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Anti-cancer Effect of Cordyceps Bassiana derived KTH-13
Ji Hye Kim,Woo Seok Yang,Han Gyung Kim,Eun ji Kim,Sung youl Hong,Jae Youl Cho 한국버섯학회 2014 버섯 Vol.18 No.2
In this study, we aimed to examine the cellular and molecular mechanisms of KTH-13 (4-isopropyl-2,6-bis(1-phenylethyl)phenol) which is derived from Cordyceps staphylindaecola in the cancer cells survival. The apoptotic effect of KTH-13 on various cancer cells, such as C6 glioma, MDA-MB-231 breast cancer, and A549 cells, was determined by MTT assay, and result showed that KTH-13 (0-100mM) dramatically inhibited the cancer cell survival. IC50 of KTH13 were 60.549, 53.512, >100, in C6, MDA-MB-231, and A549, respectively. DNA fragmentation result revealed that MDA-MB-231 cells treated with KTH-13 100mM undergoes apoptosis. To understand the action mechanism of KTH-13, the effect of KTH-13 on caspase which is key regulator of apoptosis was verified. The amount of cleaved capspase-3 and 7, executioner caspases, was increased by KTH-13 treatment, at time dependent (capspase-3 case) and dose dependent manner (caspase-7 case). And the cleavage of caspase-9 which is initiator caspase was also elevated in KTH-13 treated MDA-MB-231 cells showing time dependent manner. However, caspase-8 was not regulated by KTH-13, indicating KTH-13 specifically targets caspase-9 signal. As caspase-9 is closely associated with intrinsic pathway, the involvement of bcl-2 family was identified. Bax, pro-apoptotic molecule, was up-regulated whereas Bcl-2, anti-apoptotic protein, was down-regulated. And the Bax/Bcl-2 ratio was increased about 10 times. Then, the survival signal was also observed. The phosphorylation of Akt and p85 was diminished by KTH-13 treatment at 2,4,6 and 8 hour. Collectively, results suggest that KTH-13 induces cancer cells apoptosis via caspase3, 7, 8 and Bcl-2 family signaling pathway. And the Akt and p85 is also involved in KTH-13 action mechanism.
Maintenance Therapy of Facial Seborrheic Dermatitis with 0.1% Tacrolimus Ointment
( Hye One Kim ),( Yoon Seok Yang ),( Hyun Chang Ko ),( Gyung Moon Kim ),( Sang Hyun Cho ),( Young Joon Seo ),( Sang Wook Son ),( Jong Rok Lee ),( Joong Sun Lee ),( Sung Eun Chang ),( Jae We Che ),( Ch 대한피부과학회 2015 Annals of Dermatology Vol.27 No.5
Background: Topical calcineurin inhibitors (TCIs) have been successfully used to treat seborrheic dermatitis (SD) patients. Meanwhile, treatment of atopic dermatitis (AD) with lowdose, intermittent TCI has been proved to reduce disease flare-ups. This regimen is known as a maintenance treatment. Objective: The aim of this trial was to investigate the efficacy and tolerability of a maintenance treatment with tacrolimus ointment in patients with facial SD. Methods: During the initial stabilization period, patients with facial SD or AD applied 0.1% tacrolimus ointment twice daily for up to 4 weeks. Clinical measurements were evaluated on either in the whole face or on separate facial regions. When an investigator global assessment score 1 was achieved, the patient applied tacrolimus twice weekly for 20 weeks. We also compared our results with recent published data of placebo controlled study to allow an estimation of the placebo effect. Results: The time to the first relapse during phase II was similar in both groups otherwise significantly longer than the placebo group. The recurrence-free curves of two groups were not significantly different from each other; otherwise the curve of the placebo group was significantly different. There were no significant differences between the 2 groups in the number of DEs, and treatment days for disease exacerbations (DEs). The adverse event profile was also similar between the 2 groups. During the 20 weeks of treatment, the study population tolerated tacrolimus ointment well. Conclusion: The results of this study suggest that maintenance treatment with tacrolimus may be effective in preventing the occurrence of facial SD exacerbations. (Ann Dermatol 27(5) 523∼530, 2015)
