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Cho, Eun Sook,Jin, Byung Rae,Sohn, Hung Dae,Choi, Kwang Ho,Kim, Soung Ryul,Kang, Seok Woo,Yun, Eun Young,Kim, Sang Hyun,Kim, Keun Young,Je, Yeon Ho,Kang, Seok Kwon 한국잠사학회 1998 한국잠사곤충학회지 Vol.40 No.2
To construct transfurmed Bm5 cells, Autographa californica nuclear polyhedrosis virus(AcNPV) IE1 gene, an immediate early viral gene was firstly used in this study. AcNPV IE1 gene, which shares on 95.3% nucleotide sequence homology with Bombyx mori nuclear polyhedrosis virus (BmNPV) IE1 gene, was isolated and cloned into pBluescript. Neomycin gene from pKO-neo was inserted under the control of the IE1 promoter to yield pAcIE1-neo. The plasmid pAcIE1-neo was transfected into Bm5 or Sf9 cells, and neomycin-resistant cells were selected in TC100 medium containing 10% fetal bovine serum (FBS) and 1 mg/ml G4l8 for two weeks. Individual clones were picked and each was amplified for further characterization. The genomic DNA from neomycin-resistant cells was isolated and characterized by PCR using AcNPV IEI gene-specific primers and by Southern blot analysis using neomycin gene probe. We concluded that AcNPV IE1 gene was functional in B. mori-derived Bm5 cells as well as Spodoptera fugjprrda-derived Sf9 cells to produce stably-transformed insect cells
Sung-Dae Cho,Nam-Pyo Cho 대한구강악안면병리학회 2007 대한구강악안면병리학회지 Vol.31 No.5
Nerve gro때h factor-induced B (NGFI-B, Nur77) is an orphan nuclear receptor with no known endogenous Iigands , however‘ recent stuclies on a series of methylene -substituted diindolylmethanes (C-DIMs) have identified 1,l-bis(3’ - In dolyl) -l-(phenyl)methane (DIM-C-Ph) and l , l -bis(3’ indolyl)-l-(p-anisyl)methane (DIM-C-pPhOCHa) as Nur77 agonist Nur77 is expressed in several colon cancer cell lines (RKO, SW480, HCT-116, HT-29 and HCT-15) and we a lso observed by irnmunostaining that Nur77 was overexpressed in colon tumors compared to normal colon tIssue DIM-C-Ph and DlM-C-pPhOCH3 decreased survival and induced apoptosis in RKO colon cancer cells and this was accompanied by in ductdion of tumor nec rosis factor-related apoptosis-incluced ligand (TRAlL) protein, The induct ion of a poptosis and TRAlL by DIM-C-pPhOCH3 was significantly inhibited by a small inhibitory RNA for Nur77 (iNur77); however, it was evide nt from RNA in terference studies that DIM-C-pPhOCH3 a1so induced Nur77-independent apoptosis. Analysis o( DIM-C-pPhOCH3-induced gene expression using microarrays idontifiod sovoral proapoptotic genos and analysis by ro verse t ranscriptase PCR in the presence 0 1' absence of iNru77 showed that incluction of prograrnmed cell death gene 1 (PDcm) was Nur77-dependent‘ whereas induction of cystathionase (CSE) and activating transcription factor 3 (ATF3) was Nur77-independent, DIM-C-pPhOCHa (25 mg/kg/day) also inhi bited tumor growth in athymic nude mice bearing RKO cell xenograft, These results demonstrate that Nur77-active C-DIM compounds represent a new class of anti-colon cancer drugs that act through receptor- dependent and - independent pathway
( Dae Won Jun ),( Yong Kyun Cho ),( Seung Min Lee ),( Eun Chul Jang ),( Se Whan Lee ),( Ki Tae Suk ),( Sang Bong Ahn ) 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1
Background: Until now there is no specialized diet education program in nonalcoholic fatty liver disease (NAFLD). So, diet education program for obesity or dyslipidemia have been used to NAFLD patients in Korea. Both conventional diet programs mainly stressed on reducing fat consumption. However fat energy percent is less than 20% in Korea. We would like to investigate the efficacy and compliance of low carbohydrate diet in Korean NAFLD patients. Methods: One hundred and six NAFLD patients were enrolled from five hospitals. The patients were randomly selected to the conventional obesity diet program and low carbohydrate program. Liver chemistry, liver/spleen ratio, visceral fat CT scan, and serum CK-18 were measured at baseline and after 8 weeks. All participants completed five-day diet diary survey twice before and after diet education. Diagnosis of NAFLD was based on sonographic fat infiltration with elevated aminotransferase activity. Results: Both conventional diet program and low carbohydrate diet program decreased body weight and waist circumference. However, only low carbohydrate group showed significant decrease in ALT, AST, LDL-cholesterol, and blood pressure level compared to baseline. The ALT normalization at 8 weeks was 38.5% for the low carbohydrate and 16.7% for the low fat group (P=0.016). More than 80% of low carbohydrate group decreased serum ALT activity, while only 57% of conventional low fat group decreased ALT level compare to base line (P=0.012). Total abdominal fat area (401.3±184.3 vs. 378.0 ± 166.3, P=0.0001) and liver/spleen HU ratio (0.88 ± 0.25 vs. 0.92 ± 0.24, P=0.015) were decreased from the baseline in only low carbohydrate group. Not only carbohydrate consumption level but also total energy intake and fat consumption levels decreased more in low carbohydrate group than conventional anti- obesity program. Compliance of both two programs and physical activities during follow up period were not difference. Conclusions: Low carbohydrate diet program is more effective in reducing total energy intake and ALT normalization in NAFLD patients in Korea.
