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Proteomic Analysis of the Neurotrophic Effect of Gelidium amansii in Primary Cultured Neurons
Md. Abdul Hannan,Md. Mohibbullah,홍용기,문일수 한국식품영양과학회 2017 Journal of medicinal food Vol.20 No.3
Gelidium amansii is an edible and economically important red alga consumed in South Eastern Asia. In previous studies, we reported that the ethanol extracts of G. amansii (GAE) has promising modulatory activity with respect to the morphological and functional maturation of hippocampal neurons in culture. In this study, we show that the chloroform (CHCl3) subfraction of GAE and the ethyl acetate (EtOAc) fraction dose-dependently promoted neurite outgrowth, and their effects were comparable with that of GAE. We further assessed in cultured cortical neurons, proteins differentially expressed in the presence/absence of the GAE, CHCl3 subfraction, and the EtOAc fraction by 2D-PAGE and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Proteomic data revealed that a number of proteins responsible for multiple cellular and biochemical functions vital for neuronal development and maturation were significantly upregulated in neurons treated with the GAE, CHCl3 subfraction, and the EtOAc fraction. Of the identified proteins, profilin 2a, septin 7, cdc42, protein phosphatase 2A, DA11, eukaryotic translation initiation factor 5A-1, and γ-enolase are known to play important roles in neuritogenesis and dendritic arborization. Immunofluorescence data demonstrate that GAE-treated hippocampal neurons showed greater intensity ratios in the expressions of the septin 7 and cdc42 compared to vehicle control, validating their proteomic profiles. Together these results suggest that the GAE/CHCl3 subfraction and EtOAc fraction promote neurite development by up or downregulating several key proteins.
A brown alga Sargassum fulvellum facilitates neuronal maturation and synaptogenesis.
Hannan, Md Abdul,Kang, Ji-Young,Hong, Yong-Ki,Lee, Hyunsook,Chowdhury, Muhammad Tanvir Hossain,Choi, Jae-Suk,Choi, In Soon,Moon, Il Soo Springer 2012 In vitro cellular & developmental biology Animal Vol.48 No.8
<P>Sargassum fulvellum (Turner) C. Agardh is an edible brown macroalgae having pharmacological importance. In previous reports, we described the screening of marine algae for their neuritogenic activity in developing hippocampal neurons and found that ethanol extract of S. fulvellum (SFE) possesses promising neurite-outgrowth-promoting activity. In this study, we evaluated whether the initial neurite promoting effect of SFE was followed on the further neuronal maturation and synapse formation. SFE exhibited dose-dependent effect on neurite maturation with an optimum concentration of 5?μg/mL. The initial neuronal differentiation is significantly promoted by SFE. Subsequently, compared with control culture, SFE increased the indices of axonal and dendritic developments such as the number and the length of primary processes, and branching frequencies. In addition to its effect on neurite development, SFE significantly increased the number of puncta for postsynaptic density-95, synaptic vesicle 2, and synapse (about 35%, 67%, and 125%, respectively, of control). Moreover, SFE dose-dependently protects neurons from naturally occurring death in normal culture condition. Taken together, our data demonstrate that SFE can promote neuronal maturation and synaptogenesis and support neuronal survival, suggesting the beneficial effect of this alga in nervous system.</P>
Md. Mohibbullah,Md. Abdul Hannan,박인식,문일수,홍용기 한국식품영양과학회 2016 Journal of medicinal food Vol.19 No.7
The edible red seaweed Gracilariopsis chorda (Holmes) Ohmi is known for its extensive medicinal benefits and its use as a food ingredient in Korea, Japan, and China. In a previous study, an ethanol extract of G. chorda (GCE) showed potential neuroprotective effects in cultured hippocampal neurons. In this study, we further examined the ability of GCE to promote neurite extension in primary rat hippocampal neurons. Neurons were stained with the lipophilic dye DiO or immunostained to visualize the neuronal morphology. The results indicated that GCE concentration-dependently increased neurite outgrowth, with an optimal concentration of 30 μg/mL. GCE significantly promoted early neuronal differentiation (i.e., polarity and process number) and enhanced axonal and dendritic arborization in a time-responsive manner. In addition, arachidonic acid, which was previously identified and quantified as a major neuroprotective component of GCE, significantly accelerated neurite outgrowth similar to GCE. Our findings suggest that G. chorda and its active component, arachidonic acid, may be useful for developing medicinal food or pharmaceuticals in the prevention and treatment of neurological disorders.
