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Hyeong Gon Yu,Hum Chung,Young Suk Yu,Jong Mo Seo,Jang Won Heo 대한안과학회 2003 Korean Journal of Ophthalmology Vol.17 No.1
AlamarBlue is used to induce color and fluorescence in the microenvironment of activated cells. The alamarBlue assay was studied to determine if it could be used as a method of evaluating the number of retinal pigment epithelial (RPE) cells. A series of two-fold dilutions of RPE cells were placed into 96-well culture plates. The alamarBlue was added to the culture media after attaching the cells. The absorbance and fluorescence were measured consecutively at various intervals over a period of 24 hr. Cell viability were evaluated by means of the trypan blue exclusion method and flow cytometry using a combination of propidium iodide and annexin V was done to prove the safety of alamarBlue assay to the cells. Both the absorbance and the fluorescence had a linear relationship with the number of RPE cells. Exposing the RPE cells to alamarBlue was not detrimental to the cells. In conclusion, the alamarBlue assay constitutes a one-step, extremely simple, reproducible, economical and non-toxic procedure for evaluating the number of viable RPE cells.
유형곤(Hyeong Gon Yu),윤창기(Chang Ki Yoon) 대한검안학회 2021 Annals of optometry and contact lens Vol.20 No.3
망막색소변성 환자는 시야결손, 색각이상, 대비감도 감소, 읽기와 운전장애 등 다양한 시각 이상과 기능적 시각 이상을 나타낸다. 망막색소변성 환자에 대한 시기능 이해는 치료 효과를 판정하는 임상시험에서 필수적으로 고려되어야 한다. 또한 시각장애 판정 등에서 망막변성 환자가 불이익을 당하지 않도록 도와줄 수 있을 것이다. Patients with retinitis pigmentosa have defects in visual function, including the visual field, color vision, contrast sensitivity, and impaired functional vision, such as reading and driving. An assessment of visual function is required when interpreting the outcome of clinical trials of retinitis pigmentosa. Various defects of functional vision should be considered when assessing visual impairment for patients with retinitis pigmentosa.