Effects of crude extracted proteins from supernatant of BCF-P and EGF on acid secretion

Yuqing Zhao,Tao Guo,Jiaming Qian 대한독성 유전단백체 학회 2015 Molecular & cellular toxicology Vol.11 No.3

I n order to compare the in vitro effects of crude extracts of broth culture filtrate protein (BCF-P) from the supernatant of an H. pylori liquid culture and epidermal growth factor (EGF) on acid secretion by isolated rabbit gastric parietal cells. Isolated rabbit gastric parietal cells were incubated with BCF-P or EGF for 1 and 12 h. Morphological changes were assessed by electron microscopy, and 14C-aminopyrine (14C-AP) uptake and H+-K+ATP mRNA expression were measured. At 50 μg/mL, BCF-P stimulated acid secretion by parietal cells (P<0.05) at 1 h but not at 12 h. At 100 μg/mL, BCF-P significantly inhibited histamine-stimulated acid secretion (P<0.05) and decreased H+- K+ATP mRNA expression in parietal cells, but it was reversed after EGF exposure. Crude extracted proteins from BCF-P damaged the inner cell membrane structure of parietal cells, reduced H+-K+ATP gene expression, and inhibited acid secretion, activities that differed markedly from those of EGF.

PREPARATION OF FOLATE-CONJUGATED BOVINE SERUM ALBUMIN NANOPARTICLES ADSORBING EPIRUBICIN HYDROCHLORIDE

XIUHUA ZHAO,JIAMING TANG,YUANGANG ZU,WEIGUO WANG,NA LI,WENJIA XU 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2013 NANO Vol.8 No.6

This work investigated the preparation process of folate-conjugated bovine serum albumin nanoparticles (FA–BSANPs) adsorbing epirubicin hydrochloride (EPI) nanoparticles (FA–EPI–BSANPs), a specific-targeting drug delivery system in cancer chemotherapy. The BSANPs were prepared by desolvation as a drug carrier system and conjugated with folate to produce FA–EPI–BSANPs that specifically target tumors by cross-linking. EPI, an anticancer drug, was adsorbed by this drug carrier system. The influences of six experimental parameters, namely, the adsorption time, FA–BSANPs solution-adsorbed EPI concentration, stirring speed, FA–BSANPs solution pH, the ratio of glutaraldehyde and BSA, and mass ratio of FA–BSANPs to EPI, on the drug loading efficiency (DLR) and drug entrapment efficiency (DER) of FA–EPI–BSANPs were investigated via the single factor method. The results indicated that the optimum operation conditions were obtained with 145.4 nm±0.5 nm MPS, 23.41% DLR and 98.93% DER. The N-hydroxysuccinimide-folate content associated with BSANPs was up to 0.9757% (wt). The DLR and DER of EPI increased with increasing adsorption time, FA–BSANPs solution concentration, and pH value, peaking at 1750 rpm with increasing stirring speed, but decreasing thereafter. The FA–EPI–BSANPs obtained were characterized by laser light scattering, scanning electron microscopy, Fourier transform infrared spectroscopy, differential scanning calorimetry, X-ray diffraction and thermogravimetric analysis. Drug release in vitro was investigated, as well. The characterization results showed that EPI in FA–EPI–BSANPs existed in an amorphous, instead of crystalline state. Most of the EPI was enclosed by FA–BSANPs, and a small amount was adsorbed onto the surface of the FA–BSANPs. The FA–EPI–BSANPs particles are nearly ellipsoidal and significantly affect sustained release. The inhibitory rate of FA–EPI–BSANP was mensurated by MTT method. The inhibitory rate of FA–EPI–BSANPs for SMMC 7721 cell developed with raise of concentration and was higher than other samples. The IC50 values of FA–EPI–BSANPs and EPI were 11.5 μg/mL and 18.8 μg/mL, respectively. The target ability of FA–EPI–BSANP for SMMC 7721 cell was mensurated by fluorescence (FITC) modified albumin techniques. The uptake rate of FA–EPI–BSANPs was higher than samples without folate conjugated, and increased with increased concentration.