Yang, Woo Seok,Ko, Jaeyoung,Kim, Eunji,Kim, Ji Hye,Park, Jae Gwang,Sung, Nak Yoon,Kim, Han Gyung,Yang, Sungjae,Rho, Ho Sik,Hong, Yong Deog,Shin, Song Seok,Cho, Jae Youl Hindawi Publishing Corporation 2014 Mediators of inflammation Vol.2014 No.-
<P>21-O-Angeloyltheasapogenol E3 (ATS-E3) is a triterpenoid saponin recently isolated from the seeds of the tea tree <I>Camellia sinensis</I> (L.) O. Kuntze. ATS-E3 has several beneficial properties including anti-inflammatory, antidiabetic, antiatherosclerotic, and anticancer effects. Unlike other phenolic compounds isolated from tea plants, there are no studies reporting the pharmacological action of ATS-E3. In this study, we therefore aimed to explore the cellular and molecular inhibitory activities of ATS-E3 in macrophage-mediated inflammatory responses. ATS-E3 remarkably diminished cellular responses of macrophages such as FITC-dextran-induced phagocytic uptake, sodium nitroprusside- (SNP-) induced radical generation, and LPS-induced nitric oxide (NO) production. Analysis of its molecular activity showed that this compound significantly suppressed the expression of inducible NO synthase (iNOS), nuclear translocation of nuclear factor- (NF-) <I>κ</I>B subunits (p50 and p65), phosphorylation of inhibitor of <I>κ</I>B kinase (IKK), and the enzyme activity of AKT1. Taken together, the novel triterpenoid saponin compound ATS-E3 contributes to the beneficial effects of tea plants by exerting anti-inflammatory and antioxidative activities in an AKT/IKK/NF-<I>κ</I>B-dependent manner.</P>
증례 : 호흡기 ; 객혈과 폐종괴로 내원한 amiodarone 유인성 폐독성 1예
정혜철 ( Hye Cheol Jeong ),이지현 ( Ji Hyun Lee ),정준구 ( Jun Gu Chung ),조양현 ( Yang Hyun Cho ),주세경 ( Sae Kyung Joo ),선한경 ( Han Gyung Seon ),김은경 ( Eun Kyung Kim ) 대한내과학회 2009 대한내과학회지 Vol.77 No.5S
Amiodarone은 부정맥의 치료에 효과적으로 사용되고 있지만 폐독성의 발생빈도가 약물 복용자의 1~15%로 보고되어 있어 사용에 주의를 요하는 약물이다. 종괴상 병변으로 amiodarone 유인성 폐독성이 나타난 예는 드물며 이는 폐의 악성 신생물, 결핵 등 타 질환과의 감별을 요하기 때문에 중요하다고 할 수 있다. 저자 등은 객혈로 내원한 환자에서 흉부 방사선 검사상 좌폐상엽의 종괴가 관찰되어 흉부 신생물로 오인될 수 있었던 amiodarone 유인성 폐독성의 1예를 경험하였으며 약물 중단 이후 객혈 및 종괴상 병변의 호전을 관찰하여 이에 보고하는 바이다. Amiodarone is an antiarrhythmic drug that has adverse effects involving the lungs and other organs. Amiodarone-induced pneu-monitis is a distinct, not uncommon form of drug-induced lung injury. By contrast, amiodarone-induced pulmonary toxicity presenting with hemoptysis and a lung mass is rare. We report the case of a 67-year-old woman with hemoptysis and a lung mass who had taken amiodarone for 6 years to control a ventricular arrhythmia. After drug withdrawal without any additional treatment, clinical and radiological improvement was observed. (Korean J Med 77:S1202-S1205, 2009)
C++ 프로그램의 이해도 증진을 위한 역공학 시각화 도구
문양선(Yang-Sun Moon),김재웅(Jae-Woong Kim),조혜경(Hye--Gyung Cho),유철중(Cheol-Jung Yoo),김용성(Yong-Sung Kim),장옥배(Ok-Bae Chang) 한국정보과학회 1995 정보과학회 컴퓨팅의 실제 논문지 Vol.1 No.2
이 논문은 캡술화, 상속성, 그리고 다형성이 객체 지향 프로그램의 분석과 이해를 어떠한 면에서 어렵게 하는지를 C++ 예제 프로그램을 통해 구체적으로 설명한 후, C++ 프로그램의 분석과 이해 증진을 위해 개발한 시각화 시스템을 소개한다. 이 시스템은 이미 개발된 시스템에 몇 가지 기능을 첨가한 것으로서 시스템의 주된 기능은 다음과 같다. (1) 프로그램의 복잡도를 자동적으로 측정한다. (2) 객체 지향 설계 및 프로그래밍에관한 지침을 보여준다. (3) 모듈(클래스와 함수)들의 원시 코드를 추적한다. (5) 메시지 전달 경로 그래프(함수 호출 경로 그래프)를 보여준다. (6) 모듈간의 관계 그래프를 보여준다. This paper explains concretly through C++ program in which respect encapsulation, inheritance and polymorphism make object-oriented programs difficult in analysing and understanding program, and introduces a visualization tool developed for the enhancement of analysis and understanding on C++ programs. The tool has been added some kinds of functions to our previous work. The main functions of the tool : (1) measure the complexity of a C++ program automatically, (2) introduce a guideline for the object-oriented design and programming, (3) trace the source code of modules( classes and functions), (4) display the path graph of message passing( function call path graph), (5) display the relationship graph between functions.
