http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Eun Hee Soh(蘇恩嬉),Young Am Chae(蔡永岩) 한국육종학회 1993 한국육종학회지 Vol.25 No.2
This experiment was carried out to obtain basic information for in vitro production of indigo via callus cultures in Polygonum tinctorium. The results indicated that callus was induced well from leaf disc on B5 medium containing 1㎎/ℓ of 2,4-D. Callus grown well on B5 medium which containing 19g/ℓ of yeast extract and 2㎎/ℓ of 2,4-D under dark condition. Sucrose(2%) as carbon source and nitrate nitrogen as nitrogen source were good for callus growth. When phosphate was added twice much to the basic medium, callus growth was increased. Indigo synthesis in callus cells was confirmed through wave length scanning and TLC analysis.
보관용기 및 저장조건에 따른 고추 및 배추종자의 10년간 발아율 추이
소은희(Eun Hee Soh),이우문(Woo Moon Lee),박기웅(Kee woong Park),최근진(Keun jin Choi),윤무경(Moo Kyoung Yoon) 한국원예학회 2014 원예과학기술지 Vol.32 No.6
종자 저장 시 보관조건은 종자의 활력유지에 중요한 영향을 미치며, 급격한 종자활력의 저하는 발아율저하의 중요 요인이 된다. 본 연구의 목적은 주요 채소작물 중 고추(Capsicum annuum L.) 및 배추(Brassica rapa L. ssp. pekinensis) 종자의 저장조건(저장온도 및 보관용기)에 따른 10년 동안의 발아율추이를 조사 분석함으로써 저장기간에 따른 두 종자의 활력유지를 위한 효율적 저장조건을 규명하고자 하였다. 고추 및 배추종자를 각각 진공 알루미늄 호일 봉투, 폴리에틸렌(PET)병 및 종이봉투에 실리카겔과 함께 밀봉하여 상온, 15℃ 및 5℃ 등 세 가지 온도조건 하에서 10년간 보관하면서 6개월마다 발아율검정을 수행하였다. 고추 및 배추종자는 15℃ 및 5℃에서는 보관용기에 상관없이 6.5년까지 저장하기 전의 활력을 그대로 유지하였으나 상온/종이봉투 조건하에서는 각각 4년째 및 5년째에 활력을 잃었다. 상온조건일지라도 진공 알루미늄 봉투에 보관 시 저장 6년까지 보관전의 활력을 유지하였고 특히 고추종자의 경우 저장기간 10년이 경과하여도 90% 이상의 발아율을 나타냈다. 본 연구결과에 의하면 종자저장시설(장기 및 중기)이 없더라도 고추종자의 경우 상온/진공 알루미늄 봉투, 배추종자의 경우 5℃/진공 알루미늄 호일 봉투에 보관한다면 10년 이상 90% 이상의 발아율을 유지할 수 있을 것으로 사료되며 특히 활력유지 점검을 위한 모니터링 기간을 늦춰 발아검정을 위한 시료낭비를 줄일 수 있을 것으로 판단된다. Seed viability is affected by storage conditions and rapid loss of viability in storage is the major cause for low germination. This study was carried out to examine the effect of packaging materials and storage temperature on seed germination rate over 10 years in two species (Capsicum annuum L. and Brassica rapa L. ssp. pekinensis) and determine effective storage conditions for maintaining seed viability. Seeds were packaged in aluminum poly pouches under vacuum, polyethylene bottles, and paper bags containing silica gel and stored under one of two controlled conditions (15℃, RH 40% or 5℃, RH 30%) or at ambient condition. Seed germination was recorded at 6-month intervals for 10 years. The seeds of both species showed no decline in viability until 6.5 years at 15 or 5℃, irrespective of packaging materials. However, under ambient conditions, the seeds of chili pepper and Chinese cabbage in paper bags lost viability after 4 and 5 years, respectively. By contrast, seeds of both species in vacuum-aluminum poly pouches exhibited a 99% germination rate after 6 years under ambient conditions. Pepper seeds in the vacuum-aluminum poly pouches maintained a 93% germination rate after 10 years in ambient conditions. These results indicated that a special seed storage facility for maintaining viability of chili pepper and Chinese cabbage seed might not be essential and seed testing would not be necessary for 10 years, if chili pepper and Chinese cabbage seeds were packed in ambient/vacuum-aluminum poly pouches or 5℃/vacuum-aluminum poly pouches.
국내에서 수집된 국화 품종에 대한 SSR 분자표지 Database 구축
심은조(Eun-Jo Shim),허은정(Eun-Jung Heo),윤무경(Moo-Kyoung Yoon),소은희(Eun-Hee Soh),홍지화(Jee-Hwa Hong) 한국육종학회 2015 한국육종학회지 Vol.47 No.4
Chrysanthemum (Dendranthema grandiflourm Kitamura) is a member of the Asteraceae and one of the important horticultural crops. The objective of this study was to construct a DNA profile database for identification of chrysanthemum varieties using simple sequence repeat (SSR) markers. In order to select SSR markers for the variety identification, we screened 587 SSR primers using 20 varieties. Among them, 27 SSR markers showed polymorphism. We finally selected 14 SSR markers showing peak clearance, high polymorphism and reproducibility in 20 varieties. In conclusion, DNA profile database for 147 chrysanthemum varieties were constructed by 14 SSR markers. A total of 79 SSR alleles were detected and three to ten alleles were detected with an average of 5.6 alleles per locus. The polymorphism information content value ranged 0.287 ~ 0.785 with an average of 0.598. Genetic relationship revealed that genetic distance of 147 varieties ranged from 0.44 to 1.00. The 143 varieties among 147 varieties were distinguished by 14 SSR markers but the 2 varieties developed by mutation breeding and natural variation were not distinguished from original varieties. These constructed SSR profile database will be useful for the selection of similar varieties for candidate variety and for solving problem relating to seed dispute and infringement of plant breeder’s right.
