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Qun Huang,Shu-gang Li,Hui Teng,Yong-guo Jin,Mei-hu Ma,Hong-bo Song 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.6
Angiotensin-I-converting enzyme (ACE) inhibitory peptides were prepared from ovalbumin using enzyme hydrolysis with pepsin as an enzyme source. Effects of pH, enzyme dosage, substrate concentration, hydrolysis temperature, and time on the degree of hydrolysis and the ACE inhibition rate were investigated using single factor experiments. Preparation conditions for ACE inhibitory peptides were optimized using a response surface design on the base of single factor experiments. Optimum preparation conditions were a substrate concentration of 5.2 g/100 mL of D.W with a pH value of 2.5, an enzyme dosage of 14,000 U/g, and a hydrolysis time of 250 min at 30℃. The ACE inhibition rate was up to 70.55±1.13% under these conditions.
Performance and applications of flow-guided sieve trays for distillation of highly viscous mixtures
Hui Liu,Qun Shen Li,Chun Ying Song,Hai Long Wu,Yi Qiu Qian 한국화학공학회 2008 Korean Journal of Chemical Engineering Vol.25 No.6
With a particular focus on the distillation of highly viscous or self-polymerized mixtures, this study reports the hydrodynamic and mass transfer performance of two flow-guided sieve trays, including their pressure drop, entrainment, weeping and tray efficiency, obtained experimentally with an air-water/oxygen system in a Φ600 mm plexiglass column. The results show that the 8 mm hole flow-guided sieve tray tested shows better characteristics than the 7 mm flow-guided hole tray in terms of pressure drop and mass transfer. Then we present practical industrial examples of applications of the flow guided trays for distillation of viscous mixtures, i.e., the separation of vinyl acetate (VAC) from a polyvinyl acetate (PVAC) polymer solution with dynamic viscosity μ=50,000 mPas, the separation of highly unsaturated C5 mixtures by extractive distillation, and the distillation of thick, condensed and highly viscous fermentation mixtures made from fermented mash. It is demonstrated that flow-guided sieve trays with relatively large holes are an excellent candidate for distillation of mixtures with suspended solids, or concentrated/self-polymerized polymer solutions.
Dong-Hui Hao,Xin Song,Jian-Qiang Lin,Yu-Jie Su,Yin-Bo Qu,Jian-Qun Lin 한국생물공학회 2008 Biotechnology and Bioprocess Engineering Vol.13 No.1
In this study, we describe the isolation and identification of a novel long-chain n-alkane degrading strain, Gordonia amicalis LH3. Under aerobic conditions, it utilized approximately 18.0% of paraffin (2% w/v) after 10 day of incubation, and the paraffin compositions of C18C24 alkalines were utilized preferentially. Under anaerobic conditions, paraffin utilization was approximately 1/8 that seen under aerobic conditions, and the compositions of C34 and C36 alkalines were utilized preferentially. The effects of salinity, temperature, and biosurfactants on paraffin degradation were also evaluated. The strain was also demonstrated to grow on oil, and decreased oil viscosity by 44.7% and degraded oil by 10.4% under aerobic conditions. Our results indicated that G. amicalis LH3 has potential applications in paraffin control, microbial enhanced oil recovery (MEOR), and the bioremediation of hydrocarbon-polluted environments.
Xu, Hui-Min,Zhu, Jian-Guo,Gu, Lian,Hu, Song-Qun,Wu, Hao Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.4
Vascular endothelial growth factor 2 (VEGFR2) was initially identified as a receptor of VEGF on endothelial cells with a role in regulating angiogenesis during organism development and tumorigenesis. Previously, in cancer tissue, VEGFR2 has been reported to be expressed in endothelial cells. In our research, we found that VEGFR2 was expressed not only in endothelial cells but also cancer cells in head and neck squamous cell carcinomas (HNSCCs). Knockdown of VEGFR2 in Hep2 cells could arrest the cell cycle in G0/G1, leading to a decrease in proliferation. We also present evidence that MAPK/ERK signal pathways and expression of CDK1 downstream of VEGFR2 might regulate proliferation and cell cycle arrest. Furthermore, we discovered that down-regulate VEGRF2 in Hep2 cells could significantly affect the invasion ability. Taken together, our data suggest that VEGFR2 might regulate proliferation and invasion in HNSCC cancer cells in vivo.
