Dissipation pattern and risk quotients assessment of amisulbrom in Korean melon cultivated in plastic house conditions

Kabir, Md. Humayun,Abd El-Aty, A. M.,Rahman, Md. Musfiqur,Chung, Hyung Suk,Lee, Han Sol,Park, Sun-Hyang,Hacımü,ftü,of,lu, Fazil,Chang, Byung-Joon,Shin, Ho-Chul,Shim, Jae-Han Springer-Verlag 2017 Environmental monitoring and assessment Vol.189 No.6

A quick and effective methodology for analyzing dinotefuran and its highly polar metabolites in plum using liquid chromatography-tandem mass spectrometry

Rahman, Md. Musfiqur,Abd El-Aty, A.M.,Kabir, Md. Humayun,Chung, Hyung Suk,Lee, Han Sol,Hacımü,ftü,of,lu, Fazil,Jeong, Ji Hoon,Chang, Byung-Joon,Shin, Ho-Chul,Shim, Jae-Han Elsevier 2018 Food chemistry Vol.239 No.-

<P><B>Abstract</B></P> <P>A simple and effective method was developed for analyzing dinotefuran and its three metabolites (MNG, UF, and DN) in plum using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Due to the polarity and high water miscibility, dinotefuran and some of its metabolites (especially DN) have some limitations to be extracted with acetonitrile and salt following the “QuEChERS” sample preparation methodology. Alternatively, the samples were extracted with methanol, and purified with dispersive-solid phase extraction procedure (d-SPE) using primary secondary amine (PSA) and C<SUB>18</SUB> sorbents after filtration, and mass up. Due to the suppression effect originated from plum matrix, matrix-matched calibration curves, which provided good linearity with coefficient of determination (<I>R<SUP>2</SUP> </I>)≥0.998, were used for quantification of all analytes. Blank plum samples fortified with 2 spiking levels (10×LOQ and 50×LOQ) yielded satisfactory recoveries for all tested analytes in the range of 83.01 to 110.18% with relative standard deviation (RSD)≤8.91. The method was successfully applied to field-incurred plum samples and dinotefuran and all metabolites were positively detected and quantified. In conclusion, we suggest that the method can be expanded to polar compounds having solvent and partitioning problems in any of the versions of QuEChERS.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A simultaneous method was developed for dinotefuran and its highly polar metabolites in plum. </LI> <LI> Mass-up was introduced instead of salting out for exact quantitation. </LI> <LI> Internal standard is not needed for the newly developed methodology. </LI> <LI> The method can be used either as it is or with slight modification in other matrices. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Uptake of the veterinary antibiotics chlortetracycline, enrofloxacin, and sulphathiazole from soil by radish

Chung, Hyung Suk,Lee, Young-Jun,Rahman, Md. Musfiqur,Abd El-Aty, A.M.,Lee, Han Sol,Kabir, Md. Humayun,Kim, Sung Woo,Park, Byung-Jun,Kim, Jang-Eok,Hacımü,ftü,of,lu, Fazil,Nahar, Nilufar,Sh Elsevier BV 2017 Science of the Total Environment Vol.605 No.-

<P><B>Abstract</B></P> <P>Veterinary antibiotics are available for uptake by the plants through sources such as manure, irrigation, and atmospheric interaction. The present study was conducted to estimate the half-lives of three veterinary antibiotics, chlortetracycline (CTC), enrofloxacin (ENR), and sulphathiazole (STZ), in soil and experimentally explore their uptake from contaminated soil to radish roots and leaves. Samples were extracted using a modified citrate-buffered version of the quick, easy, cheap, effective, rugged, and safe “QuEChERS” method followed by liquid chromatography coupled with tandem mass spectrometric analysis (LC–MS/MS) in the positive ion mode. Good linearity was observed for the three tested antibiotics in soil and plants (roots and leaves) with high coefficients of determination (<I>R</I> <SUP>2</SUP> ≥0.9922). The average recovery rates at two spiking levels with three replicates per level ranged between 77.1 and 114.8%, with a relative standard deviation (RSD)≤19.9% for all tested drugs. In a batch incubation experiment (<I>in vitro</I> study), the half-lives of CTC, ENR, and STZ ranged from 2.0–6.1, 2.2–4.5, and 1.1–2.2days, respectively. Under greenhouse conditions, the half-lives of the three target antibiotics in soil with and without radishes were 2.5–6.9 and 2.7–7.4; 4.7–16.7 and 10.3–14.6; and 4.4–4.9 and 2.5–2.8days, respectively. Trace amounts of the target antibiotics (CTC, ENR, and STZ) were taken up from soil <I>via</I> roots and entered the leaves of radishes. The concentration of CTC was lower than 2.73%, ENR was 0.08–3.90%, and <1.64% STZ was uptaken. In conclusion, the concentrations of the tested antibiotics decreased with time and consequently lower residues were observed in the radishes. The rapid degradation of the tested antibiotics in the present study might have only little impact on soil microorganisms, fauna, and plants.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A method to estimate the half-life of veterinary antibiotics in soil was developed. </LI> <LI> Veterinary antibiotic uptake rate by radish from contaminated soil was determined. </LI> <LI> The half-lives of antibiotics in soil with and without radishes did not differ. </LI> <LI> Trace antibiotic residue levels moved to roots and leaves from contaminated soil. </LI> <LI> We elucidate residual antibiotic transplantation and antibiotic tolerance in soil. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

고랭지 배추 재배지에서 사탕무씨스트선충의 물과 토양을 통한 포장 간 분산

권오경(Oh-Gyeong Kwon),신진희(Jin-Hee Shin),F. Md. Kabir,이재국(Jae-Kook Lee),이동운(DongWoon Lee) 한국원예학회 2016 원예과학기술지 Vol.34 No.1

