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RISS 인기검색어
- 검색키워드
- 저자 : Debashis Roy (검색결과 3 건)
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Roy Debashis,Chakraborty Gautam,Biswas Abhisek,Sarkar Pijush Kanti 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.1
Twenty-six rice landraces from West Bengal, India were evaluated for antixenosis and tolerance against brown planthopper (BPH) biotype 4 at the Bidhan Chandra Krishi Viswavidyalaya (BCKV), West Bengal. High levels of resistance were observed in six landraces, namely Badshabhog, Gamra, Haldichuri, Janglijata, Kalabhat and Khara. These phenotypically resistant rice landraces including Ptb33 exhibited lowest feeding rate, fecundity, nymphal and adult preference, survival, plant dry weight loss per mg of BPH dry weight produced (PDWL), and higher functional plant loss index (FPLI), more days to wilt and unhatched eggs compared with the susceptible check Swarna. All the landraces were classified into four major clusters at 10 unit distance by the scale of similarity during genetic diversity analysis through 21 gene-linked SSR markers of BPH resistance. Some phenotypically resistant landraces were gathered under the major cluster I indicating their analogous genetic history, while some were grouped with susceptible landraces exhibiting their genetic variation. The resistant landraces can be used as potential donors in the breeding programme for the development of rice varieties with resistance to BPH.
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Can insecticide mixtures be considered to surmount neonicotinoid resistance in Bemisia tabaci?
Roy Debashis,Biswas Sujan,Biswas Abhisek,Chakraborty Gautam,Sarkar Pijush Kanti 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.2
Cotton whitefly, Bemisia tabaci is an important polyphagous pest worldwide. It is exposed to various chemical insecticides throughout the year, resulting in the rapid development of insecticide resistance. Mixtures of in secticides with distinct modes of action could enhance the toxicity of chemicals more effectively than sequences or rotations in resistant pest populations. Bioassays were conducted to study the efficacy of mixtures of neon icotinoid and ketoenol insecticides at different ratios against a laboratory susceptible (Lab-WB) and a neon icotinoid resistant (TMX-SEL) strain of B. tabaci Asia I. The results showed that mixtures of imidacloprid, acetamiprid, thiamethoxam or dinotefuran with spiromesifen at 1:1, 1:10 and 1:20 ratios and of imidacloprid, thiamethoxam or dinotefuran with spirotetramat at 1:1 ratio significantly increased (p < 0.05) toxicity to neonicotinoids in TMX-SEL strain. The combination indices of each tested neonicotinoids + ketoenols at 1:1 ratio and of acetamiprid + spiromesifen, and imidacloprid or dinotefuran + spirotetramat at 1:10 ratio for TMX-SEL strain were significantly below 1, suggesting synergistic interactions. The inhibitors PBO and DEF largely overcame resistance to the tested neonicotinoids, while none of the synergists significantly restored the sus ceptibility of B. tabaci to ketoenols. Increased activities of P450 monooxygenase and esterase were observed in TMX-SEL strain with an elevated 2.76 and 1.32-fold, respectively. Mixtures of neonicotinoids with spiromesifen or spirotetramat at a 1:1 ratio could be used to restore the neonicotinoid susceptibility in B. tabaci.
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Antibiotic Production by a Marine Isolate (MS 310) in An Ultra-low-speed Rotating Disk Bioreactor
Sreyashi Sarkar,Joydeep Mukherjee,Debashis Roy 한국생물공학회 2009 Biotechnology and Bioprocess Engineering Vol.14 No.6
Actinomycin-D production by a biofilm-forming estuarine isolateviz. Streptomyces sp. MS 310 is studied in smallscale shaken cultures, as well as in a 25 L rotating disk bioreactor, (RDBR) which, when operated at a disk rotational speed of 1 revolution/day with 50% disk submergence, mimics the intertidal conditions of the microbe’s niche estuarine habitat- alternating 12 h periods of inundation and exposure. The ideal pH and temperature for antibiotic production are determined (pH 10, 30oC) through a designed experimental study using shaken flasks. Subsequently, operating conditions in the RDBR are investigated employing a 3ⁿ experimental design wherein each of two (n = 2) parameters viz. aeration and disk submergence are considered at three levels viz. high, medium, and low: 9.0, 6.0, and 3.0 L/min for aeration rate; and 75, 50, and 25% for disk submergence, (while maintaining the rotational speed at 1.0 rev/day). The niche-mimic condition along with maximum permissible aeration is found to be most favorable for antibiotic production - peak antibiotic activity (PAA) and peak activity attainment rate (PAAR) simultaneously attaining their highest values: 40 mm and 2.13 mm/h, respectively. Both PAA and PAAR increase with increasing aeration at all operating conditions examined - particularly, at the niche-mimic condition, a threefold increase in aeration rate (3~9 L/min) causes PAA to increase by 33%, whereas PAAR increases by 2.5 times, thus pointing to the strong aeration dependence of this actinomycin-D producer. Again, compared to the best values obtained in the 500 mL shaken flask experiments, corresponding RDBR values are 16% higher for PAA and more than 5 times higher for PAAR - strong evidence for employing these novel bioreactors for cultivation of antibiotic-producer marine microbes Actinomycin-D production by a biofilm-forming estuarine isolateviz. Streptomyces sp. MS 310 is studied in smallscale shaken cultures, as well as in a 25 L rotating disk bioreactor, (RDBR) which, when operated at a disk rotational speed of 1 revolution/day with 50% disk submergence, mimics the intertidal conditions of the microbe’s niche estuarine habitat- alternating 12 h periods of inundation and exposure. The ideal pH and temperature for antibiotic production are determined (pH 10, 30oC) through a designed experimental study using shaken flasks. Subsequently, operating conditions in the RDBR are investigated employing a 3ⁿ experimental design wherein each of two (n = 2) parameters viz. aeration and disk submergence are considered at three levels viz. high, medium, and low: 9.0, 6.0, and 3.0 L/min for aeration rate; and 75, 50, and 25% for disk submergence, (while maintaining the rotational speed at 1.0 rev/day). The niche-mimic condition along with maximum permissible aeration is found to be most favorable for antibiotic production - peak antibiotic activity (PAA) and peak activity attainment rate (PAAR) simultaneously attaining their highest values: 40 mm and 2.13 mm/h, respectively. Both PAA and PAAR increase with increasing aeration at all operating conditions examined - particularly, at the niche-mimic condition, a threefold increase in aeration rate (3~9 L/min) causes PAA to increase by 33%, whereas PAAR increases by 2.5 times, thus pointing to the strong aeration dependence of this actinomycin-D producer. Again, compared to the best values obtained in the 500 mL shaken flask experiments, corresponding RDBR values are 16% higher for PAA and more than 5 times higher for PAAR - strong evidence for employing these novel bioreactors for cultivation of antibiotic-producer marine microbes
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