국내에서 수집된 자두의 품종식별을 위한 SSR Profile 데이터베이스 구축
홍지화(Jee-Hwa Hong),심은조(Eun-Jo Shim),박원흠(Won-Heum Park),소은희(Eun-Hee Soh) 한국육종학회 2015 한국육종학회지 Vol.47 No.2
This study was conducted to construct a DNA marker database for 38 plum varieties collected in Korea using simple sequence repeat (SSR) markers. A set of 61 SSR primer pairs was tested to select polymorphic SSR markers between 8 varieties. Among the 61 primer pairs, 21 showed polymorphism, reproducibility and easy scoring. The genetic relationship between the 21 SSR markers and 38 varieties was analyzed. A total of 210 polymorphic amplified fragments were obtained with the 21 SSR markers. Three to seventeen SSR alleles were detected for each locus, with an average of 10.0 alleles per locus. Average polymorphism information content (PIC) was 0.758, with a range from 0.549 to 0.870. A total of 210 SSR marker loci were used to calculate Jaccard’s distance coefficients for cluster analysis by an unweighted pair-group method with arithmetical average (UPGMA). The genetic distance ranged from 0.06 to 1.00 in 38 varieties. Out of 38 plum varieties, 32 were identified using the 21 SSR markers. Therefore, these SSR markers may be employed to complement distinctness, uniformity, and stability (DUS) tests or as potential tools to solve seed disputes regarding plums.
박찬웅(Chan-Woong Park),최근진(Keun-Jin Choi),소은희(Eun-Hee Soh),고희종(Hee-Jong Koh) 한국육종학회 2015 한국육종학회지 Vol.47 No.3
Community Plant Variety Rights (CPVR) system was introduced in 1995 in European Union. This right guarantees the exclusive exploitation rights for a plant variety and is effective in 28 EU member states through a single application to the Community Plant Variety Office (CPVO). The legal basis for the CPVR system is found in the Council Regulation (EC) No 2100/94 (the Basic Regulation). The scope of protection in the Basic Regulation is extended in the same way in UPOV 1991 convention. However, the protection of harvested material is not sufficiently well-defined in the Basic Regulation resulting in uncertainties and loopholes in the CPVR. By exceptions to the breeder’s right provision, farmers may save seed for some species provided that they pay an appropriate royalty to rights’ holder which is lower than that of equivalent certified seed. CPVR holderscurrently find it difficult to obtain royalties for farm saved seed (FSS) use, in part due to the difficulty in requesting information from farmers. The provision for ‘Essentially Derived Varieties’ (EDVs) helps to protect against plagiarism of plant varieties that are too similar to one another. However, there is no standardized protocol or threshold developed by CPVO to determine EDVs. The CPVR law provides a harmonized intellectual property regime for plant varieties at EU level, but enforcement varies widely in practice in Member States. And in some cases dispute resolution mechanisms are not easily accessible. This is considered to be one of the biggest problems inhibiting an effective EU-wide plant variety rights system.
박찬웅(Chan-Woong Park),최근진(Keun-Jin Choi),소은희(Eun-Hee Soh),고희종(Hee-Jong Koh) 한국육종학회 2016 한국육종학회지 Vol.48 No.1
Eighteen years have passed since Korea introduced plant variety protection (PVP) system. Korea is being considered as one of the countries which introduced PVP system successfully. However, there have been lots of changes in circumstances surrounding PVP system during this period. Regarding future direction of PVP system in Korea, firstly the function and role of three organizations which now independently operate PVP system need to be reorganized to improve efficiency in PVP operation dealing with global issues. Secondly, authorities need to devise more user-oriented application form and process. This is because breeders feel some difficulties in preparing application documents. Thirdly, Korea has to create sound environments which guarantee effective enforcement of breeders’ rights and secure reliability of the system against infringement. Regarding decision of infringement, a reasonable threshold should be set up to decide whether certain varieties are different from protected varieties or not using both growing test and DNA test. For essentially derived varieties (EDV), authorities need to establish a reasonable threshold to decide whether there is an essential derivation or not. In addition, to prevent dispute between PVP holders and farmers regarding the use of farm saved seeds in the future, clarification of farm saved seed article in legislation is necessary. Lastly, there might be some contradiction between PVP and Nagoya protocol in disclosure of origin, prior informed consent, benefit sharing, etc. In advance of enactment of domestic ABS law, authority needs to study impact of Nagoya protocol on PVP system to minimize confusion and damage on breeders.
토성에 따른 시호의 생육 및 Saikosaponin 함량
성낙술(Nak Sul Seong),김관수(Kwan Su Kim),소은희(Eun Hee Soh),채영암(Young Am Chae) 한국약용작물학회 1994 한국약용작물학회지 Vol.2 No.3
Bupleurum falcatum was cultivated in plots of different soil textures, which were sandy loam(SL), loam(L), and clay loam(CL). The growth characters of shoot and root parts in SL and L plots were better than CL one, and root yield was the most excellent in SL one. But the contents of total saikosaponins and methanol extract in its roots were the highest in CL plot. In any soil textures, one and two year old plants showed almost the same results in growth characters and saikosaponins contents. The growth and root yield of two year old plants were higher than those of one year old ones, but the contents of methanol extract and saikosaponins were lower. Plant characters were highly negative-correlated with the contents of saikosaponins and methanol extract.