장은혜 ( Eun Hye Jang ),양경혜 ( Gyung Hye Yang ),이정미 ( Jeong Mi Lee ),정명애 ( Myung Ae Chung ),손진훈 ( Jin Hun Sohn ) 한국감성과학회 2009 감성과학 Vol.12 No.4
본 연구는 아동을 대상으로 기쁨, 슬픔, 분노, 스트레스와 무료함의 다섯 가지 정서를 유발시키는 정서프로토콜을 개발하고 이를 표준화하고자 실시되었다. 심상을 유발하는 이야기·음악·색깔·인형을 이용하여 각각의 정서를 유발시키는 상황을 구성하였다. 또한 정서 평가를 위하여 아동용 정서평가척도를 개발하였다. 아동은 다섯 가지의 정서 상황을 경험한 후, 자신의 정서 상태를 평가하였다. 정서 프로토콜에 대한 평가는 아동의 자기보고와 전문가의 행동 관찰 분석으로 이루어졌다. 연구 결과, 개발된 정서 프로토콜은 각각의 정서를 효과적으로 유발시킨 것으로 나타났다. 연령별·별 비교에서는, 남아보다는 여아가 무료함을 더 강하게 느꼈으며, 학령 이후의 아동은 학령이전의 아동보다 슬픔에 더 민감한 것으로 나타났다. The aim of this study was to develop a research protocol that provides standardized conditioning stimuli in effectively evoking emotion-specific responses in children. The protocol targets five specific emotions(i.e., happiness, sadness, anger, distress, and boredom) by introducing study participants to a combination of music, color, stories, and dolls. This research protocol also includes a self-report emotion assessment scale specially developed for this study to verify the types of emotion induced. Evaluation of the method entailed triangulation of the results from the subjects` self-reported emotional state corresponding to each conditioned stimulus as well as behavioral observations conducted by researchers. Findings suggest that this new protocol effectively evokes five emotions in correspondence to the way it was intended for appropriate emotion induction. Additionally, results showed that female children than male children are more likely to feel boredom; and school-aged children were more likely to react to sadness than pre-school children.
Kim, Ji Hye,Lee, Yunmi,Sung, Gi-Ho,Kim, Han Gyung,Jeong, Deok,Park, Jae Gwang,Baek, Kwang-Soo,Sung, Nak Yoon,Yang, Sungjae,Yoon, Deok Hyo,Lee, Sang Yeol,Kang, Hyojeung,Song, Changsik,Cho, Jae Han,Lee, Hindawi Publishing Corporation 2015 Evidence-based Complementary and Alternative Medic Vol.2015 No.-
<P>The <I>Cordyceps</I> species have been widely used for treating various cancer diseases. Although the Cordyceps species have been widely known as an alternative anticancer remedy, which compounds are responsible for their anticancer activity is not fully understood. In this study, therefore, we examined the anticancer activity of 5 isolated compounds derived from the butanol fraction (Cb-BF) of <I>Cordyceps bassiana</I>. For this purpose, several cancer cell lines such as C6 glioma, MDA-MB-231, and A549 cells were employed and details of anticancer mechanism were further investigated. Of 5 compounds isolated by activity-guided fractionation from BF of Cb-EE, KTH-13, and 4-isopropyl-2,6-bis(1-phenylethyl)phenol, Cb-BF was found to be the most potent antiproliferative inhibitor of C6 glioma and MDA-MB-231 cell growth. KTH-13 treatment increased DNA laddering, upregulated the level of Annexin V positive cells, and altered morphological changes of C6 glioma and MDA-MB-231 cells. In addition, KTH-13 increased the levels of caspase 3, caspase 7, and caspase 9 cleaved forms as well as the protein level of Bax but not Bcl-2. It was also found that the phosphorylation of AKT and p85/PI3K was also clearly reduced by KTH-13 exposure. Therefore, our results suggest KTH-13 can act as a potent antiproliferative and apoptosis-inducing component from <I>Cordyceps bassiana</I>, contributing to the anticancer activity of this mushroom.</P>