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RISS 인기검색어
- 검색키워드
- 저자 : David J. Beebe (검색결과 4 건)
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이정훈,김영웅,David J. Beebe,김병규 한국바이오칩학회 2012 BioChip Journal Vol.6 No.3
We present a negative dielectrophoretic (n-DEP) force based high throughput cell sorting system integrating a cantilever-type electrode array in a vertical macro channel to leverage gravity driven flow and eliminate the need for external pumps. The system comprises a macro-sized channel to increase throughput, a cantilever electrode array(L×W×H=150μm×500μm×10μm) to achieve n-DEP force and high throughput, and a flow regulator to precisely control hydrodynamic force. The hydrodynamic force and the n-DEP force acting on the target cell are evaluated theoretically. In addition, optimal separation conditions are investigated using computational models. Separation conditions are experimentally investigated based on simulation results. Finally, to demonstrate the separation performance of the sorting system, we performed the separation of the human breast cancer cells (MCF 7) from diluted red blood cells(RBCs) under conditions of low voltage(7Vp-p with 500kHz) and flow rate of 5μL∙min-1 . The system can separate MCF 7 cell with 71% separation efficiency in case of the ratio of 1:60000(MCF 7:RBCs).
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A Cell Programmable Assay (CPA) chip
Ju, Jongil,Warrick, Jay,Beebe, David J. Royal Society of Chemistry 2010 Lab on a chip Vol.10 No.16
<P>This article describes two kinds of “Cell Programmable Assay” (CPA) chips that utilize passive pumping for the culture and autonomous staining of cells to simply common protocols. One is a single timer channel CPA (sCPA) chip that has one timer channel and one main channel containing a cell culture chamber. The sCPA is used to culture and stain cells using Hoechst nuclear staining dye (a 2 step staining process). The other is a dual timer channel CPA (dCPA) chip that has two timer channels and one main channel with a chamber for cell culture. The dCPA is used here to culture, fix, permeablize, and stain cells using DAPI. The additional timer channel of the dCPA chip allows for automation of 3 steps. The CPA chips were successfully evaluated using HEK 293 cells. In addition, we provide a simplified equation for tuning or redesigning CPA chips to meet the needs of a variety of protocols that may require different timings. The equation is easy to use as it only depends upon the dimensions of microchannel and the volume of the reagent drops. The sCPA and dCPA chips can be readily modified to apply to a wide variety of common cell culture methods and procedures.</P> <P>Graphic Abstract</P><P>We present “Cell Programmable Assay” (CPA) chips that utilize passive pumping for the culture and autonomous staining of cells to simply common protocols. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c005103a'> </P>
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Biomimetic Soft Multifunctional Miniature Aquabots
Kwon, Gu Han,Park, Joong Yull,Kim, Jeong Yoon,Frisk, Megan L.,Beebe, David J.,Lee, Sang-Hoon WILEY-VCH Verlag 2008 Small Vol.4 No.12
<B>Graphic Abstract</B> <P>Small (micro- to millimeter) soft polymeric aquabots that perform multifunctional operations in aqueous environments, effectively simulating their natural counterparts (see image)are presented. These aquabots have diverse muscle-like locomotive mechanisms as well as integrated organs including body structures, sensors, and drug-releasing systems. The demonstrated functions have potential applications in drug delivery, environmental monitoring, and inspection. <img src='wiley_img/16136810-2008-4-12-SMLL200800315-content.gif' alt='wiley_img/16136810-2008-4-12-SMLL200800315-content'> </P>
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