이동형 생체계측을 위한 임베디드 컴퓨팅

홍주현,진계환,박경순,조명찬,박길선,차은종,이태수 충북대학교 의과대학 충북대학교 의학연구소 2003 忠北醫大學術誌 Vol.13 No.2

연구목적: 초소형 정밀 기계 기술로 제작된 2차원 가속도 센서와 916 MH2 단거리 고주파 데이터 통신 기술을 응용하여 인체의 운동상태를 자동으로 감지하고 분류하는 기법을 제안 하고자 하였다. 재료 및 방법: 인체의 신호를 획득하기 위하여 미국 버클리대에서 제작한 무선 센서를 등 산용 조끼의 전면상단에 부착해서, 대상자의 흉골 부위에 위치하도록 하고, 앉은 상태에서 일어서거나 눕거나 하는 동작 및 그 역동작이 일어날 때, 가속도 센서에서 감지되는 4가지 종류의 파형 현화에 해당되는 데이터를 무선센서 네트웍을 통해 획득하였다. 결과: 획득한 신호를 주성분분석(Principal Component Analysis)과 지원벡터머신(Support Vector Machine)을 사용하여, 2차주성분까지를 도표화하고 클러스터링 함으로써, 4개의 영역으로 분할하는 것이 가능함을 말 수 있었다 결론: 소형 센서들이 작동되는 임베디드 컴퓨팅 환경에서 일상생활 중에 발생하는 인체의 4가지 동작을 자동으로 감지하고 분류하는 것이 가능함을 보였다. Purpose: New monitoring and classifying method of human motion contort was pro- posed by using 2-axial MEMS accelerometer and 916 MHz short-range data communica-tion technology. Materials and Methods: The wireless sensor of MICA mote was attached to a woman mountain vest and located close to the subject's sternum. During four types of sub-ject's motion, that is, sit-to-stand, stand-to-sit, sit-to-tying, and tying-to-sit, waveform changes of the accelerometer data were acquired by wireless sensor network. Results: The acquired signal was analyzed by Principal Component Analysis(PCA) and Support Vector Machine(SVM) technique. By clustering the first and second principal components, four regions corresponding to the four types of motion, were clearly segment-ed. Conclusion: Human motion context during daily life could be monitored and classified automatically by using small sensors in embedded computing environment

Effects of 3,3′,4,4′,5-pentachloro biphenyl (PCB126) on the Expression the Tight Junction Genes in Cultured Mouse Neonatal Testis

Gye Myeong-Chan,Fukuzawa Noriho Honda,Ohsako Seiichiro 한국발생생물학회 2003 한국발생생물학회 학술발표대회 Vol.2003 No.1

In an effort to uncover the spermatogenic impairment by the polychlorinated biphenyls (PCBs), the expression of tight junctions (TJs) genes important for the formation of the blood testis barrier (BTB) were examined following the 3,3',4,4',5-pentachloro biphenyl (PCB126) treatment in cultured neonatal testis in mice. At 4 days (D4) after 10 and 100 nM PCB126 treatment the expression of claudin-11 was significantly increased when compared with vehicle control. In contrast no difference in occludin and claudin-1 expression was found among the experimental group. On D8, 100 nM PCB126 significantly increased the expression of claudin-11 but not occludin and claudin-1. 1 uM PCB126 treatment significantly decreased expressions of occludin and ciaudin -1, suggesting the general toxic effect on the Sertoli cell. Because PCB126 does not alter the proliferative activity of spermatogenic cells and Sertoli cells in neonatal testis, it is likely that increase in the expression of claudin-11 by low dose of PCB126 may attribute to the alteration of the Sertoli cells differentiation in testis. It also emphasized that PCB126 might have differentially affected the transcription of TJ genes in Sertoli cells. In conclusion, this result suggests that the structure of TJ may be targeted by PCB126 in neonatal testis in mice and that co-PCB is potentially harmful to spermatogenesis by alteration of the development of BTB.

