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        Amperometric sensing of sodium, calcium and potassium in biological fluids using a microhole supported liquid/gel interface

        Diba, F.S.,Lee, H.J. Elsevier Sequoia 2016 Journal of Electroanalytical Chemistry Vol.769 No.-

        <P>In this paper, we successfully applied a cost-effective and simple amperometric sensing design featuring a single microhole-water/organic gel interface to determine native sodium, calcium and potassium ion concentrations in biological fluids. The detection was based on measuring current changes associated with the transfer of each cation type across a microhole supported water/polyvinylchloride-2-nitrophenyl octylether gel interface. Three different sensing platforms were applied to the same biological sample with each only differing in the selective ionophore incorporated into the organic phase: dibenzo-18-crown-6 for sodium, calcium ionophore II (ETH 129) for calcium and valinomycin for potassium ions. Assisted transfer reactions of each ion from the aqueous to the organic gel layer by the corresponding ionophores were first characterized using cyclic voltammetry and then further analyzed using differential pulse stripping voltammetry. A linear dynamic range from 5 mu M to 100 mu M was achieved for each ion target. This enabled quantitative measurements of native concentrations of Na+, Ca2+ and K+ in biological fluid samples including serum and plasma. The amperometric measurements were also validated by comparing with inductively coupled plasma mass spectrometry. (C) 2016 Elsevier B.V. All rights reserved.</P>

      • Electrochemical immunoassay for amyloid-beta 1–42 peptide in biological fluids interfacing with a gold nanoparticle modified carbon surface

        Diba, Farhana Sharmin,Kim, Suhee,Lee, Hye Jin Elsevier 2017 CATALYSIS TODAY - Vol.295 No.-

        <P><B>Abstract</B></P> <P>An electrochemical immunosensor involving the formation of a surface sandwich complex on a gold nanoparticle (NP) modified screen printed carbon electrode (SPCE) is demonstrated for the femtomolar detection of amyloid-beta 1–42 peptide (Aβ) in both serum and plasma. Both bioreceptors forming the assay are highly selective antibodies for Aβ, namely antiAβ (12F4) and (1E11) which possess different binding sites for the Aβ peptide. In order to improve the sensing performance for complex biological fluidic matrix analysis, different mixed monolayers of thiol modified polyethylene glycol (PEG) and mercaptopropionic acid (MPA) were self-assembled onto the Au NP-SPCE followed by tethering antiAβ (12F4) to MPA using a heterobifunctional cross linker. Surface sandwich complexes of antiAβ (12F4)/Aβ/antiAβ (1E11)-ALP were then formed via sequential adsorption with the latter antiAβ (1E11) conjugated to alkaline phosphatase (ALP) enzyme. The reaction of surface immobilized ALP with the substrate, 4-amino phenyl phosphate (APP), generated voltammetric detection signals that linearly increased as a function of Aβ concentration. Differential pulse voltammetry was applied to establish a lowest detectable concentration of 100 fM of Aβ with a linear response range from 100 fM to 25 pM. Following optimization, the immunoassay platform was applied in diluted human serum and plasma samples to determine the native concentration of Aβ and the results were validated using a commercially available ELISA test.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A highly sensitive and sensitive sandwich assay for amyloid-beta 1–42 peptide (Aβ). </LI> <LI> Formation of antiAβ (12F4)/Aβ/antiAβ (1E11)-ALP surface sandwich complex. </LI> <LI> Use of a mixed monolayer for sensors surface to improve non-specific adsorption. </LI> <LI> Direct analysis of Aβ in diluted human serum and plasma samples. </LI> <LI> A lowest detectable concentration of 100 fM Aβ with dynamic range of 0.1–25 pM. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>A new electrochemical-based surface sandwich assay constructed on Au nanoparticles deposited on a screen printed carbon electrode was developed for the femtomolar detection of unmodified amyloid-beta 1–42 (Aβ) in serum and plasma samples at native concentrations.</P> <P>[DISPLAY OMISSION]</P>

