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Chandra, Dulal,Matsui, Toshiyuki,Suzuki, Haruo,Kosugi, Yusuke,Fujimura, Koichi 한국작물학회 2009 Journal of crop science and biotechnology Vol.12 No.2
The changes in ammonia content as well as activity and gene expression of glutamate dehydrogenase (GDH; EC 1.4.1.2) were investigated in lettuce during storage. GDH amination activity increased with the increases in ammonia content in the outer leaf portion after 24 h of storage. GDH amination activity was substantially higher than deamination activity. The isolated partial cDNA clone referred to as LsGDH (Lactuca sativa glutamate dehydrogenase; AB334207) consisted of 757 nucleotides and was highly homologous with the GDH genes of other plants. Although the transcript of LsGDH was found in both the outer and inner leaves, the level of transcript gradually increased in the outer leaves with the progress of storage, but was only expressed in the inner leaves when higher enzyme activity was observed. Results suggest that GDH expression in lettuce is controlled by tissue specific manner and/or multiple levels of regulations.
Dulal Chandra,Toshiyuki Matsui,Haruo Suzuki,Yusuke Kosugi,Koichi Fujimura 한국작물학회 2009 Journal of crop science and biotechnology Vol.12 No.2
The changes in ammonia content as well as activity and gene expression of glutamate dehydrogenase (GDH; EC 1.4.1.2) were investigated in lettuce during storage. GDH amination activity increased with the increases in ammonia content in the outer leaf portion after 24 h of storage. GDH amination activity was substantially higher than deamination activity. The isolated partial cDNA clone referred to as LsGDH (Lactuca sativa glutamate dehydrogenase; AB334207) consisted of 757 nucleotides and was highly homologous with the GDH genes of other plants. Although the transcript of LsGDH was found in both the outer and inner leaves, the level of transcript gradually increased in the outer leaves with the progress of storage, but was only expressed in the inner leaves when higher enzyme activity was observed. Results suggest that GDH expression in lettuce is controlled by tissue specific manner and/or multiple levels of regulations. The changes in ammonia content as well as activity and gene expression of glutamate dehydrogenase (GDH; EC 1.4.1.2) were investigated in lettuce during storage. GDH amination activity increased with the increases in ammonia content in the outer leaf portion after 24 h of storage. GDH amination activity was substantially higher than deamination activity. The isolated partial cDNA clone referred to as LsGDH (Lactuca sativa glutamate dehydrogenase; AB334207) consisted of 757 nucleotides and was highly homologous with the GDH genes of other plants. Although the transcript of LsGDH was found in both the outer and inner leaves, the level of transcript gradually increased in the outer leaves with the progress of storage, but was only expressed in the inner leaves when higher enzyme activity was observed. Results suggest that GDH expression in lettuce is controlled by tissue specific manner and/or multiple levels of regulations.
살균소독제가 다채 어린잎채소(Brassica campestris var. narinosa)의 수확 후 품질에 미치는 영향
( Dulal Chandra ),김지강 ( Ji Gang Kim ) 한국식품저장유통학회(구 한국농산물저장유통학회) 2011 한국식품저장유통학회지 Vol.18 No.4
The demand of packaged baby leaves has been increased for its convenient use as fresh-cut produce. This investigation was aimed to explore the effects of different sanitizers on the quality parameters of ``Tah Tasai`` Chinese cabbage (Brassica campestris var. narinosa) baby leaves. Thirteen days old baby leaves were harvested and washed in tap water (TW), 100 ppm chlorine solution (Cl), 2 ppm ozonated water (O₃), 15 ppm chlorine dioxide solution (ClO₂) and washing with 0.2% citric acid solution followed by 50% ethanol spray (CA+Et). The samples were then packaged in 50 ?m polyethylene bags and stored at 5℃ for 10 days. Off-odor of packaged baby leaves was not detected during storage. There was no significant difference in color parameters among the treatments. Samples treated with O₂ showed substantially higher electrolyte leakage throughout the storage. This treatment also rendered a higher accumulation of CO₂ in the packages. Samples treated with Cl and CA+Et maintained good overall visual quality with higher scores compared to that of O₃ and ClO₂. Although Cl treatment showed lower number of total aerobic count at the beginning of storage, citric acid in combination with ethanol treatment was more effective until the end of storage. The combined treatment also showed comparatively lower coliform plate count. This result indicates that citric acid wash followed by ethanol spray could be an alternative to chlorine for environment friendly sanitization of baby leaves.
Dulal Chandra,김지강,김용필 한국원예학회 2012 Horticulture, Environment, and Biotechnology Vol.53 No.1
The effects of different sanitizers on the quality and microbial populations of ‘Tah Tasai’ Chinese cabbage (Brassica campestris var. narinosa) microgreens were evaluated. Samples were washed in tap water (TW), 100 mL・L-1chlorinated water (Cl), 0.25% (w/v) each of citric and ascorbic acid mixed solution (CA + AS) as well as 0.5% (w/v)citric acid solution followed by 50% (v/v) ethanol spray (CA + E) and were packaged either in 35 µm polyethylene (PE) or polypropylene (PP) bag and stored at 5℃ for up to 9 days. The decline of O2 partial pressure of the packaged headspace and hue angle values were similar in both films. However, the CO2 partial pressure of the packaged headspace and the electrical conductivity were substantially higher in PP packaged samples than that in PE. Off-odor scores were also higher in PP film packaged samples compared to that of PE. CA + E and CA + AS treatments showed lower off-odor scale in both films. Although, visual quality scores showed similar trends at the beginning of storage, CA + E and CA + AS treated samples exhibited better quality on day 7. Samples washed in Cl showed lower microbial count numbers on washing day. However, CA + E treatment exhibited either the lowest or equivalent count numbers in both total aerobic counts and coliforms count to that of Cl until the end of storage. Considering environmental safety, our results suggest that CA + E treatment could be a useful replacement for Cl for the washing of microgreens and PE film would provide more benefits than PP for packaging microgreens.