Effect of Cattle Breeds on Milk Composition and Technological Characteristics in China

Yang, T.X.,Li, H.,Wang, F.,Liu, X.L.,Li, Q.Y. Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.6

Cattle breeds have a striking effect on milk, including milk composition and technological characteristics. This study aims to compare milk composition, acidification activity, viscosity, milk dispersion system stability and casein molecular weight among three buffalo breeds in China. The technological characteristics of milk produced by three cattle breeds of river buffalo (Murrah), crossbreed 1st generation ($F_1$), crossbreed multiple generation ($F_H$, $H{\geq}3$) buffaloes were investigated. Cattle breeds showed evident effect on milk protein, fat and total solids content, but little effect on most of buffalo casein molecular weight. Milk fat, protein content and the viscosity of buffalo milk from river buffalo were lower than those of $F_1$ and $F_H$, so was the buffer capacity. The viscosity was negatively correlated to temperature and concentration. Results of stability coefficient showed that milk dispersion system had the best dynamic stability characteristics under pH 6.6 and 6 times dilution, while zeta potential of Murrah milk was slightly higher than that of hybrid offspring ($F_1$, $F_H$). SDS-PAGE results showed that buffalo ${\alpha}_s$-casein had a slightly faster mobility than standard ${\alpha}_s$-casein; while buffalo ${\beta}$-casein showed a slightly slower mobility than standard ${\beta}$-casein. There is no clear differences in molecular weight of ${\alpha}_s$-, ${\beta}$-, and ${\kappa}$-casein among Murrah, $F_1$ and $F_H$.

Effect of Dietary Antioxidant and Energy Density on Performance and Anti-oxidative Status of Transition Cows

Wang, Y.M.,Wang, J.H.,Wang, C.,Wang, J.K.,Chen, B.,Liu, J.X.,Cao, H.,Guo, F.C. Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.10

This study was conducted to evaluate the effect of dietary antioxidant and energy density on performance and antioxidative status in transition cows. Forty cows were randomly allocated to 4 dietary treatments in a $2{\times}2$ factorial design. High or low energy density diets (1.43 or 1.28 Mcal $NE_L$/kg DM, respectively) were formulated with or without antioxidant (AOX, a dry granular blend of ethoxyquin and tertiary-butylhydroquinone; 0 or 5 g/cow per d). These diets were fed to cows for 21 days pre-partum. During the post-partum period, all cows were fed the same lactation diets, and AOX treatment followed as for the pre-partum period. Feeding a high energy diet depressed the DMI, milk yield, and 4% fat-corrected milk (FCM) of cows. However, AOX inclusion in the diet improved the milk and 4% FCM yields. There was an interaction of energy density by AOX on milk protein, milk fat and total solids contents. Feeding a high energy diet pre-partum increased plasma glucose and ${\beta}$-hydroxybutyrate, whereas dietary AOX decreased plasma ${\beta}$-hydroxybutyrate value during the transition period. There were also interactions between time and treatment for plasma glutathione peroxidase activity and malondialdehyde content during the study. Cows fed high energy diets pre-partum had higher plasma glutathione peroxidase activity 3 days prior to parturition, compared with those on low energy diets. Inclusion of AOX in diets decreased plasma glutathione peroxidase activity in cows 3 and 10 days pre-partum. Addition of AOX significantly decreased malondialdehyde values at calving. Energy density induced marginal changes in fatty acid composition in the erythrocyte membrane 3 days post-partum, while AOX only significantly increased cis-9, trans-11 conjugated linoleic acid composition. The increase in fluidity of the erythrocyte membrane was only observed in the high energy treatment. It is suggested that a diet containing high energy density pre-partum may negatively affect the anti-oxidative status, DMI and subsequent performance. Addition of AOX may improve the anti-oxidative status and reduce plasma ${\beta}$-hydroxybutyrate, eventually resulting in improved lactation performance; the response to AOX addition was more pronounced on the high energy diet.

Genetic polymorphism in pvmdr1 and pvcrt-o genes in relation to in vitro drug susceptibility of Plasmodium vivax isolates from malaria-endemic countries

Lu, F.,Lim, C.S.,Nam, D.H.,Kim, K.,Lin, K.,Kim, T.S.,Lee, H.W.,Chen, J.H.,Wang, Y.,Sattabongkot, J.,Han, E.T. Verlag für Recht und Gesellschaft ; Elsevier 2011 Acta Tropica Vol.117 No.2