사탕무씨스트선충(Heterodera schachtii)은 전세계적으로 십자화과 작물에 심각한 경제적 손실을 유발시키는 선충으로 2011년 우리나라의 고랭지 채소 재배지에 최초 발생이 확인되었다. 고랭지 배추 재배지에 사탕무씨스트선충에 의한 피해가 증가하고 있는 실정이나 피해 확산에 대한 실제적 원인 규명이 없어 본 연구에서는 고랭지 배추 재배지에서 작업화와 작업차량, 물을 통한 사탕무씨스트선충의 확산 가능성을 구명하기 위하여 수행하였다. 사탕무씨스트 선충 감염지에서 작업 할 경우 신발에 묻은 흙에 사탕무씨스트선충이 존재하였는데 부착 된 씨스트선충의 수는 지역별로 차이가 있었다. 사탕무씨스트선충에 감염된 배추 밭 농로를 주행 한 차량의 바퀴에서도 사탕무씨스트 선충의 부착이 확인 되었다. 고랭지 배추 재배지에서 배추 수확시 사용하는 굴삭기의 궤도에서는 41kg의 흙이 부착되었으며 장거리 수송용 5톤 트럭의 바퀴 네 개에서도 223.7g의 흙이 채취되어 수확 작업 시 다량의 사탕무시스트선충이 부착될 수 있음을 확인 할 수 있었다. 강우 시 사탕무씨스트선충 감염 포장에서 작업할 경우를 가정하여 관주량별(2, 4, 8Lㆍm<SUP>-2</SUP>)에 따른 작업화 부착 흙 양과 사탕무씨스트선충 부착량을 조사한 결과 관주량이 많을수록 장화에 부착되는 흙의 양은 많았으나 사탕무씨스트선충의 밀도는 차이가 없었다. 강우 시, 사탕무시스트선충 감염지에서는 유거수를 통해 사탕무씨스트 선충이 이동하였다. 우리나라 고랭지 배추 재배지에서 신규 도입 선충인 사탕무씨스트선충은 작업자의 작업화와 수확 작업에 이용되는 굴삭기와 차량의 바퀴 및 물을 통해 확산이 이루어 질 수 있음을 구명하였다. 따라서 사탕무씨스트선충 감염지역에서는 작업자의 개인 위생과 작업 차량에 대한 토양 제거를 통해 감염 확산을 막아야 할 것으로 판단된다. Sugar beet cyst nematode, Heterodera schachtii , causes serious economic losses worldwide in Brassicaceae crops. In 2011, this nematode was first found in highland vegetable cultivation areas in Korea, and thereafter spread to the surrounding healthy Chinese cabbage fields. However, little has been documented on the biological and ecological characteristics of the sugar beet nematode in highland vegetable cultivation areas. In this study the dispersal of the sugar beet cyst nematode was examined, focusing on spreading through soil and/or water infested with the nematode. When farmers with work boots trampled on Chinese cabbage fields for 10 minutes, the number of cysts recovered from the soil attached to the working differed depending on the research sites. Under irrigation conditions of 2, 4, and 8 liters of water per m², the amounts of soils attached on the bottom of the work boots and the number of healthy cysts in the soils increased significantly with the increase in soil moisture, although the total number of cysts, eggs, and juveniles did not increase significantly. After driving on the farm road adjacent to cabbage fields infested with the sugar beet cyst nematode, cysts were also recovered from the soil attached to the vehicle’s tires, suggesting that the sugar beet cyst nematode can spread to new fields through soil carried by vehicles and by farmers. An excavator and a motor truck could deliver 41 kg and 224 g, respectively, of soil on the shovel shoes and the wheels to other locations during the Chinese cabbage harvest, suggesting that farming equipment and transportation vehicles may be vital means by which the cyst nematode spreads to noninfested fields in the highland area of Korea. Runoff water also contained cysts, whose amounts increased after water irrigation onto the ridges to simulate rainy conditions, with no significant difference in the number of cysts with increasing amounts of irrigation water. All of these results confirmed that the sugar beet cyst nematode spreads through soil attached to work boots, harvesting tools, and transportation vehicles, especially during the harvest time, and through runoff water on rainy days. These observations suggest that personal sanitization and cleaning of working tools and vehicles are one of the most important means to prevent the dispersal of the sugar beet cyst nematode in fields.

온도와 뿌리 추출물이 사탕무씨스트선충, Heterodera schachtii의 부화와 발육에 미치는 영향

( F. Md. Kabir ),신진희 ( Jin Hee Shin ),권오경 ( Oh Gyeong Kwon ),이동운 ( Dong Woon Lee ) 한국토양동물학회 2015 한국토양동물학회지 Vol.19 No.1

Sugar beet cyst nematode, Heterodera schachtii, has recently been identified as a severe pest of Chinese cabbage field in the highland area of Korea. In this experiment, we tried to find out the effect of temperature and Chinese cabbage root extract in egg hatching as well as egg development of the nematode. Five different temperature conditions, 10, 15, 20, 25 and 30℃, with growth chamber were used to find the suitable hatching temperature where 25℃ showed the maximum egg hatching rate. However, 10 and 15℃ showed negligible hatching rate. In contrast, 20, 30 and 40 days-aged-Chinese cabbage root extracts were used for hatching in 25℃ climate chamber, where 20 days extract showed the highest hatching rate and 40 days presented the lowest egg hatching effect. To justify furthermore, egg development stages test carried out with 20 and 25℃ temperatures, where the egg development stage perform faster in 20 than 25℃.