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Xiaoqian Wang,Chunyan Liu,Qiang Wang,Chang Liu,Huili Sun,Maojun Zhang,Deguo Lyu,Guodong Du 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.3
Pyrus ussuriensis is the most important cultivated pear in the northeastern, cold areas of China. However, studies on thebiological activity of Pyrus ussuriensis fruit are rare in the public domain. The present study compared antioxidant, antiinflammatory, and antimicrobial activities and chemical composition in tissues from the peel and fl esh of nine Pyrus ussuriensiscultivars. The chemical composition diff ered among cultivars and tissue sources. Phenolics were predominant in peelsamples, whereas sugars were predominant in fl esh samples. Twenty-one phenolic compounds were identifi ed and quantifi ed,including hydroxycinnamic acids, hydroquinones, fl avanols, fl avonols, fl avones, and anthocyanins (only in peel samples). The total phenolic content ranged between 363.0 and 1734.0 mg kg − 1 FW in the peel and from 28.5 to 182.3 mg kg − 1 FWin the fl esh. In addition, cultivars with high total phenolic and fl avonoid contents had signifi cantly higher antioxidant activities. The in vitro anti-infl ammatory study, performed using an egg albumin denaturation assay, demonstrated that both peeland fl esh samples had strong activity, which was comparable with that of the standard anti-infl ammatory drug diclofenacsodium. The antimicrobial results showed that ‘Jianba’ and ‘Balixiang’ cultivars exhibited strong activity against bacteriastrains in the peel and fl esh, respectively. The present study provided information for selecting promising Pyrus ussuriensiscultivars with improved health benefi ts.
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Yongzhen Wang,Borui Wang,Dandan Xu,Meng Zhang,Xiaohua Zhang,Deguo Wang 대한수의학회 2022 Journal of Veterinary Science Vol.23 No.4
Background: Due to the unavailability of an effective vaccine or antiviral drug against the African swine fever virus (ASFV), rapid diagnosis methods are needed to prevent highly contagious African swine fever. Objectives: The objective of this study was to establish the ladder-shape melting temperature isothermal amplification (LMTIA) assay for the detection of ASFV. Methods: LMTIA primers were designed with the p72 gene of ASFV as the target, and plasmid pUC57 was used to clone the gene. The LMTIA reaction system was optimized with the plasmid as the positive control, and the performance of the LMTIA assay was compared with that of the commercial real-time polymerase chain reaction (PCR) kit in terms of sensitivity and detection rate using 200 serum samples. Results: Our results showed that the LMTIA assay could detect the 104 dilution of DNA extracted from the positive reference serum sample, which was the same as that of the commercial real-time PCR kit. The coincidence rate between the two assays was 100%. Conclusions: The LMTIA assay had high sensitivity, good detection, and simple operation. Thus, it is suitable for facilitating preliminary and cost-effective surveillance for the prevention and control of ASFV.
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