Maqueshudul Haque Bhuiyan, Mohammad,Mohibbullah, Md,Hannan, Md Abdul,Hong, Yong-Ki,Choi, Jae-Suk,Choi, In Soon,Moon, Il Soo Institute for Advanced Research in Asian Science a 2015 The American journal of Chinese medicine Vol.43 No.3
<P>Reductions in neurotrophic factors are implicated in synaptic dysfunction in the central nervous system, but exogenous neurotrophic factors with potential effects on neuritic regeneration and synaptic reconstruction could offer therapeutic and preventive strategies for treating memory-related neurological disorders. In an earlier effort to identify natural neurotrophic agents, we found that the ethanol extract of the edible marine alga Undaria pinnatifida (UPE) had promising effects on the neuritogenesis of cultured hippocampal neurons. Here, we further investigated the ability of UPE to promote spinogenesis and synaptogenesis in primary cultures of hippocampal neurons. It was found that UPE triggered significant increase in numbers of dendritic filopodia and spines, promoted the formation of excitatory and inhibitory synapses, and potentiated synaptic transmission by increasing the sizes of reserve vesicle pools at presynaptic terminals. These findings indicate a substantial role for UPE in the morphological and functional maturation of neurons and suggest that UPE is a possible therapeutic preventative measure and treatment for neurodegenerative diseases, such as those involving cognitive disorders and memory impairments.</P>
Repositioning Vitamin C as a Promising Option to Alleviate Complications associated with COVID-19
Farjana Mithila,Moni Akhi,Sohag Abdullah Al Mamun,Hasan Adeba,Hannan Md. Abdul,Hossain Md. Golzar,UDDIN MD JAMAL 대한감염학회 2020 Infection and Chemotherapy Vol.52 No.4
Vitamin C, also known as L-ascorbic acid, is an essential vitamin with pleiotropic functions, ranging from antioxidant to anti-microbial functions. Evidence suggests that vitamin C acts against inflammation, oxidative stress, autophagy chaos, and immune dysfunction. The ability to activate and enhance the immune system makes this versatile vitamin a prospective therapeutic agent amid the current situation of coronavirus disease 2019 (COVID-19). Being highly effective against the influenza virus, causing the common cold, vitamin C may also function against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and its associated complications. Severe infections need higher doses of the vitamin to compensate for the augmented inflammatory response and metabolic demand that commonly occur during COVID-19. Compelling evidence also suggests that a high dose of vitamin C (1.5 g/kg body weight) in inflammatory conditions can result in effective clinical outcomes and thus can be employed to combat COVID-19. However, further studies are crucial to delineate the mechanism underlying the action of vitamin C against COVID-19. The current review aims to reposition vitamin C as an alternative approach for alleviating COVID-19-associated complications.
HyunSook Lee,Ji Hye Lee,Min-Jin Kang,Md. Abdul Hannan,Il Soo Moon 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1
N-acetylglucosamine (GlcNAc) kinase (NAGK; EC 2.7.1.59) converts GlcNAc into GlcNAc-6-phosphate. Based on the proposed 3D structure, we produced 3 point mutant NAGKs that were expected to retain differential capacities for substrate binding and reaction velocity. The proteins were expressed in Escherichia coli, affinity-purified to homogeneity, and used for functional analysis. Among the mutants, conversion of Cys143, which does not make direct hydrogen bonds with GlcNAc to Ser (i.e., C143S) had the least affecton enzymatic activity. Conversion of Asn36, which plays a role in domain closure by making a hydrogen bond with GlcNAc to Ala (i.e., N36A) mildly reduced the enzyme activity. Conversion of Asp107, which makes hydrogen bonds with GlcNAc and acts as a proton acceptor to Ala (i.e., D107A), caused a total loss in the enzyme activity. The eGFP- or RFP (DsRed)-tagged mutant NAGKs increased the complexity of dendritic architecture when overexpressed in rat hippocampal neurons (DIV 5-9) with no statistical difference with wild-type NAGK. These results indicate that the upregulation of dendritic complex by NAGK is the enzyme’s non-canonical function.