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( Patel,Kuldeep J. ),( Saurabh Vig ),( G. Nareshkumar ),( G. Archana ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.11
Rhizosphere microorganisms possessing phytase activity are considered important for rendering phytate-phosphorus (P) available to plants. In the present study, the Citrobacter braakii phytase gene (appA) was overexpressed in rhizobacteria possessing plant growth promoting (PGP) traits, for increasing their potential as bioinoculants. AppA was cloned under the lac promoter in the broadhost- range expression vector pBBR1MCS-2. Transformation of the recombinant construct pCBappA resulted in high constitutive phytase activity in all of the eight rhizobacterial strains belonging to genera Pantoea, Citrobacter, Enterobacter, Pseudomonas (two strains), Rhizobium (two strains), and Ensifer that were studied. Transgenic rhizobacterial strains were found to display varying levels of phytase activity, ranging from 10-folds to 538-folds higher than the corresponding control strains. The transgenic derivative of Pseudomonas fluorescens CHA0, a well-characterized plant growth promoting rhizobacterium, showed the highest expression of phytase (~8 U/mg) activity in crude extracts. Although all transformants showed high phytase activity, rhizobacteria having the ability to secrete organic acid showed significantly higher release of P from Caphytate in buffered minimal media. AppA overexpressing rhizobacteria showed increased P content, and dry weight (shoot) or shoot/ root ratio of mung bean (Vigna radiata) plants, to different extents, when grown in semisolid agar (SSA) medium containing Na-phytate or Ca-phytate as the P sources. This is the first report of the overexpression of phytase in rhizobacterial strains and its exploitation for plant growth enhancement.
Synthesis, Characterization, and Dyeing Performance of Thiadiazole Derivatives
G. M. Malik,P. C. Patel,J. H. Tailor,S. S. Patel 한국섬유공학회 2018 Fibers and polymers Vol.19 No.8
N4-(5-methyl-1,3,4-thiadiazol-2-yl)thiazole-2,4-diamine derivatives have been synthesized by the reaction of 2- amino-5-methyl-1,3,4-thiadiazole and chloroaecetylchloride with suitable solvent, then cyclized with thiourea at reflux temperature in methanol to yield N4-(5-methyl-1,3,4-thiadiazol-2-yl)thiazole-2,4-diamine which was diazotized and coupled with various naphthalene acid couplers to give new series of acid dyes (AD1-AD13). All the compounds were characterized by their percentage yield, melting point, elemental analysis, UV spectra, IR spectra, and NMR spectra and dyeing performance on nylon fabric has been assessed.
Amino acid functionalized magnetite nanoparticles in saline solution
Patel, D.,Chang, Y.,Lee, G.H. Elsevier 2009 CURRENT APPLIED PHYSICS Vol.9 No.1
We report amino acid functionalized magnetite nanoparticles with particle diameters, ranging from 3 to 10nm. We synthesized them in one-step in a saline solution. The saturation magnetizations (M<SUB>s</SUB>s) were determined to be 57.98 and 45.20emu/g at 300K for l-glutamic acid and l-lysine functionalized magnetite nanoparticles, respectively. Especially, l-lysine functionalized magnetite nanoparticles showed an excellent colloidal stability of >a year or so. This excellent colloidal stability, a high saturation magnetization, and a nearly monodisperse particle diameter of the l-lysine functionalized magnetite nanoparticles will be useful for biomedical applications.
Mrunali R. Patel,Rashmin B. Patel,Jolly R. Parikh,Bharat G. Patel 한국약제학회 2016 Journal of Pharmaceutical Investigation Vol.46 No.1
The objective of this study was to develop and evaluate a novel microemulsion (ME) formulation containing tazarotene for targeted topical therapy of acne. Microemulsions (MEs) were formulated by spontaneous microemulsification method using 12 % Isopropyl myristate, mixed emulsifiers 15 % Labrasol-Cremophor-RH 40 (1:1), 15 % Capmul MCM and 58 % water (w/w) as an external phase. All plain and tazarotene loaded MEs were clear and showed physicochemical parameters for desired topical delivery and stability. The permeation profile of tazarotene through rat skin from selected ME formulation exhibited highest skin uptake. The microscopic observations indicated that the optimized ME had no significant effect on the microscopic structure of the skin and epithelial cells appeared mostly unchanged. The surface epithelium lining and the granular cellular structure of the skin were totally intact. The developed ME may be a potential drug delivery vehicle for targeted topical delivery of tazarotene in the treatment of acne.
