Predictors and Dynamic Nomogram to Determine the Individual Risk of Malignant Brain Edema After Endovascular Thrombectomy in Acute Ischemic Stroke

Qian-mei Jiang,Shuai Yu,Xiaofeng Dong,Huai-shun Wang,Jie Hou,Zhi-chao Huang,Zhi-liang Guo,Shou-jiang You,Guo-dong Xiao 대한신경과학회 2022 Journal of Clinical Neurology Vol.18 No.3

Background and Purpose This study aimed to construct an optimal dynamic nomogram for predicting malignant brain edema (MBE) in acute ischemic stroke (AIS) patients after endovascular thrombectomy (ET). Methods We enrolled AIS patients after ET from May 2017 to April 2021. MBE was defined as a midline shift of >5 mm at the septum pellucidum or pineal gland based on follow-up computed tomography within 5 days after ET. Multivariate logistic regression and LASSO (least absolute shrinkage and selection operator) regression were used to construct the nomogram. The area under the receiver operating characteristic curve (AUC) and decisioncurve analysis were used to compare our nomogram with two previous risk models for predicting brain edema after ET. Results MBE developed in 72 (21.9%) of the 329 eligible patients. Our dynamic web-based nomogram (https://successful.shinyapps.io/DynNomapp/) consisted of five parameters: basal cistern effacement, postoperative National Institutes of Health Stroke Scale (NIHSS) score, brain atrophy, hypoattenuation area, and stroke etiology. The nomogram showed good discrimination ability, with a C-index (Harrell’s concordance index) of 0.925 (95% confidence interval=0.890–0.961), and good calibration (Hosmer-Lemeshow test, p=0.386). All variables had variance inflation factors of <1.5 and tolerances of >0.7, suggesting no significant collinearity among them. The AUC of our nomogram (0.925) was superior to those of Xiang-liang Chen and colleagues (0.843) and Ming-yang Du and colleagues (0.728). Conclusions Our web-based dynamic nomogram reliably predicted the risk of MBE in AIS patients after ET, and hence is worthy of further evaluation.

Robust Stability Analysis of Time-varying Delay Systems via an Augmented States Approach

Chao-Yang Dong,Ming-Yu Ma,Qing Wang,Si-Qian Ma 제어·로봇·시스템학회 2018 International Journal of Control, Automation, and Vol.16 No.4

This paper focuses on the robust stability analysis of linear systems with time varying delays. The second derivative of the state is used to construct a novel Lyapunov-Krasovskii functional (LKF). Then, both the integrals and derivatives of the states along with the delayed states could be taken into consideration in handling of the LKF. We introduce these augmented variables and establish correlations between them. Moreover, our approach makes it available that quadratic convex combination can be applied not only to the time delay, but also to the derivative of it. Robust stability criteria are presented, and numerical examples illustrate that the methods are less conservative.

Regulatory Mechanisms of Annexin-Induced Chemotherapy Resistance in Cisplatin Resistant Lung Adenocarcinoma

Wang, Chao,Xiao, Qian,Li, Yu-Wen,Zhao, Chao,Jia, Na,Li, Rui-Li,Cao, Shan-Shan,Cui, Jia,Wang, Lu,Wu, Yin,Wen, Ai-Dong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.7

Adenocarcinoma of lung has high incidence and a poor prognosis, woith chemotherapy as the main therapeutic tool, most commonly with cisplatin. However, chemotherapy resistance develops in the majority of patients during clinic treatment. Mechanisms of resistance are complex and still unclear. Although annexin play important roles in various tumor resistance mechanisms, their actions in cisplatin-resistant lung adenocarcinoma remain unclear. Preliminary studies by our group found that in cisplatin-resistant lung cancer A549 cells and lung adenocarcinoma tissues, both mRNA and protein expression of annexins A1, A2 and A3 is increased. Using a library of annexin A1, A2 and A3 targeting combined molecules already established by ourselves we found that specific targeting decreased cisplatin-resistance. Taken together, the underlined effects of annexins A1, A2 and A3 on drug resistance and suggest molecular mechanisms in cisplatin-resistant A549 cells both in vivo and in vitro. Furthermore, the study points to improved research on occurrence and development of lung adenocarcinoma, with provision of effective targets and programmes for lung adenocarcinoma therapy in the clinic.

Isolation and Identification of Lipopeptide Antibiotics from Paenibacillus elgii B69 with Inhibitory Activity Against Methicillin-Resistant Staphylococcus aureus

Rui Ding,Chao-Dong Qian,Yi Teng,Ou Li,Zha-Jun Zhan,Yu-Hua Zhao,Xue-Chang Wu 한국미생물학회 2011 The journal of microbiology Vol.49 No.6

Two lipopeptide antibiotics, pelgipeptins C and D, were isolated from Paenibacillus elgii B69 strain. The molecular masses of the two compounds were both determined to be 1,086 Da. Mass-spectrometry, amino acid analysis and NMR spectroscopy indicated that pelgipeptin C was the same compound as BMY-28160, while pelgipeptin D was identified as a new antibiotic of the polypeptin family. These two peptides were active against all the tested microorganisms, including antibiotic-resistant pathogenic bacterial strains such as methicillin-resistant Staphylococcus aureus (MRSA). Time-kill assays demonstrated that pelgipeptin D exhibited rapid and effective bactericidal action against MRSA at 4×MIC. Based on acute toxicity test, the intraperitoneal LD50 value of pelgipeptin D was slightly higher than that of the structurally related antimicrobial agent polymyxin B. Pelgipeptins are highly potent antibacterial and antifungal agents, particularly against MRSA, and warrant further investigation as possible therapeutic agents for bacteria infections resistant to currently available antibiotics.