명지산 일대의 양서류

계명찬 ( Gye Myeong Chan ) 한국환경생물학회 2003 환경생물 : 환경생물학회지 Vol.21 No.2

경기도 명지산의 양서류 다양성을 조사하고 군집 구조 및 서식지 특성을 분석하였다. 2002월4월 2003년 3월에 거친 4회의 조사를 통해 양서류 2목 5과 7종이 발견되었으며 우점도는 무미야서류인 무당개구리가 전체 45.1%로 가장 높았고, 물두꺼비의 경우 12.8%를 차지하였다. 옴개구리, 북방산개구리는 각각 9.9%를 차지하였으며, 계곡산개구리와 참개구리는 1.4%를 차지하였다. 유미 양서류인 꼬리치레도룡뇽은 7.0%를 차지하였다. 꼬리치레도룡뇽과 물두꺼비의 출현빈도 및 출현장소는 서식지의 고도와 인간의 인위적 간섭에 크게 영항을 받는 것으로 나타났다. 출현확률에 따른 위협도 분석결과 이 지역에서 참개구리와 계곡산개구리가 서식밀도가 낮은 것으로 나타나 원인 분석과 함께 보호가 요청된다. 명지산 양서류의 종 풍부도(R^(`))는 0.98, 다양도(H^(`))는 2.12로 비교적 높은 값을 보인 반면 균등도(E^(`))는 0.76으로 비교적 낮은 값을 보이고 있어 양서류 군집의 구조가 복잡성을 보일 뿐 아니라 종의 구성이 비교적 풍부한 것으로 사료된다. 가평천 상류의 명지산 일대가 양서류 서식에 양호한 조건을 갖추고 있으며 양서류 서식지로서의 보전가치가 매우 큰 것으로 판단된다. Amphibian biodiversity, community structure and habitats were surveyed in the Mt. Myungji area from April 2002 to March 2003. During the survey period 2 orders, 4 families, 7 species of amphibians were observed. Bombina orientalis (DI =45.1%) was dominant and Bufo stqjnegeri (DI = 12.8%), Rana rugosa and Rana dybowskii (DI = 9.9% each) Rana nigromaculata and Rana huanrenensis (DI = 1.4% each), and Onycodactylus fisheri (DI = 7.0%) followed. Frequency of occurrence of 0. fisheri and B. stqjnegeri was largely different according to the altitude and human residence in their habitats. R. nigromaculata and R. huanrenensis showed low frequency of occurrence (< 10%) suggesting the decrease in habitation density in this area. The species richness (R`), general diversity (H`) and evenness (E`) of amphibians in this area were 0.98,2.12, and 0.76, respectively, suggesting relatively healthy condition of amphibian community in this area.

수환경 내 Estrogen 에스트로젠 활성 검출을 위한 누치 난황전구단백질 유전자 발현의 RT-PCR 시험법

계명찬 ( Gye Myeong Chan ) 한국하천호수학회 2004 생태와 환경 Vol.37 No.1

In an effort to develop the viomarker for monitoring the contamination of xenoestogen in the freshwater environment of Korea, reverse transcription-polymerase chain reaction (RT-PCR) analysis of vitellogenin(VTG) gene expression was optimized in Hemibarbus labeo. Based on the homology of the VTG cDNA sequences between the common carp and zebra fish, a set of PCR primers for VTG m RNA amplification for H. labeo was designed. VTG mRNA level in livers in livers from female and male fishes was analyzed by RT-PCR following single injection of 17 beta estradiol(E₂10㎎ ㎏^(-1)B.W.).As an internal control, beta actin mRNA was amplified. One ug of total liver RNA was subjected to RT-PCR. In female the amount of PCR product of VTG gradually icreased in the range from 16 to 34 cycles of amplification. On the contrary, in control male, PCR product first detected at 32 cycles of amplification and linearly increased up to 40 cycles of amplification. In E₂injected male liver,the VTG mRNA level was similar to that in the female. Taken together, this result suggests that liver of male H. labeo expresses minute amount of VTG mRNA which are 2^(-16)equivalent of female and that induction of VTG mRNA occurs in male liver after estrogen treatment. In conclusion, the optimized protocol for RT-PCR analysis of VTG mRNA expression in liver of male H. labeo will provide the environmental monitoring method for the xenoestrogen contamination in the rivers in Korea.

Proteomics를 이용한 내분비계장애물질인 nonylphenol에 노출된 무당개구리의 단백질 발현 비교 연구

김호승 ( Kim Ho Seung ),계명찬 ( Gye Myeong Chan ) 한국하천호수학회 2003 생태와 환경 Vol.36 No.3

Nonylphenol (NP), an well known aquatic contaminant, has been known to induce abnormalities in various aquatic animals. In an effort to develop proteome in the study of aquatic contamination of NP and its impact on the amphibia, protein changes in liver tissues of Korean red bellied frog, Bombina orientalis was investigated following the NP exposure. NP was administered intraperitoneally to male B. orientalis at 10 mg/kg body weight. At 48 to 96th after the treatment, the frog livers were sampled, and the protein fraction was separated using two dimensional gel electrophoresis (2D/E) and visualized with Coomassie brilluant blue staining. The 2D/E images of the tissue from the animals treated with NP showed marked changes of protein spots(about 20% of total protein spots). Analysis of the 50-60 separated spots allowed identification of the major protein changes in the overall pattern for the stressor (NP) by time (, 48 and 96h). At 48h after treatment, 8 spots were increased and 12 spots were reduced. Then, at 96h after treatment, 10 spots were increased and 8 spots were reduced. In total, approximately 29% of liver proteins showed the altered expression following the NP treatment. It is suggested that protein expression was repressed by blocking of certain metabolisms at 48 h and induced by the synthesis of new proteins for adaptation at 96 h following NP exposure. This application for 2D/E analysis may show promise in searching biomarkers for environmental proteomics in amphibians.