Chunpeng Yu,Jian Li,Qun Li,Shuai Chang,Yufeng Cao,Hui Jiang,Lingling Xie,Gang Fan,Song Wang 한국미생물학회 2022 The journal of microbiology Vol.60 No.11
Due to the evolutionary arms race between hosts and viruses, viruses must adapt to host translation systems to rapidly synthesize viral proteins. Highly expressed genes in hosts have a codon bias related to tRNA abundance, the primary RNA translation rate determinant. We calculated the relative synonymous codon usage (RSCU) of three hepatitis viruses (HAV, HBV, and HCV), SARS-CoV-2, 30 human tissues, and hepatocellular carcinoma (HCC). After comparing RSCU between viruses and human tissues, we calculated the codon adaptation index (CAI) of viral and human genes. HBV and HCV showed the highest correlations with HCC and the normal liver, while SARS-CoV-2 had the strongest association with lungs. In addition, based on HCC RSCU, the CAI of HBV and HCV genes was the highest. HBV and HCV preferentially adapt to the tRNA pool in HCC, facilitating viral RNA translation. After an initial trigger, rapid HBV/HCV translation and replication may change normal liver cells into HCC cells. Our findings reveal a novel perspective on virus-mediated oncogenesis.
Tao Li,Fengping An,Hui Teng,Qun Huang,Feng Zeng,Hong-bo Song 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.1
Resistant starches (RS) were prepared from purple yam by dual autoclaving-retrogradation (DAS), and pullulanase debranching treatment (PDS). DAS and PDS were then hydrolyzed by a-amylase and amyloglucosidase to obtain DAS.H and PDS.H. Differences in structural characteristics and in vitro digestibility among the four samples were investigated. The results showed that granules of RS had a rough surface and irregular shape. DAS had the lowest amylose content (29.52%), whereas PDS.H had the highest amylose content (41.96%). The order of crystallinity of the RS was: PDS.H (31.23%)[DAS.H (30.16%)[PDS (21.23%)[DAS (15.30%). Analysis by in vitro digestibility indicated a decreased hydrolysis index and glycemic index due to lower swelling power and water-binding capacity, and a well-ordered double helix structure and more crystallization in PDS.H than in the other RS samples. These results suggest that pullulanase debranching combined with a-amylase and amyloglucosidase hydrolysis may produce better RS with improved crystalline structure and higher digestion resistibility.
Zhong-Yuan Li,Hui-Qun Song,Jia Chen,Xing-Quan Zhu 대한기생충학열대의학회 2015 The Korean Journal of Parasitology Vol.53 No.3
Toxoplasma gondii is an opportunistic protozoan parasite that can infect almost all warm-blooded animals including humans with a worldwide distribution. Micronemes play an important role in invasion process of T. gondii, associated with the attachment, motility, and host cell recognition. In this research, sequence diversity in microneme protein 6 (MIC6) gene among 16 T. gondii isolates from different hosts and geographical regions and 1 reference strain was examined. The results showed that the sequence of all the examined T. gondii strains was 1,050 bp in length, and their A + T content was between 45.7% and 46.1%. Sequence analysis presented 33 nucleotide mutation positions (0-1.1%), resulting in 23 amino acid substitutions (0-2.3%) aligned with T. gondii RH strain. Moreover, T. gondii strains representing the 3 classical genotypes (Type I, II, and III) were separated into different clusters based on the locus of MIC6 using phylogenetic analyses by Bayesian inference (BI), maximum parsimony (MP), and maximum likelihood (ML), but T. gondii strains belonging to ToxoDB #9 were separated into different clusters. Our results suggested that MIC6 gene is not a suitable marker for T. gondii population genetic studies.
Li, Tao,An, Fengping,Teng, Hui,Huang, Qun,Zeng, Feng,Song, Hongbo 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.1
Resistant starches (RS) were prepared from purple yam by dual autoclaving-retrogradation (DAS), and pullulanase debranching treatment (PDS). DAS and PDS were then hydrolyzed by ${\alpha}-amylase$ and amyloglucosidase to obtain DAS.H and PDS.H. Differences in structural characteristics and in vitro digestibility among the four samples were investigated. The results showed that granules of RS had a rough surface and irregular shape. DAS had the lowest amylose content (29.52%), whereas PDS.H had the highest amylose content (41.96%). The order of crystallinity of the RS was: PDS.H (31.23%) > DAS.H (30.16%) > PDS (21.23%) > DAS (15.30%). Analysis by in vitro digestibility indicated a decreased hydrolysis index and glycemic index due to lower swelling power and water-binding capacity, and a well-ordered double helix structure and more crystallization in PDS.H than in the other RS samples. These results suggest that pullulanase debranching combined with ${\alpha}-amylase$ and amyloglucosidase hydrolysis may produce better RS with improved crystalline structure and higher digestion resistibility.