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COCCIDIOSIS CONTROL IN THE EUROPEAN UNION. CURRENT SITUATION AND FUTURE CHALLENGES
Marc Pagès,M. Dardi,M. de Gussem,J. Rubio,R. March,M. Shirley,D. Blake,E. del Cacho 한국가금학회 2012 한국가금학회 심포지움 Vol.2012 No.5
Avian coccidiosis is one of the most important diseases affecting the intensive poultry industry worldwide. Despite its outstanding importance and well-known relation with other factors that may influence the health status of the birds and its commercial performance, few studies are available on the distribution of Eimeria species in the field. In Europe the studies available until the present date are, Czech Republic(Kučera, 1990), France(Williams, 1996), UK(Chapman, 1982, Shirley 1995, Shirley 1997, Williams 2006, Eckert 1995), Norway(Haug, 2008)and Sweden(Thebo, 1998). Most of the published studies have been focused on identifying the seven species of Eimeria which affect Gallus gallus in a European country. However, the only survey that has been done exclusively with samples from broiler farms of Norway is Haug, 2008. In the present study, litter samples obtained in broiler farms from Spain, Belgium, Italy and France were evaluated for the presence of Eimeria species. The evaluation was done using a polymerase chain reaction(PCR) developed at IAH to specifically detect E.acervulina, E.maxima, E.mitis, E.praecox and E.tenella. Together with this molecular tool, oocyst counts and a morphometric evaluation test were also used to analyse the samples. Results obtained provide interesting data on the prevalence of species which affect the broiler birds in Europe as well as the percentage of farms with presence of each species. Together with prevalence data, rotation programs(anticoccidials-vaccines) appear to be of paramount importance to upgrade the flock performances. Data obtained in farms from the Netherlands confirms the advantage of using rotation programs with vaccines composed of Eimeria strains sensitive to anticoccidials.
Development and characterization of mouse monoclonal antibodies reactive with chicken TL1A
Lee, S.H.,Lillehoj, H.S.,Jeong, M.,Del Cacho, E.,Min, W.,Sullivan, Y.B.,Kakach, L.,LaBresh, J.W.,Kim, H.R. Elsevier 2014 Veterinary immunology and immunopathology Vol. No.
Tumor necrosis factor-like ligand 1A (TL1A) is a type II transmembrane protein predominantly expressed by endothelial cells that promotes the expansion of activated T cells and regulatory T cells, modulates inflammation, and regulates the production of a wide variety of T cell cytokines. However, there have not been any mAbs which specifically detect chTL1A and define its biochemical and immunological properties. So in this study, two mouse monoclonal antibodies (mAbs) which specifically detect chicken TL1A (chTL1A) were developed and characterized. Both mAbs identified a 32kDa Escherichia coli-derived, poly-histidine-tagged fusion protein by Western blot analysis. The mAbs identified TL1A-secreting cells in the chicken thymus, cecal tonsil, and bursa of Fabricius by immunocytochemistry, and were used to measure serum TL1A levels in normal and necrotic enteritis (NE)-afflicted chickens by antigen capture ELISA. These mAbs inhibited chTL1A-induced spleen lymphocyte proliferation, nitric oxide production by chicken macrophage cells (HD11), and blocked the cytotoxic effect of chTL1A against lymphoblastoid chicken B tumor cells (LSCC-RP9). These new mAbs that detect chTL1A will be important immune reagents for basic and applied research in poultry immunology.