Treatment failure of chloroquine for Plasmodium vivax infection has increased in endemic countries. However, the molecular mechanisms for resistance and in vitro susceptibility of P. vivax to chloroquine remain elusive. We investigated the prevalence of mutations in the pvmdr1 and pvcrt-o genes, and the copy number of the pvmdr1 gene in isolates from the Republic of Korea (ROK), Thailand, the Union of Myanmar (Myanmar), and Papua New Guinea (PNG). We also measured in vitro susceptibility of Korean isolates to antimalarial drugs. The pvmdr1 analysis showed that mutations at amino acid position Y976F of pvmdr1 were found in isolates from Thailand (17.9%), Myanmar (13.3%), and PNG (100%), but none from the ROK, and mutation at position F1076L was present in isolates from the ROK (100%), Thailand (60.7%), and Myanmar (46.7%). One copy of the pvmdr1 gene was observed in most isolates and double copy numbers of the gene were observed in two Thai isolates. In the exons of the pvcrt-o gene that were sequenced, a K10 insertion was present in isolates from Thailand (56.0%) and Myanmar (46.2%), and the wild type was found in all Korean isolates. The results suggest that gene polymorphisms and copy number variation was observed in isolates of P. vivax from Southeast Asian countries. In Korean isolates polymorphism as limited to the F1076L variant, and no isolates with high level of resistance were found by in vitro susceptibility determinations. Moreover, our results provide a baseline for future prospective drug studies in malaria-endemic areas.

Molecular Characterization and Expression Pattern of Gene IGFBP-5 in the Cashmere Goat (Capra hircus)

Wang, X.J.,Shi, J.J.,Yang, J.F.,Liang, Y.,Wang, Y.F.,Wu, M.L.,Li, S.Y.,Guo, X.D.,Wang, Z.G.,Liu, D.J. Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.5

Insulin-like growth factor-binding protein-5 (IGFBP-5) is one of the six members of IGFBP family, important for cell growth, apoptosis and other IGF-stimulated signaling pathways. In order to explore the significance of IGFBP-5 in cells of the Inner Mongolian Cashmere goat (Capra hircus), IGFBP-5 gene complementary DNA (cDNA) was amplified by reverse transcription polymerase chain reaction (RT-PCR) from the animal's fetal fibroblasts and tissue-specific expression analysis was performed by semi-quantitative RT-PCR. The gene is 816 base pairs (bp) in length and includes the complete open reading frame, encoding 271 amino acids (GenBank accession number JF720883). The full cDNA nucleotide sequence has a 99% identity with sheep, 98% with cattle and 95% with human. The amino acids sequence shares identity with 99%, 99% and 99%, respectively. The bioinformatics analysis showed that IGFBP-5 has an insulin growth factor-binding protein homologues (IB) domain and a thyroglobulin type-1 (TY) domain, four protein kinase C phosphorylation sites, five casein kinase II phosphorylation sites, three prenyl group binding sites (CaaX box). The IGFBP-5 gene was expressed in all the tested tissues including testis, brain, liver, lung, mammary gland, spleen, and kidney, suggesting that IGFBP-5 plays an important role in goat cells.

《春香传》在五十年代中国的译介与传播

金ॗf;善(Jin, Chang-Shan), 王萌(Wang, Meng) 조선대학교 국제문화연구원 2012 국제문화연구 Vol.5 No.2

한반도 고전문학의 대표작 <춘향전>은 세계 속에 널리 알려진 지명도가 높은 작품의 하나이다. <춘향전>은 작품 그 자체의 독특한 특성과 1950년대 중국과 북한 간의 독특한 사회 역사적 환경으로 인하여 소설, 희곡 등 장르로 번역 개편 되여 광범위하게 수용 전파되면서 독특한 양상을 보여주었다. 1950년대 중국에서의 창극 <춘향전>과 소설 <춘향전>의 번역 전파는 당시 중국과 북한의 국민들간의 친선적 문화 공감대를 형성하는데 일조하였을 뿐만 아니라 중한 문학교류사의 중요한 한 페이지를 장식하였다.

Genetic Diversity of Chinese Indigenous Pig Breeds in Shandong Province Using Microsatellite Markers

Wang, J.Y.,Guo, J.F.,Zhang, Q.,Hu, H.M.,Lin, H.C.,Wang, Cheng,Zhang, Yin,Wu, Y. Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.1

