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RISS 인기검색어
- 검색키워드
- 저자 : Haihong Han (검색결과 4 건)
- 무료
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Gang Han,Haihong Huang 한국자기학회 2020 Journal of Magnetics Vol.25 No.2
In this study, a multi-physics finite element model is adopted to investigate the effect of geometric discontinuity and stress concentration on the magnetic memory method (MMM). We propose a quadratic equation to fit the stress magnetization constitutive relation in the model. The relation can be used to predict the abnormality of the geometric discontinuity and the stress concentration. Simulation results show that the magnetization on the wall of the groove is the weakest and that on the bottom of the groove is the maximum. The free boundary condition releases the stress concentration of the defect. Although the magnetization induced by stress concentration is weaker than geometric discontinuity, the signal characteristics can still be used to evaluate the defect and the stress. This work proves that the MMM is a potential method for stress distribution assessment.
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Le Xue,Haihong Han,Han Fan,Piwu Li,Tengfei Wang,Jing Xiao,Ruiming Wang,Junqing Wang 한국생물공학회 2019 Biotechnology and Bioprocess Engineering Vol.24 No.5
To prevent cellulose loss during bio-pulping, two cellulase genes in Bacillus licheniformis 20085 were knocked out by homologous exchange technique. An engineering strain B. licheniformis 20085Δcelb was significantly compromised the ability to disintegrate filter paper compared to the original strain. After fermentation for 60 h, the CMCase activity value decreased from 1.86 to 0.50 U/mL, suggesting that the celb gene played an important role in the process of cellulose degradation in B. licheniformis 20085. We also assessed the ability of B. licheniformis 20085Δcelb to pulp bamboo. The yield of bamboo pulp was 51.91%, the kappa number was 11.26%, and 1.3% of cellulose was degraded after bio-pulping. Environmental Scanning Electron Microscope (ESEM) studies were also conducted to visualize the effect of these strains on bamboo cell walls. These results indicate that pulping with B. licheniformis 20085Δcelb can generate a higher yield and lower kappa number.
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DNA Microarray-Based Global Transcriptional Profiling of Yersinia pestis in Multicellularity
Jingfu Qiu,Zhaobiao Guo,Haihong Liu,Dongsheng Zhou,Yanping Han,Ruifu Yang 한국미생물학회 2008 The journal of microbiology Vol.46 No.5
Yersinia pestis, the causative agent of plague, has a feature of forming multicellular aggregates at liquid-air interface around the wall of glass tube. In this study, we employed the whole-genome DNA microarray of Y. pestis to investigate the global transcriptional profile in multicellularity compared with that in its planktonic growth. A total of 177 genes were differentially expressed in Y. pestis during early stage of multicellular formation; Seventy genes of them were up-regulated while 107 down-regulated. In addition to a large number of genes encoding unknown functions, most of the induced genes encode cell envelope and transport/binding proteins. The up-regulation of amino acid biosynthesis, the differentially altered genes that are involved in virulence, and the cold shock protein genes were for the first time reported to be associated with the multicellular formation. Our results revealed the global gene expression of Y. pestis were changed in the formation of multicellularity, providing insights into the molecular mechanism of multicellular behaviour, which need investigating further.
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SC35 promotes splicing of the C5-V6-C6 isoform of CD44 pre-mRNA
LOH, TIING JEN,MOON, HEEGYUM,CHO, SUNGHEE,JUNG, DA-WOON,HONG, SEONG-EUI,KIM, DO HAN,GREEN, MICHAEL R.,ZHENG, XUEXIU,ZHOU, JIANHUA,SHEN, HAIHONG D.A. Spandidos 2014 ONCOLOGY REPORTS Vol.31 No.1
<P>CD44 is a cell membrane glycoprotein that mediates the response of cells to their cellular microenvironment and regulates growth, survival, differentiation and motility. CD44 pre-mRNA contains 20 exons, 10 of which are alternatively spliced. Among the CD44 spliced variants, one of the V6 exon-containing isoforms, the V4–7 variant which contains variable exons 4, 5, 6 and 7, confers metastatic potential to non-metastatic cells. However, the splicing regulation of the V6 exon is not completely understood. SC35 is an arginine-serine rich protein that regulates alternative splicing of various pre-mRNAs. In the present study, we established a stable cell line which indicates inclusion or skipping of the V6 exon with the RFP or GFP signal. Using this stable cell line, we found that the V6 exon and flanking introns of CD44 pre-mRNA contained SC35 response elements that regulate V6 splicing. RT-PCR analyses of the endogenous CD44 splicing showed that SC35 promotes the production of the C5-V6-C6 isoform. shRNA knockdown of SC35 showed that reduced expression of SC35 decreased expression of the V6 exon-containing isoforms. Our results reveal a novel mechanism of CD44V6 splicing.</P>
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