      • A Study of Rainfall Impacts on Terahertz Wireless Communication Systems

        Feyisa Debo Diba,Sungoh Kwon 한국통신학회 2022 한국통신학회 학술대회논문집 Vol.2022 No.2

        In this paper, rain rate data measured at one-minute interval is used to determine the cumulative probability distribution, giving the rainfall rate versus the percentage of time the indicated rainfall was exceeded in a year. Then based on this result, the specific rain attenuation is calculated for frequencies up to 1000 GHz using ITU-R model. The result shows, rain will not produce any additional attenuation for operating frequencies above 100 GHz.

      • KCI등재

        The Antitermitic and Antifungal Activities and Composition of Vinegar from Durian Wood (Durio sp.)

        Awan SUPRIANTO,Hasan Ashari ORAMAHI,Farah DIBA,Gusti HARDIANSYAH,M. Sofwan ANWARI 한국목재공학회 2023 목재공학 Vol.51 No.4

        Chemical characterization of vinegars obtained from Durian wood (Durio sp.) and their termicidal activity against Coptotermes curvignathus and antifungal activity against Schizophyllum commune were evaluated. The process of pyrolysis produced wood vinegars at three distinct temperature: 350℃, 400℃, and 450℃. To determine their effectiveness against fungal growth, the vinegars were tested using a Petri dish with 1.0%, 2.0%, 3.0%, and 4.0% (v/v) against S. commune. In the experiment, termicidal activities were evaluated using a no-choice test for C. curvignathus with 3.0%, 6.0%, 9.0%, and 12.0% (v/v). The wood vinegar exhibited antitermitic activity to C. curvignathus workers in the no-choice experiment; For vinegar produced at 450℃, a 6% concentration was required to achieve 100% mortality against C. curvignathus. In addition, a 12% vinegar produced at 450℃ resulted in the lowest mass loss of treated filter paper, which was 20.00%. Furthermore, all the wood vinegars exhibited antifungal activities against S. commune at concentration of 2.0%. The dominant chemical components of wood vinegar produced at temperature of 350℃, 400℃, and 450℃ were 2-methoxy-phenol, 4-ethyl-2-methoxy-phenol, 4-ethyl-2-methoxy-phenol, 3.5-dimethoxy-4-hydroxytoluene, and creosol.

      • SCOPUSKCI등재

        Termicidal Activity and Chemical Components of Wood Vinegar from Nipah Fruit against Coptotermes curvignathus

        ORAMAHI, Hasan Ashari,TINDAON, Mika Jessika,NURHAIDA, Nurhaida,DIBA, Farah,YANT, Hikma The Korean Society of Wood ScienceTechnology 2022 목재공학 Vol.50 No.5

        The termicidal activity and chemical components of wood vinegar from two sources of biomass, nipah fruit shells (NFS) and a mixture of shells and fiber (MSF), were evaluated against Coptotermes curvignathus. A no-choice test was carried out to evaluate their termicidal activity using filter paper samples treated with 2.5%, 5.0%, 7.5%, 10.0%, and 12.5% NFS or MSF vinegar. Both wood vinegars exhibited antitermitic activity against C. curvignathus. The results show that increased concentrations of NFS and MSF vinegar significantly increased termite mortality. In particular, the NFS vinegar caused complete mortality and the lowest filter paper mass losses at 2.18% when treated with 12.5% wood vinegar. The most abundant chemical compounds of NFS vinegar were cyclopropanecarbonyl chloride, 2,5-dichlorophenol, 2-propanone, acetic acid, propanoic acid, benzenesulfonic acid, 3,7-dimethyl-6-octenal, and trans-geraniol. Meanwhile, the main compounds in the MSF vinegar were 1,2-ethanediol, formic acid, acetic acid, ethanoic acid, 2-furancarboxaldehyde, phenol, 2-methoxy phenol, and 4-methyl phenol.

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