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Eye and Skin Irritation Tests for Cell Wall Glucan and Mannoproteins, from Saccharomyces cerevisiae
Chang Hoon Ha,Hye Young Kim,Cheol Won Youn,Hyun Dong Paik,Seung Wook Kim,Chang Won Kang,Hyo-Ihl Chang 한국실험동물학회 2005 Laboratory Animal Research Vol.21 No.3
Yeast cell wall matrix particles are entirely composed of mannoprotein and β-glucan. Yeast cell wall β-glucans and mannoproteins were isolated and purified from cell wall mutants in the previous study (Ha et al., 2002). The mannoproteins and the alkali-soluble glucan of yeast cell wall have been shown to systemically enhance immune system function. These nonspecific immunostimulators was evaluated for primary skin and eye irritation in male New Zealand White rabbits. In skin irritation test of β-glucans and mannoproteins, body weights were not significantly changed and there were no responses after treatment for 24 hours and the primary irritation index (P.I.I.) was ‘0’. And, in the eye irritation test, there were no chemosis in any rabbits in washing group and non-washing group. Taken together, these results indicate that yeast cell wall β-glucans and mannoproteins may be non-irritant materials.
Immune-Enhancing Alkali-Soluble Glucans Produced by Wild-Type and Mutant Saccharomyces cerevisiae
( Chang Hoon Ha ),( Ki Hong Lim ),( Se Hwan Jang ),( Cheol Won Yun ),( Hyun Dong Paik ),( Seung Wook Kim ),( Chang Won Kang ),( Hyo Ihl Chang ) 한국미생물생명공학회 2006 Journal of microbiology and biotechnology Vol.16 No.4
Streptococcus salivarius subsp. thermophilus가 생산하는 Bacteriocin의 특성
이장혁,장효일 한국산업미생물학회 1994 한국미생물·생명공학회지 Vol.22 No.1
Antagonism assay를 하여 원유에서 억제환의 크기가 가장 크며 단백질 가수분해 효소에 의해 항균 능력이 상실되는 균을 분리하여 Streptococcus salivarius subsp.thermophilus로 동정하였다. 이 균주는 45℃에서 specific growth rate가 가장 높은 반면 bacteriocin을 생산하지 않았다. 이 bacteriocin은 고온에서도 상당한 열안정성을 보였으며 단백질 가수분해 효소에 의해 활성이 상실되었으나 α-amylase, DNaseI, RNase는 bacteriocin활성에 영향을 주지 못하였다. One bacterial strain, that had made the largest inhibition zone at the antagonism assay and also that lost the inhibition activity by the protease treatment, was isolated from raw milk. That strain was identified as Streptococcus salivarius subsp. thermophilus. The specific growth rate of this strain was maximum at 45℃. However, at this temperature the strain produced no bacteriocin. The bacteriocin activity was quite stable even at high temperature. Moreover, the activity of the bacteriocin was sensitive to proteases, but not to α-amylase, DNase I, or RNase.
Identification of Isoleucine - Accepting tRNA in Maize Mitochondria
Park, Young In,Chang, Hyo Ihl,Lee, Byung Chul,Moon, A Ree 생화학분자생물학회 1979 BMB Reports Vol.28 No.6
Maize mitochondrial tRNAs for isoleucine have been isolated using a putative tRNA^(Ile) gene probe which has been previously isolated and characterized. It contains the 5'-CAT anticodon which would normally recognize the AUG methionine codon. The nucleotide sequence of one of these tRNAs has been partially determined, and contains a modified nucleotide at the first position of the anticodon. This type of posmanscriptional modification event could change the specificity of amino acid acceptance of a tRNA, unlike that deduced from the corresponding gene. An aminoacylation experiment also demonstrated that these purified tRNAs have isoleucine acceptance activity but no methionine-accepting activity.
Sung, Ha Chin,Chang, Hyo Ihl,Kim, Min Jung,Kim, Young Woo,Lee, Jin Young 생화학분자생물학회 1981 BMB Reports Vol.29 No.5
Bacteriophage ΦFCl is a temperate phage which was identified as a prophage in the Enterococcus faecalis KBL703 chromosome. Phage ΦFCl integrates into the host chromosome by site-specific recombination. The phage attachment site P (attP) was localized within the 0.65-kb XhoI-HindIII fragment and the nucleotide sequence of the region was determined. An open reading frame (mj1) which adjoined the phage attachment site encoded a deduced protein related to the site-specific recombinase family. The organization of this region was comparable to other site-specific recombination systems. The molecular weight of the expressed MJ1 in E. coli was in good agreement with the predicted 53,537 Da of the mj1 gene product. Elucidation of the phage-specific integration process in this study would provide useful genetic tools such as a chromosomal integration system.
Byong Chul Lee,Hyo Ihl Chang,Young In Park 생화학분자생물학회 1994 BMB Reports Vol.27 No.3
A genomic library was constructed by cloning large fragments of maize mtDNA into pBR322. The fragments were produced by partial digestion with HindIII. Clone H606/01 contained a 20 kb mtDNA insert consisting of two HindIII fragments of 9 kb and 11 kb. The clone was also found to harbor an elongator methionine tRNA^(Met) gene. This elongator tRNA^(Met) gene is exactly identical to its chloroplast counterparts both in rice and maize, with some variation in the flanking regions. The restriction map for the flanking regions of the gene was compared with that of chloroplasts, and the entire 9 kb HindIII fragment insert was identical to that of cpDNA. This suggests that it is another example of a cpDNA insert present in mtDNA.