To investigate the genetic diversity of six Chinese indigenous pig breeds in Shandong province (Laiwu Black, Dapulian Black, Licha Black, Yantai Black, Yimeng Black and Wulian Black), explain their genetic relationship and assess their integrity and degree of admixture with three Western commercial breeds (Landrace, Yorkshire and Duroc), 303 individuals from these breeds were genotyped for 26 microsatellite markers. In general, high genetic diversity (observed heterozygosity ranging from 0.5495 to 0.7746) and large breed differentiation ($F_{ST}$ = 0.188) were observed. The indigenous pig breeds in Shandong exhibited consistently higher levels of genetic diversity than the three Western breeds. However, compared with the Western breeds, which have an $F_{ST}$ value of 0.252, the indigenous breeds in Shandong have smaller $F_{ST}$ value of 0.145. The analysis of breed relationship indicated that the six indigenous breeds are classified into two groups. One includes four breeds, Licha, Yantai, Yimeng and Wulian, which have experienced large gene introgression of the Western breeds through progressive crossbreeding as well as gene flow among themselves. The other includes Laiwu and Dapulian, which are less influenced by the Western breeds and other indigenous breeds in Shandong in the recent past. The results show that some measures must be taken to effectively protect these indigenous pig breeds in Shandong.

Anti-VEGF therapy in mRCC: differences between Asian and non-Asian patients

Wang, Y,Choueiri, T K,Lee, J-L,Tan, M-H,Rha, S Y,North, S A,Kollmannsberger, C K,McDermott, D F,Heng, D Y C Nature Publishing Group 2014 The British journal of cancer Vol.110 No.6

<P><B>Background:</B></P><P>Several reports suggest that vascular endothelial growth factor (VEGF)-targeted therapy in metastatic renal cell carcinoma (mRCC) may be more toxic in Asian <I>vs</I> non-Asian populations. Comparative efficacy of these agents with respect to ethnicity is not well characterised.</P><P><B>Methods:</B></P><P>A multicentre, retrospective, cohort study using Asian and non-Asian centres which collected data on ethnicity, dose reductions and outcomes using the International mRCC Database Consortium.</P><P><B>Results:</B></P><P>This study included 1024 (464 Asian, 560 non-Asian) patients with a 29.4 months median follow-up. The percentage of dose modifications/reductions between non-Asians and Asians was similar (55% <I>vs</I> 61% <I>P</I>=0.1197). When adjusted for risk groups, there was no difference in overall or progression-free survival between non-Asians and Asians. Patients with dose reductions due to toxicity had longer treatment durations and overall survival than those who did not in both non-Asian (10.6 <I>vs</I> 5.0 months, <I>P</I><0.0001; 22.6 <I>vs</I> 16.1 months, <I>P</I>=0.0016, respectively) and Asian populations (8.9 <I>vs</I> 5.4 months, <I>P</I>=0.0028; 28.0 <I>vs</I> 18.7 months, <I>P</I>=0.0069, respectively).</P><P><B>Conclusions:</B></P><P>Adjusting for risk groups, there appears to be no difference in outcome between Asian <I>vs</I> non-Asian patients with mRCC treated with VEGF-targeted therapy. Judicious dose reductions may allow for better outcomes in both populations due to longer treatment durations, but direct comparisons are needed.</P>

A numerical investigation into the long-term behaviors of Fukushima-derived 137Cs in the ocean

Zhao, C.,Wang, G.,Qiao, F.,Wang, G.,Jung, K. T.,Xia, C. Springer Science + Business Media 2015 Acta oceanologica Sinica Vol.34 No.12

Impact of structural disorder on the magnetic ordering and magnetocaloric response of amorphous Gd-based microwires

Biswas, Anis,Yu, Y. Y.,Bingham, N. S.,Wang, H.,Qin, F. X.,Sun, J. F.,Yu, S. C.,Franco, V.,Srikanth, H.,Phan, M. H. American Institute of Physics 2014 JOURNAL OF APPLIED PHYSICS - Vol.115 No.17

<P>We have studied the impact of structural disorder on the magnetic ordering and magnetocaloric response of amorphous Gd68Ni32 and Gd53Al24Co20Zr3 microwires. We find that the presence of structural disorder significantly broadens the paramagnetic to ferromagnetic (PM-FM) transition and the temperature-dependent magnetic entropy change, while the nature of the second-order magnetic transition and long-range ferromagnetic order are not essentially affected by this effect. The large magnetic moment of Gd and the presence of the long-range ferromagnetic order are believed to result in a large magnetic entropy change, which together with the broadening of the PM-FM transition due to structural disorder contribute to a large refrigerant capacity. The excellent magnetocaloric properties of the amorphous microwires make them very promising candidates for active magnetic refrigeration. (C) 2014 AIP Publishing LLC.</P>

Assembling Mn:ZnSe quantum dots-siRNA nanoplexes for gene silencing in tumor cells

Wang, Y.,Yang, C.,Hu, R.,Toh, H.,Liu, X.,Lin, G.,Yin, F.,Yoon, H.,Yong, K. T. Royal Society of Chemistry 2015 Biomaterials Science Vol.3 No.1