Combined Genotype Analyses of Precursor miRNA-196a2 and -499a Variants with Hepatic and Renal Cancer Susceptibility- a Preliminary Study

Toraih, Eman A,Fawzy, Manal S,Elgazzaz, Mona G,Hussein, Mohammad H,Shehata, Rasha H,Daoud, Hisham G Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.7

MicroRNAs, a novel class of small non-coding RNAs, are key players in many cellular processes, including cell proliferation, differentiation, invasion and regeneration. Tissue and circulatory microRNAs could serve as useful clinical biomarkers and deregulated expression levels have been observed in various cancers. Gene variants may alter microRNA processing and maturation. Thus, we aimed to investigate the association of MIR-196a2 rs11614913 (C/T), MIR-499a rs3746444 (A/G) polymorphisms and their combination with cancer susceptibility in an Egyptian population. Sixty five renal cell carcinoma (RCC) and 60 hepatocellular carcinoma (HCC) patients and 150 controls were enrolled in the study. They were genotyped using real-time polymerase chain reaction technology. Both $miR-196a2^*T$ and $miR-499a*G$ were associated with RCC risk, but only $miR-196a^*T$ was associated with HCC development. Carriage of the homozygote combinations ($MIR196a2^*TT+MIR499a^*AA$) and ($MIR196a2^*CC+MIR499a^*GG$) was associated with 25 and 48 fold elevation of likelhood to develop RCC, respectively. The miR-196a2 SNP was also linked with larger tumor size in RCC and advanced tumor stage in HCC. miR-196a2 and miR-499a combined genotypes were associated with RCC and HCC. Further functional analysis of SNPs is required to confirm relationships between genotypes and phenotypes.

A five-variable refined plate theory for thermal buckling analysis of composite plates

Hashim, Hussein A.,Sadiq, Ibtehal Abbas Techno-Press 2021 Composite materials and engineering Vol.3 No.2

This research is devoted to investigate the thermal buckling analysis behaviour of laminated composite plates, by applying an analytical model based on a refined plate theory (RPT) with five independent unknown variables. The theory accounts for parabolic distribution of the transvers shear strains through the plate thickness, and satisfied the zero traction boundary condition on the surface without using shear correction factors, hence a shear correction factor is not required. The governing differential equations and associated boundary conditions are derived by employing the principle of virtual work and solved via Navier-type analytical procedure to obtain critical buckling temperature for simply supported boundary condition of symmetric and antisymmetric cross-ply and angle-ply laminated plates. MATLAB 2018 program is used to investigate the effect of thickness ratio (a/h), aspect ratio (a/b), orthogonality ratio (E₁/E₂), coefficient of thermal expansion ratio (α₂/α₁) and numbers of layers on thermal buckling of laminated plate. It can be concluded that this theory gives good results when compared with other theory.

Effects of Several Effective Microorganisms (EM) on the Growth of Chinese cabbage (Brassica rapa)

Hussein, Khalid A.,Joo, Jin-Ho Korean Society of Soil Science and Fertilizer 2011 한국토양비료학회지 Vol.44 No.4

The development of satisfactory alternatives for supplying the nutrients needed by crops could decrease the problems associated with conventional NPK chemical fertilizers. In this study, the effects of bacterial and fungal effective microorganisms (EM) on the growth of Chinese cabbage (Brassica rapa) were evaluated. This investigation was carried out parrallel with conventional NPK chemical fertilizer and a commercial sold microbial fertilizer to compare between each of their effect. Sterile water and molasses were served as controls. Azotobacter chroococcum effect also was studied either alone or in combination with the effective microorganisms on the growth parameters. In contrast to the bacterial EM, the fungal EM alone without A. chroococcum had a more stimulating effect than fungal EM combined with A. chroococcum. Results showed that seedling inoculation significantly enhanced B. rapa growth. Shoot dry and fresh weight, and leaf length and width significantly were increased by both bacterial and fungal inoculation. The results indicated that the NPK chemical fertilizer deteriorates the microflora inhabiting the soil, while the effective microorganisms either fungal or bacterial ones increased the microbial density significantly. This study implies that both of fungal and bacterial EM are effective for the improvement of the Chinese cabbage growth and enhance the microorganisms in soil. The results showed antagonism occurred between A. chroococcum and each of Penicillium sp and Trichoderma sp in both agar and plant assays. The data were statistically analyzed by ANOVA and Dunnett test.

