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        Histopathologic Biomarker Response of Clam, Ruditapes decussates, to Organophosphorous Pesticides Reldan and Roundup: A Laboratory Study

        El-Shenawy, Nahla S.,Moawad, Tarak I.S.,Mohallal, Mahmoud E.,Abdel-Nabi, Ismail M.,Taha, Inas A. 한국해양학회 2009 Ocean science journal Vol.44 No.1

        Clam Ruditapes decussates was used as a biomonitoring organism to measure the potential impact of pollution. The present study investigated histological alterations of R. decussates following a long-term exposure to a sublethal-level of pesticides Reldan and Roundup. The clams were taken to the laboratory and kept for days to adjust to a continuous closed flow system with quality water control. After the adjustment period, clams were exposed either to sublethal concentrations of Reldan (0.6 $\mu$l $L^{-1}$) or Roundup (1.1 $\mu$l $L^{-1}$) for 90 days. Clams were taken to determine the histological damage at 30, 60 and 90 days. Most histological alterations were highly localized in the glandular cells of the gills, mantle tissues and digestive gland; secretions of glandular cells were found to be increased by increasing the time of exposure. Histopathological results showed the activation of resistance mechanisms that allowed the clams to survive under sublethal stresses. Thus, histological changes on localized tissues were sensitive and they have positive correlation to the time of exposure to the pesticides, suggesting that they may serve as biomarkers for Reldan and Roundup exposures.

      • KCI등재

        Histopathologic Biomarker Response of Clam, Ruditapes decussates, to Organophosphorous Pesticides Reldan and Roundup: A Laboratory Study

        Nahla S. El-Shenawy,Tarak I. S. Moawad,Mahmoud E. Mohallal,Ismail M. Abdel-Nabi,Inas A. Taha 한국해양과학기술원 2009 Ocean science journal Vol.44 No.1

        Clam Ruditapes decussates was used as a biomonitoring organism to measure the potential impact of pollution. The present study investigated histological alterations of R. decussates following a long-term exposure to a sublethal-level of pesticides Reldan and Roundup. The clams were taken to the laboratory and kept for days to adjust to a continuous closed flow system with quality water control. After the adjustment period, clams were exposed either to sublethal concentrations of Reldan (0.6 μl L−1) or Roundup (1.1 μl L−1) for 90 days. Clams were taken to determine the histological damage at 30, 60 and 90 days. Most histological alterations were highly localized in the glandular cells of the gills, mantle tissues and digestive gland; secretions of glandular cells were found to be increased by increasing the time of exposure. Histopathological results showed the activation of resistance mechanisms that allowed the clams to survive under sublethal stresses. Thus, histological changes on localized tissues were sensitive and they have positive correlation to the time of exposure to the pesticides, suggesting that they may serve as biomarkers for Reldan and Roundup exposures. Clam Ruditapes decussates was used as a biomonitoring organism to measure the potential impact of pollution. The present study investigated histological alterations of R. decussates following a long-term exposure to a sublethal-level of pesticides Reldan and Roundup. The clams were taken to the laboratory and kept for days to adjust to a continuous closed flow system with quality water control. After the adjustment period, clams were exposed either to sublethal concentrations of Reldan (0.6 μl L−1) or Roundup (1.1 μl L−1) for 90 days. Clams were taken to determine the histological damage at 30, 60 and 90 days. Most histological alterations were highly localized in the glandular cells of the gills, mantle tissues and digestive gland; secretions of glandular cells were found to be increased by increasing the time of exposure. Histopathological results showed the activation of resistance mechanisms that allowed the clams to survive under sublethal stresses. Thus, histological changes on localized tissues were sensitive and they have positive correlation to the time of exposure to the pesticides, suggesting that they may serve as biomarkers for Reldan and Roundup exposures.

      • KCI등재

        Comparing the Passive Sampler and Biomonitoring of Organic Pollutants in Water: A Laboratory Study

        Nahla S. El-Shenawy,Richard Greenwood,Ismail M. Abdel-Nabi,Zohour I. Nabil 한국해양과학기술원 2009 Ocean science journal Vol.44 No.2

        Passive sampling could provide the solution to problems associated with costly and time consuming sampling programmes and biomonitoring. Mussels (Mytilus edulis) and Chemcatcher passive sampler were simultaneously analyzed for sequestered pesticides and polychlorinated biphenyls (PCBs) by gas chromatography with mass spectrometry (GC/MSD). The Chemcatcher passive sampler based on a solid phase extraction disc with two types of diffusion-limiting membranes (polyethelene and polysulphone), were also compared. Diuron, atrazine, irgarol and lindane were accumulated in the polysulphone passive sampler in greater concentration than in the mussels or in the other passive samplers with polyethelene limiting-membrane. Mussels can accumulate in their tissue high concentration of non polar compounds such as PCB 52, dieldren and PCB 153; more than the passive sampler with polysulphone membrane. The device with polyethelene limiting membrane has high affinity capacity to concentrate high amount of phenenthrene, dieldrin, PCB 153 and PCB 52, so it acts as a sampler for non-polar compounds. The highest uptake rate of hydrophobic compounds by Chemcatcher was observed for analytes with log octanol-water partition coefficient (KOW) between 4.5 and 7.5. Laboratorybased studies using passive samplers to assess the potential for bioaccumulation could provide robust and reliable information at relatively low cost compared to biomonitoring data. Laboratory data obtained using passive samplers could be related to accumulation under field conditions where field assessments are required. Passive sampling could provide the solution to problems associated with costly and time consuming sampling programmes and biomonitoring. Mussels (Mytilus edulis) and Chemcatcher passive sampler were simultaneously analyzed for sequestered pesticides and polychlorinated biphenyls (PCBs) by gas chromatography with mass spectrometry (GC/MSD). The Chemcatcher passive sampler based on a solid phase extraction disc with two types of diffusion-limiting membranes (polyethelene and polysulphone), were also compared. Diuron, atrazine, irgarol and lindane were accumulated in the polysulphone passive sampler in greater concentration than in the mussels or in the other passive samplers with polyethelene limiting-membrane. Mussels can accumulate in their tissue high concentration of non polar compounds such as PCB 52, dieldren and PCB 153; more than the passive sampler with polysulphone membrane. The device with polyethelene limiting membrane has high affinity capacity to concentrate high amount of phenenthrene, dieldrin, PCB 153 and PCB 52, so it acts as a sampler for non-polar compounds. The highest uptake rate of hydrophobic compounds by Chemcatcher was observed for analytes with log octanol-water partition coefficient (KOW) between 4.5 and 7.5. Laboratorybased studies using passive samplers to assess the potential for bioaccumulation could provide robust and reliable information at relatively low cost compared to biomonitoring data. Laboratory data obtained using passive samplers could be related to accumulation under field conditions where field assessments are required.

      • SCIESCOPUSKCI등재

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