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MEILING ZOU,HAN ZHU,PAN WANG,MINGLIANG DU,MING ZHANG,SHIYONG BAO 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.3
In this paper, epigallocatechin gallate (EGCG) was used as a green reductant both for thefabrication of soluble reduced graphene oxide (rGO) and the synthesis of Au nanoparticles/rGOnanocomposite. Fourier transform infrared (FTIR) spectra con¯rmed the e±cient removal of theoxygen-containing groups in graphene oxide (GO) through the reduction act of EGCG. Aunanoparticles (AuNPs) were anchored onto the rGO sheets by heating the mixed solution of rGOand chloroauric acid at 65?C using EGCG as reductant. Transmission electron microscopy(TEM), atomic force microscope (AFM) and X-ray photoelectron spectroscopy (XPS) wereemployed to characterize the resulting nanocomposite. Due to the chelating e®ect of polyhydroxyEGCG, AuNPs with diameters of ? 20 – 50 nm were stably decorated onto both sides of the rGOsheets. Because this reduction method avoids the use of toxic reagents, AuNPs/rGO nano-composite would be eco-friendly, and it might be useful not only for electronic devices but also forbiocompatible materials in the future applications.
SHIYONG BAO,HAN ZHU,PAN WANG,MEILING ZOU,MINGLIANG DU,MING ZHANG 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2013 NANO Vol.8 No.6
A facile and green route was introduced to synthesize Pt nanoparticles (PtNPs) immobilized on Cu2O octahedrons to form Cu2O–Pt hierarchical heterostructure. Transmission electron microscopy (TEM), field emission scanning electron microscopy (FE-SEM), high resolution transmission electron microscopy (HRTEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) were employed to study their morphology, chemical and crystallographic properties of the Cu2O–Pt hierarchical heterostructure. These novel Cu2O–Pt hierarchical heterostructures show fascinating degradations of methylene blue (MB), due to the suppressed electron/hole recombination phenomena and the efficient ability to capture the light.
Cun Hui,Siyan Wang,Mingliang Liu,Weichi Han,Ran Hai 한국강구조학회 2022 International Journal of Steel Structures Vol.22 No.4
In order to study the infl uence of stiff eners on the wind resistance performance of the aluminum panel curtain wall unit (APCU), two APCU specimens with diff erent structures were designed and fabricated. The deformations under positive and negative wind loads were studied. The defl ection, stress, and mode were analyzed by fi nite element software. The results indicate that the stiff eners and the aluminum panels work well with each other under positive and negative wind pressure. Adequately designed stiff eners can eff ectively improve the wind resistance of the APCU, reduce the deformation of the aluminum curtain wall, and make the stress distribution uniform. The shedding of aluminum welding nails has a signifi cant impact on the overall wind resistance performance of the component. The aluminum panels and the stiff eners reduce the wind resistance performance of the APCU when the aluminum panels and stiff eners are not fi rmly connected. The modal analysis points out the weak point of the specimens. The fi nite element calculations and analysis results are consistent with the actual measurement results. The research results can guide engineering applications. The research in this paper can provide guidance for wind resistance of aluminum plate, defl ection deformation of stiff ening rib, failure of aluminum plate under wind pressure and engineering research of aluminum curtain wall system under wind load.
Zhao Yongjie,Shang Shengwen,Song Ya,Li Tianyue,Han Mingliang,Qin Yuexuan,Wei Meili,Xi Jun,Tang Bikui 한국미생물학회 2022 The journal of microbiology Vol.60 No.11
Mycobacterium tuberculosis (M. tuberculosis) is a highly pathogenic intracellular pathogen that causes tuberculosis (TB), the leading cause of mortality from single infections. Redox homeostasis plays a very important role in the resistance of M. tuberculosis to antibiotic damage and various environmental stresses. The antioxidant sulforaphane (SFN) has been reported to exhibit anticancer activity and inhibit the growth of a variety of bacteria and fungi. Nonetheless, it remains unclear whether SFN exhibits anti-mycobacterial activity. Our results showed that the SFN against M. tuberculosis H37Ra exhibited bactericidal activity in a time and dose-dependent manner. The anti-tubercular activity of SFN was significantly correlated with bacterial reactive oxygen species (ROS) levels. In addition, SFN promoted the bactericidal effect of macrophages on intracellular bacteria in a dose-dependent manner, mediated by increasing intracellular mitochondrial ROS levels and decreasing cytoplasmic ROS levels. Taken together, our data revealed the previously unrecognized antimicrobial functions of SFN. Future studies focusing on the mechanism of SFN in macrophages against M. tuberculosis are essential for developing new host-directed therapeutic approaches against TB.
Antiviral effects of Bovine antimicrobial peptide against TGEV in vivo and in vitro
Xiuli Liang,Xiaojun Zhang,Kaiqi Lian,Xiuhua Tian,Mingliang Zhang,Shiqiong Wang,Cheng Chen,Cunxi Nie,Yun Pan,Fangfang Han,Zhanyong Wei,Wen-Ju Zhang 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.5
Background: In suckling piglets, transmissible gastroenteritis virus (TGEV) causes lethal diarrhea accompanied by high infection and mortality rates, leading to considerable economic losses. This study explored methods of preventing or inhibiting their production. Bovine antimicrobial peptide-13 (APB-13) has antibacterial, antiviral, and immune functions. Objectives: This study analyzed the efficacy of APB-13 against TGEV through in vivo and in vitro experiments. Methods: The effects of APB-13 toxicity and virus inhibition rate on swine testicular (ST) cells were detected using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT). The impact of APB-13 on virus replication was examined through the 50% tissue culture infective dose (TCID50). The mRNA and protein levels were investigated by real-time quantitative polymerase chain reaction and western blot (WB). Tissue sections were used to detect intestinal morphological development. Results: The safe and effective concentration range of APB-13 on ST cells ranged from 0 to 62.5 μg/mL, and the highest viral inhibitory rate of APB-13 was 74.1%. The log10TCID50 of 62.5 μg/mL APB-13 was 3.63 lower than that of the virus control. The mRNA and protein expression at 62.5 μg/mL APB-13 was significantly lower than that of the virus control at 24 hpi. Piglets in the APB-13 group showed significantly lower viral shedding than that in the virus control group, and the pathological tissue sections of the jejunum morphology revealed significant differences between the groups. Conclusions: APB-13 exhibited good antiviral effects on TGEV in vivo and in vitro.
Small molecules enable OCT4-mediated direct reprogramming into expandable human neural stem cells
Zhu, Saiyong,Ambasudhan, Rajesh,Sun, Woong,Kim, Hyun Jung,Talantova, Maria,Wang, Xiaojing,Zhang, Mingliang,Zhang, Yu,Laurent, Timothy,Parker, James,Kim, Han-Seop,Zaremba, Jeffrey D,Saleem, Sofiyan,San Springer Science and Business Media LLC 2014 Cell research Vol.24 No.1