Effects of Several Effective Microorganisms (EM) on the Growth of Chinese cabbage (Brassica rapa)

Khalid A. Hussein,주진호 한국토양비료학회 2011 한국토양비료학회지 Vol.44 No.4

The development of satisfactory alternatives for supplying the nutrients needed by crops could decrease the problems associated with conventional NPK chemical fertilizers. In this study, the effects of bacterial and fungal effective microorganisms (EM) on the growth of Chinese cabbage (Brassica rapa) were evaluated. This investigation was carried out parrallel with conventional NPK chemical fertilizer and a commercial sold microbial fertilizer to compare between each of their effect. Sterile water and molasses were served as controls. Azotobacter chroococcum effect also was studied either alone or in combination with the effective microorganisms on the growth parameters. In contrast to the bacterial EM, the fungal EM alone without A. chroococcum had a more stimulating effect than fungal EM combined with A. chroococcum. Results showed that seedling inoculation significantly enhanced B. rapa growth. Shoot dry and fresh weight, and leaf length and width significantly were increased by both bacterial and fungal inoculation. The results indicated that the NPK chemical fertilizer deteriorates the microflora inhabiting the soil, while the effective microorganisms either fungal or bacterial ones increased the microbial density significantly. This study implies that both of fungal and bacterial EM are effective for the improvement of the Chinese cabbage growth and enhance the microorganisms in soil. The results showed antagonism occurred between A. chroococcum and each of Penicillium sp and Trichoderma sp in both agar and plant assays. The data were statistically analyzed by ANOVA and Dunnett test.

Effect of Chromium Chloride on the Mechanical and Dielectric Properties of EPDM Rubber

M.A.Salem,M.A.Khaled,A.M.Hussein,E.Elway 한국고분자학회 2003 Macromolecular Research Vol.11 No.5

Measurements of Young’s modulus, dielectric loss and a.c. conductivity have been carried out on EPDM rubber samples loaded with different concentrations of CrCl3 (0,2,4 and 6 phr). The values of Young’s modulus was found to be linearly dependent on the CrCl3 content. Variation of the dielectric loss with temperature showed that CrCl3 may act as plasticizer. However, at higher frequencies the dielectric loss was found to be independent of frequency and the rubber samples may behave as non-polar dielectric. Investigations of the a.c conductivity suggested that the conduction in these rubber samples can be described by small polaron tunneling. In addition, conductivity was found to increase with CrCl3 content.

Effect of Chromium Chloride on the Mechanical and Dielectric Properties of EPDM Rubber

Salem, M.A.,Khaled, M.A.,Hussein, A.M.,Elway, E. The Polymer Society of Korea 2003 Macromolecular Research Vol.11 No.4

Measurements of Young's modulus, dielectric loss and a.c. conductivity have been carried out on EPDM rubber samples loaded with different concentrations of $CrCl_3$ (0,2,4 and 6 phr). The values of Young's modulus was found to be linearly dependent on the $CrCl_3$ content. Variation of the dielectric loss with temperature showed that $CrCl_3$ may act as plasticizer. However, at higher frequencies the dielectric loss was found to be independent of frequency and the rubber samples may behave as non-polar dielectric. Investigations of the a.c conductivity suggested that the conduction in these rubber samples can be described by small polaron tunneling. In addition, conductivity was found to increase with $CrCl_3$ content.

Antimicrobial Efficacy of Allium cepa and Zingiber officinale Against the Milk-Borne Pathogen Listeria monocytogenes

Arasu Abirami,Prabha Nagaram,Devi Durga,Issac Praveen Kumar,Alarjani Khaloud Mohammed,Al Farraj Dunia A.,Aljeidi Reem A.,Tayyeb Jehad Zuhair,Guru Ajay,Arockiaraj Jesu,Mohan Magesh,Hussein Dina S. 한국미생물학회 2023 The journal of microbiology Vol.61 No.11

Listeria monocytogenes is an important food-borne pathogen that causes listeriosis and has a high case fatality rate despite its low incidence. Medicinal plants and their secondary metabolites have been identified as potential antibacterial substances, serving as replacements for synthetic chemical compounds. The present studies emphasize two significant medicinal plants, Allium cepa and Zingiber officinale, and their efficacy against L. monocytogenes. Firstly, a bacterial isolate was obtained from milk and identified through morphology and biochemical reactions. The species of the isolate were further confirmed through 16S rRNA analysis. Furthermore, polar solvents such as methanol and ethanol were used for the extraction of secondary metabolites from A. cepa and Z. officinale. Crude phytochemical components were identified using phytochemical tests, FTIR, and GC–MS. Moreover, the antibacterial activity of the crude extract and its various concentrations were tested against L. monocytogenes. Among all, A. cepa in methanolic extracts showed significant inhibitory activity. Since, the A. cepa for methanolic crude extract was used to perform autography to assess its bactericidal activity. Subsequently, molecular docking was performed to determine the specific compound inhibition. The docking results revealed that four compounds displayed strong binding affinity with the virulence factor Listeriolysin-O of L. monocytogenes. Based on the above results, it can be concluded that the medicinal plant A. cepa has potential antibacterial effects against L. monocytogenes, particularly targeting its virulence.

