http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Sreyashi Sarkar (검색결과 1 건)
- 무료
- 기관 내 무료
- 유료
-
Antibiotic Production by a Marine Isolate (MS 310) in An Ultra-low-speed Rotating Disk Bioreactor
Sreyashi Sarkar,Joydeep Mukherjee,Debashis Roy 한국생물공학회 2009 Biotechnology and Bioprocess Engineering Vol.14 No.6
Actinomycin-D production by a biofilm-forming estuarine isolateviz. Streptomyces sp. MS 310 is studied in smallscale shaken cultures, as well as in a 25 L rotating disk bioreactor, (RDBR) which, when operated at a disk rotational speed of 1 revolution/day with 50% disk submergence, mimics the intertidal conditions of the microbe’s niche estuarine habitat- alternating 12 h periods of inundation and exposure. The ideal pH and temperature for antibiotic production are determined (pH 10, 30oC) through a designed experimental study using shaken flasks. Subsequently, operating conditions in the RDBR are investigated employing a 3ⁿ experimental design wherein each of two (n = 2) parameters viz. aeration and disk submergence are considered at three levels viz. high, medium, and low: 9.0, 6.0, and 3.0 L/min for aeration rate; and 75, 50, and 25% for disk submergence, (while maintaining the rotational speed at 1.0 rev/day). The niche-mimic condition along with maximum permissible aeration is found to be most favorable for antibiotic production - peak antibiotic activity (PAA) and peak activity attainment rate (PAAR) simultaneously attaining their highest values: 40 mm and 2.13 mm/h, respectively. Both PAA and PAAR increase with increasing aeration at all operating conditions examined - particularly, at the niche-mimic condition, a threefold increase in aeration rate (3~9 L/min) causes PAA to increase by 33%, whereas PAAR increases by 2.5 times, thus pointing to the strong aeration dependence of this actinomycin-D producer. Again, compared to the best values obtained in the 500 mL shaken flask experiments, corresponding RDBR values are 16% higher for PAA and more than 5 times higher for PAAR - strong evidence for employing these novel bioreactors for cultivation of antibiotic-producer marine microbes Actinomycin-D production by a biofilm-forming estuarine isolateviz. Streptomyces sp. MS 310 is studied in smallscale shaken cultures, as well as in a 25 L rotating disk bioreactor, (RDBR) which, when operated at a disk rotational speed of 1 revolution/day with 50% disk submergence, mimics the intertidal conditions of the microbe’s niche estuarine habitat- alternating 12 h periods of inundation and exposure. The ideal pH and temperature for antibiotic production are determined (pH 10, 30oC) through a designed experimental study using shaken flasks. Subsequently, operating conditions in the RDBR are investigated employing a 3ⁿ experimental design wherein each of two (n = 2) parameters viz. aeration and disk submergence are considered at three levels viz. high, medium, and low: 9.0, 6.0, and 3.0 L/min for aeration rate; and 75, 50, and 25% for disk submergence, (while maintaining the rotational speed at 1.0 rev/day). The niche-mimic condition along with maximum permissible aeration is found to be most favorable for antibiotic production - peak antibiotic activity (PAA) and peak activity attainment rate (PAAR) simultaneously attaining their highest values: 40 mm and 2.13 mm/h, respectively. Both PAA and PAAR increase with increasing aeration at all operating conditions examined - particularly, at the niche-mimic condition, a threefold increase in aeration rate (3~9 L/min) causes PAA to increase by 33%, whereas PAAR increases by 2.5 times, thus pointing to the strong aeration dependence of this actinomycin-D producer. Again, compared to the best values obtained in the 500 mL shaken flask experiments, corresponding RDBR values are 16% higher for PAA and more than 5 times higher for PAAR - strong evidence for employing these novel bioreactors for cultivation of antibiotic-producer marine microbes
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
