http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Utsumi, Hideo,Akira Hamada 嶺南大學校 環境問題硏究所 1993 環境硏究 Vol.12 No.2
In order to establish the method of bio-assay for the safety of drinking water, genotoxicity and cytotoxicity of micropollutants in water were studied. Genotoxicity was estimated with Ames Salmonella mutagenicity test, and cytotoxicity was evaluated with the cultured mammalian cells, HL-60, rat liver hepatocytes and mouse peritoneal macrophages. The dissolved substances in water were recovered from raw, chlorinated, and rapid-filtrated waters by adsorption with XAD-2. Some of the samples showed strong direct genotoxicity and/or cytotoxicities, but no correlation was observed between the results with the two bio-assays. The findings indicate that the combination of genotoxicity test with cytotoxicity one is recommended to secure the safety of drinking water.
Jin-Yi Han, Hideo Utsumi 충북대학교 동물의학연구소 2011 Journal of Biomedical and Translational Research Vol.12 No.1
We investigated free radical reactions in lung of living mice using an in vivo electron spin resonance (ESR) spectrometer and nitroxyl radical as a probe. When an aqueous solution of nitroxyl probe was trans-tracheally administered into lung of living mice, a sharp triplet signal was observed at the chest of the mice. The signal showed a gradual decrease with time, obeying first-order kinetics. Signal decay rates of carbamoyl-PROXYL and carboxy-2,2,6,6-tetramethyl-piperidine-N-oxyl were faster than those of CAT-1 and carboxy-PROXYL. The mechanism of signal decay may be attributed to (i) reaction with reactive oxygen species such as ·OH, (ii) transfer into blood circulation, or (iii) reduction by compounds continuously supplied. However, little is known about the clearance mechanism of the nitroxyl probe in lung. To evaluate the disappearance of the ESR signal of the nitroxyl probe in lung, in vivo ESR spectra in chest of mice was recorded after trans-tracheal administration of an aqueous high concentrate solution of nitroxyl probe. A broad signal from the chest was observed immediately after administration due to Heisenberg spin exchange interaction. A sharp triplet signal was superimposed on the broad signal and the appearance of a triplet signal was followed by disappearance of the broad one. Peak-to-peak line width of the sharp signal was almost the same as that after intravenous administration. A distinct signal was detected in blood collected 10 min after trans-tracheal administration of nitroxyl probe. The observations indicate the transfer from lung to blood circulation and its contribution to clearance of probe in lung. Appearance of a sharp signal in blood after trans-tracheal administration was dependent on the kind of nitroxyl probe, showing a different transfer rate from lung to blood.
Abe,Akemi,Utsumi,Hideo 嶺南大學校 環境問題硏究所 1995 環境硏究 Vol.15 No.1
Lethal action and the removal of molecular sulfur by copper treatment were investigated in the testing of mutagenicity for sediment samples. The results can be summarized as follows. (1) Sediment crude extracts showed lethal action on tester strains, TA98 and TA100. (2) The lethal was stronger in the sample from which larger amounts of extracts per unit weight were obtained. (3) The lethal action was reduced with the addition of the metabolizer S9 mix. (4) TA98 was more resistant than TA100 against the lethal substances in sediment extracts. (5) Removal of molecular sulfur raised the survival ratio of tester strains. (6) Lethal substances including molecular sulfur distributed in non-polar and slightly polar fraction, and medium or high polar fractions did not show lethal action. (7) Removal procedure of molecular sulfur was proper for applying on the fraction contained non polar and slightly polar compounds.