Tolerance to Salt Stress by Plant Growth-Promoting Rhizobacteria on Brassica rapa var. glabra

Hussein, Khalid A.,Yoo, Jaehong,Joo, Jin Ho Korean Society of Soil Science and Fertilizer 2016 한국토양비료학회지 Vol.49 No.6

Salinity has been a threat to agriculture in some parts of the world; and recently, the threat has grown. Plant growth-promoting rhizobacteria (PGPR) may benefit plant growth, either by improving plant nutrition or producing plant growth hormones. The effects of rhizobacterial strains to attenuate the salinity stress on the germination of Chinese cabbage seeds were tested using four different concentrations of NaCl (50, 100, 150, and 200 mM). Also, PGPR strains were tested to enhance the early germination of Chinese cabbage seeds under normal conditions. Azotobacter chroococcum performed best with enhancing the radicle length of 4.0, 1.2, and 1.0 times at treatments of 50, 100, and 150 mM of NaCl, respectively. Additionally, significant differences were found in plumule length, A. chroococcum and Lactobacillus sp. showed remarkable activation either in normal or under stress conditions. Co-inoculation by three rhizobacterial strains (LAPmix) indicated synergistic effect to enhance the early germination of the seeds. The results of this study are promising for application of rhizobacterial strains that possess plant growth promoting traits to enhance the plant tolerance against salinity.

Chemical composition, aphicidal and antiacetylcholinesterase activities of essential oils against Aphis nerii Boyer de Fonscolombe (Hemiptera: Aphididae)

Hussein Hanaa S.,Tawfeek Mohamed E.,Abdelgaleil Samir A.M. 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.2

Aphids are important sucking insects that attack many crops and cause huge economic loss. Essential oils have been proposed to be appropriate alternative to synthetic insecticides due to their low impact on environment and human health. In this work, the chemical compositions of eight essential oils extracted from plants growing in Egypt were determined by gas chromatography/mass spectrometry (GC–MS). The aphicidal activities of the isolated oils were examined against the adults of oleander aphid, Aphis nerii by a leaf-dipping assay. The inhibitory effect of essential oils on acetylcholinesterase (AChE) activity was also tested in vitro. Chemical an alyses showed the essential oils consisted mainly of monoterpene hydrocarbons, oxygenated monoterpenes, sesquiterpene hydrocarbons and oxygenated sesquiterpenes except the oil of Artemisia monosperma which con tained high content of benzenes. The results of toxicity assay showed that the oils of A. monosperma and Citrus sinensis were the most potent toxicants displaying LC 50 value of 0.06 mg/L. The oils of Callistemon viminals, Schinus terebinthifolius and Schinus molle were also highly effective against aphid as their LC 50 values were lower than 1 mg/L. In addition, the highest inhibitory effect on AChE was recorded by 0.5 mg/L C. sinensis with in hibition of 49.33%, while 0.1 mg/L Citrus lemon oil recorded the lowest inhibitory effect with inhibition of 0.4%. In general, six of tested essential oils showed high toxicity against A. nerii and they might be applied in the IPM programs for this insect.

Tolerance to Salt Stress by Plant Growth-Promoting Rhizobacteria on Brassica rapa var. glabra

Khalid A. Hussein,Jaehong Yoo,Jin Ho Joo 한국토양비료학회 2016 한국토양비료학회지 Vol.49 No.6

Salinity has been a threat to agriculture in some parts of the world; and recently, the threat has grown. Plant growth-promoting rhizobacteria (PGPR) may benefit plant growth, either by improving plant nutrition or producing plant growth hormones. The effects of rhizobacterial strains to attenuate the salinity stress on the germination of Chinese cabbage seeds were tested using four different concentrations of NaCl (50, 100, 150, and 200 mM). Also, PGPR strains were tested to enhance the early germination of Chinese cabbage seeds under normal conditions. Azotobacter chroococcum performed best with enhancing the radicle length of 4.0, 1.2, and 1.0 times at treatments of 50, 100, and 150 mM of NaCl, respectively. Additionally, significant differences were found in plumule length, A. chroococcum and Lactobacillus sp. showed remarkable activation either in normal or under stress conditions. Co-inoculation by three rhizobacterial strains (LAPmix) indicated synergistic effect to enhance the early germination of the seeds. The results of this study are promising for application of rhizobacterial strains that possess plant growth promoting traits to enhance the plant tolerance against salinity.