Poster Session 1 : Isoproterenol modulates the expression of RGS2 in C6 rat glioma cells
( Sung Dae Kim ),( Hui Min Lee ),( Hye Jin Sung ),( Sun Kyu Park ),( Tae Wan Kim ),( Kil Soo Kim ),( Jae Chan Song ),( Seung Chun Park ),( Won Gi Min ),( Jae Youl Cho ),( Man Hee Rhee ) 한국생화학분자생물학회 (구 한국생화학회) 2005 생화학분자생물학회 춘계학술발표논문집 Vol.2005 No.-
( Dae Won Jun ),( Byung Ik Kim ),( Yong Kyun Cho ),( Hong Ju Kim ),( Young Oh Kwon ),( Soo Young Park ),( Sang Young Han ),( Yang Hyun Baek ),( Yong Jin Jung ),( Hwi Young Kim ),( Won Kim ),( Jeong He 대한간학회 2013 Clinical and Molecular Hepatology(대한간학회지) Vol.19 No.2
Background/Aims: Carnitine and vitamin complex (Godex®) is widely used in patients with chronic liver disease who show elevated liver enzyme in South Korea. The purpose of this study is to identify the efficacy and safety of carnitine from entecavir combination therapy in Alanine aminotransferase (ALT) elevated Chronic Hepatitis B (CHB) patients. Methods: 130 treatment-na?ve patients with CHB were enrolled from 13 sites. The patients were randomly selected to the entecavir and the complex of entecavir and carnitine. The primary endpoint of the study is ALT normalization level after 12 months. Results: Among the 130 patients, 119 patients completed the study treatment. The ALT normalization at 3 months was 58.9% for the monotherapy and 95.2% for the combination therapy (P<0.0001). ALT normalization rate at 12 months was 85.7% for the monotherapy and 100% for the combination group (P=0.0019). The rate of less than HBV DNA 300 copies/mL at 12 months was not statistically significant (P=0.5318) 75.9% for the monotherapy, 70.7% for the combination and it was. Quantification of HBsAg level was not different from the monotherapy to combination at 12 months. Changes of ELISPOT value to evaluate the INF-Y secretion by HBsAg showed the increasing trend of combination therapy compare to mono-treatment. Conclusions: ALT normalization rate was higher in carnitine complex combination group than entecavir group in CHB. Combination group was faster than entecavir mone-treatment group on ALT normalization rate. HBV DNA normalization rate and the serum HBV-DNA level were not changed by camitine complex treatment.
Cho, Eun-Sook,Jin, Byung-Rae,Sohn, Hung-Dae,Chol, Kwang-Ho,Kim, Soung-Ryul,Kang, Seok-Woo,Yun, Eun-Young,Kim, Sang-Hyun,Kim, Keun-Young Korean Society of Sericultural Science 1998 한국잠사곤충학회지 Vol.40 No.2
To construct transformed Bm5 cells, Autographa californica nuclear polyhedrosis virus (AcNPV)IE1 gene, an immediate early viral gene was firstly used in this study. AcNPV IE1 gene, which shares on 95.3% uncleotide sequence homology with Bombyx mori nuclear polyhedrosis virus (BmNPV) IE1 gene, was isolated and cloned into pBluescript. Neomycin gene from pKO-neo was inserted under the control of the IE1 promoter to yield pAcIE1-neo. The plasmid pAcIE1-neo was transfected into Bm5 or Sf9 cells, and neomycin-resistant cells were selected in TC100 medium containing 10% fetal bovine serum (FBS) and 1 mg/$m\ell$ G418 for two weeks. Individual clones were picked and each was amplified for further characterization. The genomic DNA from neomycin-resistnt cells was isolated and characterized by PCR using AcNPV IE1 gene-specific primers and by Southern blot analysis using neomycin gene probe. We concluded that AcNPV IE1 gene was functional in B. moridrived Bm5 cells as well as Spodaptera frugiperda-derived Sf9 cells to produce stably-transformed insect cells.
( Dae Kyung Bae ),( Jong Whan Lee ),( Seong Jin Cho ),( Sang Jun Song ) 대한슬관절학회 2017 대한슬관절학회지 Vol.29 No.3
Purpose: To compare navigation and weight bearing radiographic measurements of mechanical axis (MA) before and after closed wedge high tibial osteotomy (HTO) and to evaluate post-osteotomy changes in MA assessed during application of external varus or valgus force. Materials and Methods: Data from 30 consecutive patients (30 knees) who underwent computer-assisted closed-wedge HTO were prospectively analyzed. Pre-and postoperative weight bearing radiographic evaluation of MA was performed. Under navigation guidance, pre-and post-osteotomy MA values were measured in an unloaded position. Any change in the post-osteotomy MA in response to external varus or valgus force, which was named as dynamic range, was evaluated with the navigation system. The navigation and weight bearing radiographic measurements were compared. Results: Although there was a positive correlation between navigation and radiographic measurements, the reliability of navigation measurements of coronal alignment was reduced after osteotomy and wedge closing. The mean post-osteotomy MA value measured with the navigation was 3.5°±0.8° valgus in an unloaded position. It was 1.3°±0.8° valgus under varus force and 5.8°±1.1° valgus under valgus force. The average dynamic range was >±2°. Conclusions: Potential differences between the postoperative MAs assessed by weight bearing radiographs and the navigation system in unloaded position should be considered during computer-assisted closed wedge HTO. Care should be taken to keep the dynamic range within the permissible range of alignment goal in HTO.