Characterization and function of a short peptidoglycan recognition protein from the Chinese oak silkworm, Antheraea pernyi

Li-Shang Dai,Cen Qian, LeiWang,Guo-Qing Wei,Qiu-Ning Liu,Yu Sun,Cong-Fen Zhang,Jun Li,Dong-Ran Liu,Bao-Jian Zhu,Chao-Liang Liu 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.4

Peptidoglycan recognition proteins (PGRPs) are conserved proteins in animals from insects to mammals and play an important role in immune response by recognizing peptidoglycan on microbial surfaces. In this study, a PGRP gene from Antheraea pernyi, named Ap-PGRP-A, was isolated and characterized. Sequence analysis revealed that the full-length cDNA of Ap-PGRP-A was 961 bp, containing a 40 bp 5′-untranslated sequence, a 339 bp 3′-untranslated region and an open reading frame of 582 bp. This gene encoded a putative protein of 193 amino acid residues. Phylogenetic analysis indicated that Ap-PGRP-A had the closest protein sequence similarity to Antheraea mylitta PGRP. The recombinant protein was successfully expressed in Escherichia coli cells and a rabbit anti-Ap-PGRP-A antibody was also prepared. Real-time quantitative reverse transcription-PCR analysis showed that Ap-PGRP-A was extensively expressed in the fat body, midgut, hemocyte,malpighian tubule and epidermis of A. pernyi, especially in the fat body and midgut. The expression levels of Ap-PGRP-A were significantly up-regulated by three types of microorganisms, and Ap-PGRP-A expression was more sensitive in response to the Gram-negative bacterium E. coli than the Gram-positive bacterium Bacillus subtilis or the fungus Beauveria bassiana. These data indicate that Ap-PGRP-A may play a role in the innate immune responses of A. pernyi.

Numerical analysis of unstable turbulent flows in a centrifugal pump impeller considering the curvature and rotation effect

Weixiang Ye,Zu-chao Zhu,Zhong-Dong Qian,Xianwu Luo 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.7

In this study, a modified partially averaged Navier–Stokes model (MSST PANS) is proposed by treating a modified shear stress transport (SST) k–ω model as the parent turbulence model. The unstable turbulent flow in a centrifugal pump that considers the curvature and rotation effect is investigated as the test case to evaluate the performance of the MSST PANS model and analyze the flow instability in a centrifugal pump. The SST k–ω and the standard k–ε PANS models are also evaluated for comparison. Results show that the MSST PANS model exhibits excellent performance and delivers the most satisfactory prediction results of the positive slope of the characteristic curve, time-averaged internal flows, and velocity profiles. The energy loss based on the energy balance equations is adopted to provide an explanation of the internal flow evolutions in pumps. The findings also indicate energy loss distribution is associated with the positive slope phenomenon. The high-velocity gradient flows at the entrance of the blade-toblade passage and the reverse flows at the impeller exit are the main reasons for the high turbulent kinetic energy in the impeller. The MSST PANS model demonstrates promising applications in the field of hydraulic machinery, where unstable turbulent flows are prevalent.

Isolation and Identification of Newly Isolated Antagonistic Streptomyces sp. Strain AP19-2 Producing Chromomycins

Wu, Xue-Chang,Chen, Wei-Feng,Qian, Chao-Dong,Li, Ou,Li, Ping,Wen, Yan-Ping The Microbiological Society of Korea 2007 The journal of microbiology Vol.45 No.6

A new antagonistic strain of actinomycete, designated AP19-2, was isolated from the feces of giant pandas inhabiting the Foping National Nature Reserve in China. Cultural characteristic studies strongly suggested that this strain is a member of the genus Streptomyces. The nucleotide sequence of the 16S rRNA gene of strain AP19-2 evidenced profound similarity (97-99 %) with other Streptomyces strains. Two pure active molecules were isolated from a fermentation broth of Streptomyces sp. strain AP19-2 via extraction, concentration, silica gel G column chromatography, and HPLC. The chemical structures of the two related compounds (referred to as chromomycin $A_2$ and chromomycin $A_3$) were established on the basis of their Infrared spectra (IR), High Resolution Electrospray Ionization Mass Spectrometry (HR-ESI-MS), and Nuclear Magnetic Resonance (NMR) data, and by comparison with published data.

Isolation and Identification of Newly Isolated Antagonistic Streptomyces sp. Strain AP19-2 Producing Chromomycins

Xue-Chang Wu,Wei-Feng Chen,Chao-Dong Qian,Ou Li,Ping Li,Yan-Ping Wen 한국미생물학회 2007 The journal of microbiology Vol.45 No.6

A new antagonistic strain of actinomycete, designated AP19-2, was isolated from the feces of giant pandas inhabiting the Foping National Nature Reserve in China. Cultural characteristic studies strongly suggested that this strain is a member of the genus Streptomyces. The nucleotide sequence of the 16S rRNA gene of strain AP19-2 evidenced profound similarity (97-99%) with other Streptomyces strains. Two pure active molecules were isolated from a fermentation broth of Streptomyces sp. strain AP19-2 via extraction, concentration, silica gel G column chromatography, and HPLC. The chemical structures of the two related compounds (referred to as chromomycin A2 and chromomycin A3) were established on the basis of their Infrared spectra (IR), High Resolution Electrospray Ionization Mass Spectrometry (HR-ESI-MS), and Nuclear Magnetic Resonance (NMR) data, and by comparison with published data.