Singh, N.,Sivaramaiah, G.,Rao, J.L.,Watanabe, S.,Rao, T.K. Gundu,Patel, R.V.,Singh, Vijay Elsevier 2017 Journal of luminescence Vol.188 No.-
<P><B>Abstract</B></P> <P>Gadolinium activated UV-emitting ZrO<SUB>2</SUB> phosphor has been prepared by combustion synthesis. The prepared phosphor was characterized using X-ray diffraction, scanning electron microscopy and energy dispersive X-ray techniques. The optical absorption spectrum in the 200−500nm region and excitation spectrum corresponding to the emission at 314nm for ZrO<SUB>2</SUB>:Gd<SUP>3+</SUP> phosphor has been recorded. Gd<SUP>3+</SUP> doped ZrO<SUB>2</SUB> displays electron spin resonance signals with effective g values at <I>g</I>≈1.9, 2.4 and 4.1. Low field signals are attributed to Gd<SUP>3+</SUP> ion located in distorted surroundings experiencing a relatively strong crystal field due to the proximity of charge compensator oxygen vacancies.</P>
Niraj A. Shah,Laxit K. Bhatt,Rajesh J. Patel,Tushar M. Patel,Nayankumar V. Patel,Harshida G. Trivedi,Nilam R. Patel,Jitendra H. Patel,Satish D. Patel,Rajesh S. Sundar,Mukul R. Jain 한국실험동물학회 2022 Laboratory Animal Research Vol.38 No.4
Background: Nonhuman primates are used for research purposes such as studying diseases and drug discovery and development programs. Various clinical pathology parameters are used as biomarkers of disease conditions in biomedical research. Detailed reports of these parameters are not available for Indian-origin rhesus macaques. To meet the increasing need for information, we conducted this study on 121 adult Indian rhesus macaques (57 wild-sourced and 64 inhouse animals, aged 3–7 years). A total of 18 hematology and 18 biochemistry parameters were evaluated and reported in this study. Data from these parameters were statistically evaluated for significance amongst inhouse and wild-born animals and for differences amongst sexes. The reference range was calculated according to C28-A3 guidelines for reporting reference intervals of clinical laboratory parameters. Results: Source of the animals and sex appeared to have statistically significant effects on reference values and range. Wild-born animals reported higher WBC, platelets, neutrophils, RBC, hemoglobin, HCT, MCV, and total protein values in comparison to inhouse monkeys. Sex-based differences were observed for parameters such as RBCs, hemoglobin, HCT, creatinine, calcium, phosphorus, albumin, and total protein amongst others. Conclusions: Through this study, we have established a comprehensive data set of reference values and intervals for certain hematological and biochemical parameters which will help researchers in planning, conducting, and interpreting various aspects of biomedical research employing Indian-origin rhesus monkeys.
Molecular insights into the role of genetic determinants of congenital hypothyroidism
Kollati, Yedukondalu,Akella, Radha Rama Devi,Naushad, Shaik Mohammad,Patel, Rajesh K.,Reddy, G. Bhanuprakash,Dirisala, Vijaya R. Korea Genome Organization 2021 Genomics & informatics Vol.19 No.3
In our previous studies, we have demonstrated the association of certain variants of the thyroid-stimulating hormone receptor (TSHR), thyroid peroxidase (TPO), and thyroglobulin (TG) genes with congenital hypothyroidism. Herein, we explored the mechanistic basis for this association using different in silico tools. The mRNA 3'-untranslated region (3'-UTR) plays key roles in gene expression at the post-transcriptional level. In TSHR variants (rs2268477, rs7144481, and rs17630128), the binding affinity of microRNAs (miRs) (hsa-miR-154-5p, hsa-miR-376a-2-5p, hsa-miR-3935, hsa-miR-4280, and hsa-miR-6858-3p) to the 3'-UTR is disrupted, affecting post-transcriptional gene regulation. TPO and TG are the two key proteins necessary for the biosynthesis of thyroid hormones in the presence of iodide and H<sub>2</sub>O<sub>2</sub>. Reduced stability of these proteins leads to aberrant biosynthesis of thyroid hormones. Compared to the wild-type TPO protein, the p.S398T variant was found to exhibit less stability and significant rearrangements of intra-atomic bonds affecting the stoichiometry and substrate binding (binding energies, ΔG of wild-type vs. mutant: -15 vs. -13.8 kcal/mol; and dissociation constant, K<sub>d</sub> of wild-type vs. mutant: 7.2E<sup>-12</sup> vs. 7.0E<sup>-11</sup> M). The missense mutations p.G653D and p.R1999W on the TG protein showed altered ΔG(0.24 kcal/mol and 0.79 kcal/mol, respectively). In conclusion, an in silico analysis of TSHR genetic variants in the 3'-UTR showed that they alter the binding affinities of different miRs. The TPO protein structure and mutant protein complex (p.S398T) are less stable, with potentially deleterious effects. A structural and energy analysis showed that TG mutations (p.G653D and p.R1999W) reduce the stability of the TG protein and affect its structure-functional relationship.
Survey of Inositol in Infant Formula
Patel, A.,Ditiatkovski, M.,Kennedy, L.,Oglobline, A.,Choi, N.,Richardson, G. Korean Society for Mass Spectrometry 2016 Mass spectrometry letters Vol.7 No.1
Results of free and bound myo-inositol in infant formula (IF) are presented. Inositol was analyzed by HILIC ultra-performance liquid chromatography coupled with mass spectrometer. The levels of free myo-inositol in 27 Australian and 4 EU originated IF samples were 300-600 mg/kg of powder or 1.6-3.1 mg/100 kJ. The amount of bound inositol in lipid fraction of IF was, on average, 10% of free myo-inositol.