생쥐의 자궁내막에서 p27(kip1)과 p57(kip2)의 발현

김성태 ( Kim Seong Tae ),이성기 ( Lee Seong Gi ),계명찬 ( Gye Myeong Chan ) 대한산부인과학회 2004 Obstetrics & Gynecology Science Vol.47 No.3

목적 : 생쥐의 생리주기와 임신기간동안 자궁내막에서 CDK (cyclin-dependent kinase) inhibitor인 p27^(kip1)과 p57^(kip2) 유전자의 발현양상을 알아보고자 하였다. 연구 방법 : 생쥐의 생리주기별, 그리고 임신 1-6일의 자궁내막 조직에서 전체 RNA를 추출하여 semiquantitative RT-PCR 방법을 이용하여 p27^(kip1)과 p57^(kip2) 유전자의 전사양상을 조사하였고, 단백질을 추출하여 western blotting 방법을 이용하여 단백질 발현 양상을 연구하였다. 결과 : p27^(kip1)과 p57^(kip2) mRNA는 발정기와 발정후기보다는 발정전기와 발정말기에서 높은 발현양상을 나타내었다. 이에 비해 임신 5일의 p27^(kip1)을 제외하고는 임신 1일에서 임신 6일까지 비교적 높은 발현수준을 유지하였다. 또한 p57^(kip2) 단백질은 임신 1일에서 4일까지는 비교적 낮은 발현수준을 나타내다가 임신 5일과 6일에서는 유의한 증가양상을 나타내었다. 결론 : 본 연구결과로 p27^(kip1)과 p57^(kip2)는 생쥐의 생리주기와 착상을 위한 자궁내막의 분화에 중요한 역할을 할 것으로 사료되며, 특히 p57^(kip2)는 착상의 유지를 위한 자궁내막 분화에 핵심적인 역할을 할 것으로 사료된다. Objective : This study was to investigate the expression of CDK inhibitors, p27^(kip1) and p57^(kip2) in mouse endometrium during the estrus cycle and pregnant period. Methods : Total RNA and protein were extracted from endometrium of mouse sacrificed at diestrus, proestrus, estrus, and metestrus cycle, and at day 1-6 post-coitum (p.c.), then semi-quantitative RT-PCR and western blotting of p27^(kip1) and p57^(kip2) was carried out. Results : p27^(kip1) and p57^(kip2) mRNA was highly expressed in diestrus and proestrus stage than estrus and metestrus stage. In comparison with estrus cycle, p27^(kip1) and p57^(kip2) mRNA level was highly maintained in gestational endometrium (except p27^(kip1) of day 5 p.c). p57^(kip2) protein level was relatively low from day 1 p.c. to day 4 p.c. But it was significantly increased in day 5 p.c. and day 6 p.c. Conclusion : These results show that p27^(kip1) and p57^(kip2) may play a role in endometrial differentiation for regular estrus cycle and implantation, and especially p57^(kip2) may play an essential role in endometrial differentiation for maintenance of implantation.

생쥐의 태반에서 p27(kip1)과 p57(kip2)의 발현

김성태 ( Kim Seong Tae ),이성기 ( Lee Seong Gi ),김경진 ( Kim Gyeong Jin ),계명찬 ( Gye Myeong Chan ) 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.10

목적 : 임신한 생쥐의 태반 발달과정동안 CDK (cyclin-dependent kinase) inhibitor인 p27(kip1)과 p57(kip2) 유전자의 발현양상을 알아보고자 하였다. 연구 방법 : 임신 12일, 14일, 16일, 18일의 생쥐 태반 조직에서 전체 RNA를 추출하여 semi-quantitative RT-PCR 방법을 이용하여 p27(kip1)과 p57(kip2) 유전자의 전사양상을 조사하였고, 단백질을 추출하여 western bl Objective : This study was to investigate the expression of CDK inhibitors, p27(kip1) and p57(kip2) during the growth and differentiation of mouse placenta. Methods : Total RNA and protein were extracted from placenta of mouse sacrificed at day 12, 14, 16