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RISS 인기검색어
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- 저자 : Chaohui Zhu (검색결과 4 건)
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Synthesis of Cefprozil Using Penicillin G Acylase in Recyclable Aqueous Two-phase Systems
Chaohui Zhu,Xue-Jun Cao 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.5
Cefprozil is an important semi-syntheticcephalosporin antibiotic. In this study, immobilized penicillinG acylase (PGA) is used to catalyze the acylation of 7-amino-3-(1-propenyl)-4-cephalosporanic acid (7-APRA)and 4-hydroxyphenylglycine methyl ester (HPGME) and arecyclable thermo-pH responsive PNB/PADB aqueous twophasesystem (ATPS) is used to synthesize cefprozil. In thissystem, the partition coefficient of cefprozil was 2.24 with60 mmol/L (NH4)2SO4. In addition, the optimal enzymaticreaction conditions were found to be pH 6.5, 20°C, 78 u/mLimmobilized PGA, 30 mmol/L 7-APRA and 90 mmol/LHPGME. In the PNB/PADB ATPS, the maximal yield ofcefprozil was 75.81% with 60 mmol/L (NH4)2SO4 and inthe single aqueous system the yield was 56.02%. Theyields are thought to improve because there is a reductionin product inhibition.
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( Huatuo Zhu ),( Xinyong Wan ),( Wenguo Chen ),( Chaohui Yu ),( Min Yue ),( Yining Dai ),( Lihua Chen ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: It was recently reported that epigenetics might play an essential role in IBD. Bromo adjacent homology domain 1 (BAHD1), which involved in category of epigenetics maintains homeostasis by promoting heterochromatin formation. Our study aimed to investigate the underlying mechanism of BAHD1 in gut inflammation including IBD, seeking a new therapeutic target for the inflammation-associated colon disease. Methods: Experimental colitis was induced in C57BL/6 mice by dextran sulfate sodium administration. To simulate the intestinal inflammation microenvironment for epithelial cells, Caco-2 cells were exposed to a mixture of LPS, TNF-a, IL-1ß, and IFN-. BAHD1 expression was detected by quantitative PCR(qPCR), western blot and immunohistochemistry in both UC patients and mice model. Small interfering RNA was used to knock down BAHD1 level(siBAHD1) in Caco-2 cells and associated cytokines(CKs) expression were detected by either qPCR or ELISA. Possible mechanism involving in- flammatory pathways activation were addressed by western blot. Results: Murine model of UC-like inflammation was successfully established. And we found that BAHD1 existed in the normal internal crypt and surface epithelial cells ubiquitously. Compared with control group, BAHD1 expression in colon tissue were significantly decreased in both UC patients and mice model. In the vitro model system, we found that the protein level of BAHD1 was decreased in the stimulated Caco-2 cells. In addition, consistent with mRNA level of associated CKs enhanced in the siBAHD1 group within stimulatory factors, the interference group secreted more IL-6 and MCP-1 contents in the culture supernatant. As for potential mechanisms of BAHD1 in colitis, increased expression of TNFR1 was found in Caco-2 cells pre-treated with siBAHD1 in gut inflammation model, accompanying with the activation of IKK/ NF-κB and JNK/AP-1 pathways. Conclusions: Collectively, those findings provide evidence that BAHD1 might act as an indispensable safeguard to keep intestine immunological homeostasis.
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Zuo, Chaohui,Xia, Man,Liu, Jingshi,Qiu, Xiaoxin,Lei, Xiong,Xu, Ruocai,Liu, Hanchun,Li, Jianliang,Li, Yongguo,Li, Qinglong,Xiao, Hua,Hong, Yuan,Wang, Xiaohong,Zhu, Haizhen,Wu, Qunfeng,Burns, Michael,Li Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.1
Objectives: Intrahepatic recurrence is the major cause of death among patients with hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) after curative surgical resection. Several approaches have been reported to decrease the recurrence rate. The objective of our study was to compare the clinical effects of transcatheter arterial chemoembolization (TACE) combined with interferon-alpha (IFN-${\alpha}$) therapy on recurrence after hepatic resection in patients with HBV-related HCC with that of TACE chemotherapy alone. Methods: We retrospectively analyzed the data from 228 patients who were diagnosed with HBV-related HCC and underwent curative resection between January 2001 to December 2008. The patients were divided into TACE (n = 126) and TACE-IFN-${\alpha}$ (n = 102) groups for postoperative chemotherapy. The TACE regimen consisted of 5-fluorouracil (5-FU), cisplatin (DDP), and the emulsion mixed with mitomycin C (MMC) and lipiodol. The recurrence rates, disease-free survival (DFS), overall survival (OS), and risk of recurrence were evaluated. Results: The clinicopathological parameters and adverse effects were similar between the 2 groups (P > 0.05). The median OS for the TACE-IFN-${\alpha}$ group (36.3 months) was significantly longer than that of the TACE group (24.5 months, P < 0.05). The 3-and 5-year OS for the TACE-IFN-${\alpha}$ group were significantly longer than those of the TACE group (P < 0.05) and the recurrence rate was significantly lower (P < 0.05). The TACE and IFN-${\alpha}$ combination therapy, active hepatitis HBV infection, the number of tumor nodules, microvascular invasion, liver cirrhosis, and the BCLC stage were independent predictors of OS and DFS. Conclusions: The use of the TACE and IFN-${\alpha}$ combination chemotherapy after curative hepatic resection safely and effectively improves OS and decreases recurrence in patients with HBV-related HCC who are at high risk. Our findings can serve as a guide for the selection of postoperative adjuvant chemotherapy for patients with HBV-related HCC who are at high risk of recurrence.
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The Role of Short Chain Fatty Acids in Irritable Bowel Syndrome
Wenxi Jian,Jiali Wu,Shefeng Zhu,Linying Xin,Chaohui Yu,Zhe Shen 대한소화기 기능성질환∙운동학회 2022 Journal of Neurogastroenterology and Motility (JNM Vol.28 No.4
Irritable bowel syndrome (IBS) is a functional gastrointestinal disorder that is characterized by abdominal pain and disordered bowel habits. The etiology of IBS is multifactorial, including abnormal gut-brain interactions, visceral hypersensitivity, altered colon motility, and psychological factors. Recent studies have shown that the intestinal microbiota and its metabolites short chain fatty acids (SCFAs) may be involved in the pathogenesis of IBS. SCFAs play an important role in the pathophysiology of IBS. We discuss the underlying mechanisms of action of SCFAs in intestinal inflammation and immunity, intestinal barrier integrity, motility, and the microbiota-gut-brain axis. Limited to previous studies, further studies are required to investigate the mechanisms of action of SCFAs in IBS and provide more precise therapeutic strategies for IBS.
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