Zhang,Jiangfeng Dong,Hongen Zhang,Pu Jia,Qingyuan Wang,Yongjie Liu 사단법인 한국계산역학회 2019 Computers and Concrete, An International Journal Vol.24 No.3

        In order to investigate the strength recovery of fire-damaged concrete after post-fire curing, concrete specimens were heating at 2°C/min or 5°C/min to 400, 600 and 800°C, and these exposed specimens were soaked in the water for 24 hours and following by 29-day post-fire curing. The compressive strength and split tensile strength of the high-temperature-exposed specimens before and after post-fire curing were tested. The proportion of split aggregate in the split surfaces was analyzed to evaluate the mortar-aggregate interfacial strength. After the post-fire curing process, the split tensile strength of specimens exposed to all temperatures was recovered significantly, while the recovery of compressive strength was only obvious within the specimens exposed to 600°C. The tensile strength is more sensitive to the mortar-aggregate interfacial cracks, which caused that the split tensile strength decreased more after high-temperature exposure and recovery more after post-fire curing than the compressive strength. The mortar-aggregate interfacial strength also showed remarkable recovery after post-fire curing, and it contributed to the recovery of split tensile strength.

      • KCI등재

        Simulation study on CFRP strengthened reinforced concrete beam under four-point bending

        Dongliang Zhang,Qingyuan Wang,Jiangfeng Dong 사단법인 한국계산역학회 2016 Computers and Concrete, An International Journal Vol.17 No.3

        This paper presents numerical modeling of the structural behavior of CFRP (carbon fiber reinforced polymer) strengthened RC (reinforced concrete) beams under four-point bending. Simulation of debonding at the CFRP-concrete interface was focused, as it is the main failure mode of CFRP strengthened RC beams. Here, cohesive layer was employed to model the onset of debonding, which further helps to describe the post debonding behavior of the CFRP strengthened RC beam. In addition, the XFEM approach was applied to investigate the effects of crack localization on strain field on CFRP sheet and rebar. The strains obtained from the XFEM correlate better to the test results than that from CDP (concrete damaged plasticity) model. However, there is a large discrepancy between the experimental and simulated loaddisplacement relationships, which is due to the simplification of concrete constitutive law.

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        Performance and Mechanism Analysis of Succinate Production under Different Transporters in Escherichia coli

        Xiaozhan Li,Wenming Zhang,Mingke Wu,Fengxue Xin,Weiliang Dong,Hao Wu,Min Zhang,Jiangfeng Ma,Min Jiang 한국생물공학회 2017 Biotechnology and Bioprocess Engineering Vol.22 No.5

        Succinic acid is a platform chemical with potential for bio-based synthesis. However, the production of bio-based succinate is limited because of insufficient succinate efflux capacity in the late stage of fermentation. In the present study, three different transporters, which have been reported to be responsible for C4-dicarboxylates transport, were employed for investigation of the transport capacity of succinate in Escherichia coli. After engineered strains were constructed, the fermentative production of succinic acid was studied in serum bottles and 3 L of fermentor. The results demonstrated that engineered strain showed better efflux capacity than control strain under high concentration of succinate. The highest production of succinate was 68.66 g/L, while the NCgl2130 transporter may be the best candidate for succinate export in E. coli. Further research showed that the expression levels and relative enzyme activities involved in the metabolic pathway all increased markedly, and the maximum activities of PPC, PCK, PYK, and MDH increased by 1.50, 1.38, 1.28, and 1.27-fold in recombinant E. coli AFP111/pTrc99a- NCgl2130, respectively. Moreover, the maximum level of intracellular ATP increased by 23.79% in E. coli AFP111/ pTrc99a-NCgl2130. Taken together, these findings indicated that engineered transporters can improve succinate production by increasing key enzyme activities and intracellular ATP levels. To the best of thew authors’ knowledge, this is the first report on a mechanism to improve succinate production by engineered transporters. This strategy set up a foundation for improving the biosynthesis of other C4-dicarboxylates, such as fumaric acid and malic acid.

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