Sequence analysis and expression of the calmodulin gene, MCaM-3, in mulberry (Morus L.)

Rongjun Fang,Dongqing Hu,Yinghua Zhang,Long Li,Weiguo Zhao,Li Liu,Jialin Cheng,Jinliang Qi,Yonghua Yang 한국유전학회 2011 Genes & Genomics Vol.33 No.2

A full-length cDNA sequence encoding CaM from mulberry,which we designated MCaM-3 (GenBank accession No:GQ303247), was cloned based on mulberry expressed sequence tags(ESTs). Sequence analysis showed that MCaM-3is 951 base pairs in length, encoding 149 amino acids with a predicted molecular weight of 16.85 kD and an isoelectric point of 3.95. Online SMART analysis showed that the MCaM-3 protein has four EFh functional domains, which can bind calcium. The expression level of MCaM-3at different developmental stages in mulberry leaves and flowers and in different tissues was investigated. The results showed that MCaM-3 transcripts are most abundantly expressed in mature tissues, such as mature female flowers and climax leaves, and the expression level of the mRNA could be increased significantly under low temperature, drought, and salt stress conditions compared to the normal growth environment. This research will help us understand the resistance mechanism of functional genes in mulberry.

Design of Test Platform for Concentrator based on Virtual Electric Energy Meter

Jianying Fan,Hui Sun,Yang Wang,Dongqing Shi 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.9

For the current test of concentrator relay routing learning function in the laboratory, based on the hardware cost and the deployment environment, unable to realize large-scale electric energy meter group network, and real scene environment cannot be simulated. In order to solve these problems, the design of test platform for concentrator based on virtual electric energy meter is proposed in this paper. The design aims to simulate the communication routing network algorithm of electric energy meter and help the communication instruction to establish an optimal topology network which through using the specified data source, target source and the route address. So the concentrator can get the specific data through this preset network. This paper have also observed and analyzed the hierarchy order topology and compared with the autonomous learning topology of the concentrator relay routing algorithm. Then verify the deviation between communication routing topology and preset value, and eliminate the deviation. At the same time, verify whether the relay routing algorithm can get the optimal communication topology network.

Population genetics reveals new introgression in the nucleus herd of min pigs

Liu Tianxin,Ji Dongqing,Li Xinyuan,Liu Jiadong,Xu Fei,Miao Zhiying,Chang Yang,Tian Ming,Xu Chunzhu 한국유전학회 2024 Genes & Genomics Vol.46 No.4

Objective Min pigs are a unique genetic resource among local pig breeds in China. They have more excellent characteristics in cold and stress resistance, good meat quality, and a high reproductive rate. However, the genetic structure and driving factors remain unclear in the nucleus herd. In this study, the genetic diversity of Min pigs was studied to reveal the formation mechanism of its unique genetic structure. We hope to protect and develop the genetic resources of Min pigs. Methods We analyzed different types of genes to identify the genetic structure and gene introgression pattern of Min pigs. The nuclear DNA dataset includes information on 21 microsatellite loci and 6 Y-chromosome genes, and the mitochondrial D-loop gene is selected to represent maternal lineages. The above genes are all from the nucleus herd of Min pigs. Results The results of genetic structure identification and analysis of potential exogenous gene introgression patterns indicate that the nucleus herd of Min pigs maintains a high level of genetic diversity (polymorphism information content = 0.713, expected heterozygosity = 0.662, observed heterozygosity = 0.612). Compared with other Asian pig breeds, the formation of Min pig breeds is more special. Gene introgression from European pig breeds to Min pigs has occurred, which is characterized by complete introgression of paternal genes and incomplete introgression of maternal genes. Conclusion Gene introgression caused by cross-breeding is not the main factor leading to the formation of the current genetic structure of Min pigs, but this process has increased the level of genetic diversity in the nucleus herd. Compared with the influence of gene introgression, our research suggest that artificial selection and environmental adaptive evolution make Min pigs form unique genetic characteristics. Objective Min pigs are a unique genetic resource among local pig breeds in China. They have more excellent characteristics in cold and stress resistance, good meat quality, and a high reproductive rate. However, the genetic structure and driving factors remain unclear in the nucleus herd. In this study, the genetic diversity of Min pigs was studied to reveal the formation mechanism of its unique genetic structure. We hope to protect and develop the genetic resources of Min pigs. Methods We analyzed different types of genes to identify the genetic structure and gene introgression pattern of Min pigs. The nuclear DNA dataset includes information on 21 microsatellite loci and 6 Y-chromosome genes, and the mitochondrial D-loop gene is selected to represent maternal lineages. The above genes are all from the nucleus herd of Min pigs. Results The results of genetic structure identification and analysis of potential exogenous gene introgression patterns indicate that the nucleus herd of Min pigs maintains a high level of genetic diversity (polymorphism information content = 0.713, expected heterozygosity = 0.662, observed heterozygosity = 0.612). Compared with other Asian pig breeds, the formation of Min pig breeds is more special. Gene introgression from European pig breeds to Min pigs has occurred, which is characterized by complete introgression of paternal genes and incomplete introgression of maternal genes. Conclusion Gene introgression caused by cross-breeding is not the main factor leading to the formation of the current genetic structure of Min pigs, but this process has increased the level of genetic diversity in the nucleus herd. Compared with the influence of gene introgression, our research suggest that artificial selection and environmental adaptive evolution make Min pigs form unique genetic characteristics.

In situ observation of mesophase transformation behaviour and mechanistic analysis in β-resin

Liu Ben,Yan Xi,Tao Zechao,Li Xiangfen,Lei Shiwen,Zhang Dongqing,Yang Zonghe,Liu Zhanjun 한국탄소학회 2024 Carbon Letters Vol.34 No.1

β-Resin was extracted by solvent separation of refined coal tar pitch. Several analytical methods revealed that β-resin had a better aromatic plane packing structure and a higher number of carbon residues, making it ideal for mesophase transformation. The mesophase transformation process of β-resin (the formation of liquid-crystalline spheres, the growth of mesophase spheres, and the coalescence and disintegration of mesophase spheres) was observed in situ using a polarizing microscope with a hot stage. Moreover, the mesophase transformation mechanism of β-resin was investigated at each transformation stage. The mesophase content and mesophase transformation kinetics were analyzed based on the area method and quinoline insoluble (QI) substitution method. Both methods revealed changes in the mesophase content of β-resin. However, the test results of the two methods were slightly different at the initial stage of mesophase transformation and tended to be consistent during the later stage.

Thermal-Assisted Single Point Incremental Forming of Jute Fabric Reinforced Poly(lactic acid) Biocomposites

Chang Hou,Xiaobin Su,Xiongqi Peng,Xianfeng Wu,Dongqing Yang 한국섬유공학회 2020 Fibers and polymers Vol.21 No.10

This paper developed a thermal-assisted single point incremental forming (SPIF) method for biodegradablecomposite sheets made from jute woven fabric and polylactic acid (PLA) resin. A forming device equipped with a thermalradiation plate was designed for the SPIF of the composite sheets on a three-axis CNC milling machine. A three-tier-platemethod in which the composite sheet was sandwiched between an iron plate and an alumina plate was adopted to clamp thecomposite sheet. The proposed SPIF method was demonstrated on forming of a truncated pyramid part. The effects ofclamping method, toolpath, step size and thickness on the formability of the composite were investigated. It was found thatthe three-tier-plate fixture method was crucial for the successful implementation of the SPIF of the composite. The toolpathhad a significant effect on the forming depth. While the surface quality was mainly determined by the step size. The thicknessof the composite sheet had a positive effect on is forming depth.