Efficacy evaluation of novel organic iron complexes in laying hens: effects on laying performance, egg quality, egg iron content, and blood biochemical parameters

Jiuai Cao,Jiaming Zhu,Qin Zhou,Luyuan Zhao,Chenhao Zou,Yanshan Guo,Brian Curtin,Fei Ji,Bing Liu,Dongyou Yu Asian Australasian Association of Animal Productio 2023 Animal Bioscience Vol.36 No.3

Objective: This study was conducted to determine the optimal dose of novel iron amino acid complexes (Fe-Lys-Glu) by measuring laying performance, egg quality, egg iron (Fe) concentrations, and blood biochemical parameters in laying hens. Methods: A total of 1,260 18-week-old healthy Beijing White laying hens were randomly divided into 7 groups with 12 replicates of 15 birds each. After a 2-wk acclimation to the basal diet, hens were fed diets supplemented with 0 (negative control, the analyzed innate iron content was 75.06 mg/kg), 15, 30, 45, 60, and 75 mg Fe/kg as Fe-Lys-Glu or 45 mg Fe/kg from FeSO₄ (positive control) for 24 wk. Results: Results showed that compared with the negative and positive control groups, dietary supplementation with 30 to 75 mg Fe/kg from Fe-Lys-Glu significantly (linear and quadratic, p<0.05) increased the laying rate (LR) and average daily egg weight (ADEW); hens administered 45 to 75 mg Fe/kg as Fe-Lys-Glu showed a remarkable (linear, p<0.05) decrease in feed conversion ratio. There were no significant differences among all groups in egg quality. The iron concentrations in egg yolk and serum were elevated by increasing Fe-Lys-Glu levels, and the highest iron content was found in 75 mg Fe/kg group. In addition, hens fed 45 mg Fe/kg from Fe-Lys-Glu had (linear and quadratic, p<0.05) higher yolk Fe contents than that with the same dosage of FeSO₄ supplementation. The red blood cell (RBC) count and hemoglobin content (linear and quadratic, p<0.05) increased obviously in the groups fed with 30 to 75 mg Fe/kg as Fe-Lys-Glu in comparison with the control group. Fe-Lys-Glu supplementation also (linear and quadratic, p<0.05) enhanced the activity of copper/zinc-superoxide dismutase (Cu/Zn-SOD) in serum, as a result, the serum malonaldehyde content (linear and quadratic, p<0.05) decreased in hens received 60 to 75 mg Fe/kg as Fe-Lys-Glu. Conclusion: Supplementation Fe-Lys-Glu in laying hens could substitute for FeSO₄ and the optimal additive levels of Fe-Lys-Glu are 45 mg Fe/kg in layers diets based on the quadratic regression analysis of LR, ADEW, RBC, and Cu/Zn-SOD.

Preparation and Characterization of PLA Film/3D Printing Composite Scaffold for Tissue Engineering Application

Chenjie Meng,Jiaming Zhao,Yuxiang Yin,Jun Luo,Lianying Zhao,Wenbin Jiang,Jianyong Feng 한국섬유공학회 2020 Fibers and polymers Vol.21 No.4

Three-dimensional (3D) printing technology provides an effective alternative for tissue engineering scaffold. Inthis paper, a composite scaffold composed of polylactic acid (PLA) film and 3D printing scaffold was fabricated which couldprovide structural and mechanical support for tissue application. The effects of PLA film/3D printing composite scaffold onscaffold morphology, average diameter, pore diameter, mechanical properties, themal conductivity, electrical conductivity,and surface hydrophilicity were characterized. PLA film/3D printing composite scaffold had larger thickness, bettermechanical and thermal conductivity properties in comparison with pure PLA film and 3D printed scaffold. These resultssuggested that composite scaffold with relatively good overall performance could be a potential candidate for tissueengineering application.

Multi-scale Nano/Micro Fiber Scaffolds with Different Topological Morphologies

Jianyong Feng,Ruixiang Xu,Jiaming Zhao,Lingxiao Zhang 한국섬유공학회 2022 Fibers and polymers Vol.23 No.4

The multi-scale fiber and topological morphologies had been shown to influence cell mitogenesis and chemotaxis,direct cell differentiation, and induce constructive host tissue remodeling responses. To simulate multi-layer meshworkarchitecture of extracellular matrix and regulate cell behavior, we prepared different topological scaffolds, such as 3Dprinting fiber scaffolds, poly(lactic acid) (PLA) electrospun fiber scaffolds, 3D printing/knitted fabric composite scaffolds,and PLA electrospinning/knitted fabric composite scaffolds. The surface morphology, pore diameter, thickness, stress-straincurve, electrical current, and other properties were studied. The aim is to prepare multi-scale nano/micro fiber scaffolds withdifferent topological morphologies and properties that can provide structural and mechanical support for potential tissueengineering applications.

T-spline surface smoothing based on 1-ring neighborhood space angle

Wang Aizeng,Li Ling,Chang Heng,Zhao Gang,Wang Wei,Yang Jiaming 한국CDE학회 2022 Journal of computational design and engineering Vol.9 No.4

The prominent properties owned by T-spline, such as flexibility, continuity, local refinement, water tightness, make it extensively applied in Computer Aided Design (CAD)- and Computer Aided Engineering (CAE)-integrating scenarios. But the local fairness may dissatisfy in damaged areas or even on the entire surface of industry applications. Under these circumstances, local protrusion and sharp features appeared seriously affect the fairness of T-spline surfaces. Derived from the geometric properties of T-spline control points, we propose a smoothing algorithm based on the 1-ring neighborhood space angle to deal with local abruptions of T-spline surfaces. We also demonstrate the availability of the proposed algorithm through several experiments. Results show that this method is suitable for removing sharp features and smoothing unstructured T-spline surfaces.

Therapeutic responses to chemotherapy or immunotherapy by molecular subtype in bladder cancer patients: A meta-analysis and systematic review

Shunde Wang,Xiaoyu Yuan,Zhongjie Shen,Jiaming Zhao,Baishu Zheng,Junyong Zhang,Chengguo Ge 대한비뇨의학회 2023 Investigative and Clinical Urology Vol.64 No.3

To systematically evaluate the differences in therapeutic response to chemotherapy or immunotherapy between different molecular subtypes of bladder cancer (BC). A comprehensive literature search was performed up to December 2021. Consensus clusters 1 (CC1), CC2 and CC3 molecular subtypes were used to perform meta-analysis. Pooled odds ratios (ORs) with 95% confidence intervals (CIs) were used to assess the therapeutic response by fix-effect modeling. Eight studies involving 1,463 patients were included. For immunotherapy, CC3 showed the highest response rate (CC1 vs. CC3: OR=0.52, 95% CI=0.34–0.78, p=0.002; CC2 vs. CC3: OR=0.42, 95% CI=0.28-0.62, p<0.001), which was mainly reflected in the highest response rate to atezolizumab (CC1 vs. CC3: OR=0.47, 95% CI=0.29–0.75, p=0.002; CC2 vs. CC3: OR=0.38, 95% CI=0.24–0.59, p<0.001). For chemotherapy, CC3 had the lowest response rate to the overall chemotherapy (CC1 vs. CC3: OR=2.05, 95% CI=1.23–3.41, p=0.006; CC2 vs. CC3: OR=2.48, 95% CI=1.50–4.10, p<0.001). Compared with CC2, CC3 responded poorly to both neo-adjuvant chemotherapy (NAC) (OR=1.93, 95% CI=1.09–3.41, p=0.020) and chemoradiation therapy (CRT) (OR=6.07, 95% CI=1.87–19.71, p<0.001). Compared with CC1, CC3 only showed a poorer response to CRT (OR=4.53, 95% CI=1.26–16.27, p=0.020), and no difference in NAC. Our study suggested that molecular classifications are important predictors of cancer treatment outcomes of BC patients and could identify subgroup patients who are most likely to benefit from specific cancer treatments.

Comparison of accuracy between digital and conventional implant impressions: two and three dimensional evaluations

Chuang Bi,Xingyu Wang,Fangfang Tian,Zhe Qu,Jiaming Zhao 대한치과보철학회 2022 The Journal of Advanced Prosthodontics Vol.14 No.4

PURPOSE. The present study compared the accuracy between digital and conventional implant impressions. MATERIALS AND METHODS. The experimental models were divided into six groups depending on the implant location and the scanning span. Digital impressions were captured using the intraoral optical scanner TRIOS (3Shape, Copenhagen, Denmark). Conventional impressions were taken with the monophase impression material based on addition-cured silicones, Honigum-Mono (DMG, Hamburg, Germany). A highprecision laboratory scanner D900 (3Shape, Copenhagen, Denmark) was used to obtain digital data of resin models and stone casts. Surface tessellation language (STL) datasets from scanner were imported into the analysis software Geomagic Qualify 14 (3D Systems, Rock Hill, SC, USA), and scan body deviations were determined through two-dimensional and three-dimensional analyses. Each scan body was measured five times. The Sidak t test was used to analyze the experimental data. RESULTS. Implant position and scanning distance affected the impression accuracy. For a unilateral arch implant and the mandible models with two implants, no significant difference was observed in the accuracy between the digital and conventional implant impressions on scan bodies; however, the corresponding differences for trans-arch implants and mandible with six implants were extremely significant (P <.001). CONCLUSION. For short-span scanning, the accuracy of digital and conventional implant impressions did not differ significantly. For long-span scanning, the precision of digital impressions was significantly inferior to that of the traditional impressions.

Hepatotoxic mechanism of diclofenac sodium on broiler chicken revealed by iTRAQ-based proteomics analysis

Sun, Chuanxi,Zhu, Tianyi,Zhu, Yuwei,Li, Bing,Zhang, Jiaming,Liu, Yixin,Juan, Changning,Yang, Shifa,Zhao, Zengcheng,Wan, Renzhong,Lin, Shuqian,Yin, Bin 대한수의학회 2022 Journal of Veterinary Science Vol.23 No.3

Background: At the therapeutic doses, diclofenac sodium (DFS) has few toxic side effects on mammals. On the other hand, DFS exhibits potent toxicity against birds and the mechanisms remain ambiguous. Objectives: This paper was designed to probe the toxicity of DFS exposure on the hepatic proteome of broiler chickens. Methods: Twenty 30-day-old broiler chickens were randomized evenly into two groups (n = 10). DFS was administered orally at 10mg/kg body weight in group A, while the chickens in group B were perfused with saline as a control. Histopathological observations, serum biochemical examinations, and quantitative real-time polymerase chain reaction were performed to assess the liver injury induced by DFS. Proteomics analysis of the liver samples was conducted using isobaric tags for relative and absolute quantification (iTRAQ) technology. Results: Ultimately, 201 differentially expressed proteins (DEPs) were obtained, of which 47 were up regulated, and 154 were down regulated. The Gene Ontology classification and Kyoto Encyclopedia of Genes and Genomes pathway analysis were conducted to screen target DEPs associated with DFS hepatotoxicity. The regulatory relationships between DEPs and signaling pathways were embodied via a protein-protein interaction network. The results showed that the DEPs enriched in multiple pathways, which might be related to the hepatotoxicity of DFS, were "protein processing in endoplasmic reticulum," "retinol metabolism," and "glycine, serine, and threonine metabolism." Conclusions: The hepatotoxicity of DFS on broiler chickens might be achieved by inducing the apoptosis of hepatocytes and affecting the metabolism of retinol and purine. The present study could provide molecular insights into the hepatotoxicity of DFS on broiler chickens.

Hepatotoxic mechanism of diclofenac sodium on broiler chicken revealed by iTRAQ-based proteomics analysis

Chuanxi Sun,Tianyi Zhu,Yuwei Zhu,Bing Li,Jiaming Zhang,Yixin Liu,Changning Juan,Shifa Yang,Zengcheng Zhao,Renzhong Wan,Shuqian Lin,Bin Yin 대한수의학회 2022 Journal of Veterinary Science Vol.23 No.4

Background: At the therapeutic doses, diclofenac sodium (DFS) has few toxic side effects on mammals. On the other hand, DFS exhibits potent toxicity against birds and the mechanisms remain ambiguous. Objectives: This paper was designed to probe the toxicity of DFS exposure on the hepatic proteome of broiler chickens. Methods: Twenty 30-day-old broiler chickens were randomized evenly into two groups (n = 10). DFS was administered orally at 10 mg/kg body weight in group A, while the chickens in group B were perfused with saline as a control. Histopathological observations, serum biochemical examinations, and quantitative real-time polymerase chain reaction were performed to assess the liver injury induced by DFS. Proteomics analysis of the liver samples was conducted using isobaric tags for relative and absolute quantification (iTRAQ) technology. Results: Ultimately, 201 differentially expressed proteins (DEPs) were obtained, of which 47 were up regulated, and 154 were down regulated. The Gene Ontology classification and Kyoto Encyclopedia of Genes and Genomes pathway analysis were conducted to screen target DEPs associated with DFS hepatotoxicity. The regulatory relationships between DEPs and signaling pathways were embodied via a protein-protein interaction network. The results showed that the DEPs enriched in multiple pathways, which might be related to the hepatotoxicity of DFS, were “protein processing in endoplasmic reticulum,” “retinol metabolism,” and “glycine, serine, and threonine metabolism.” Conclusions: The hepatotoxicity of DFS on broiler chickens might be achieved by inducing the apoptosis of hepatocytes and affecting the metabolism of retinol and purine. The present study could provide molecular insights into the hepatotoxicity of DFS on broiler chickens.