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정식 시기와 적심 횟수가 화단국화의 생장과 개화에 미치는 영향
姚婧超, 정해준, 김동찬, 이진희, 권민훈, 서병기 배재대학교 자연과학연구소 2013 自然科學論文集 Vol.24 No.1
This study was conducted to investigate optimum planting date and pinching times on the growth and flowering of garden chrysanthemum(Dendranthema grandiflorum Ramat.). 3 lines of garden mums were planted on May 16, June 13 and July 11 respectively. As planting date was delayed, all the 3 lines' plant height and plant width was reduced. 09-19-49 line got the biggest plant height and width, followed by 09-11-37 line. And 08-166-02 line was the smallest. In different planting times, the experiment group of May 16 and June 13 got the biggest plant height, plant width and stem diameter. However, in the experiment group of May 16, most of the plants got flattened. The experiment group of June 13 got the best ornamental value, and the ornamental value was reduced in the experiment group of July 11 by the short vegetative growth. 09-19-49 line got the most flowers, while in different planting time, the experiment group of May 16 got the most flowers. 09-11-37 line got the biggest flower diameter. 09-11-37 line and 09-09-51 line were planted in the containers on the rooftop. Each groups were pinched by 1, 2 and 3 times on July 2, July 26 and Aug 16 respectively. There was no effect on plant height, plant width, and stem diameter of 09-09-51 line and 09-11-37 line between 1-time-pinching group and 2-time-pinching group. But 3-time-pinching group was reduced. There was no significance in flower diameter of these two lines but the number of flowers was reduced by more pinching times. Therefore, in this experiment, the groups which were planted on June 13 got the best ornamental value and the recommended planting time is in the middle of June. Furthermore, 2 times of pinching was recommended.
Natural gas hydrate occurrence and detection in the Sea of Okhotsk
진영근(Jin, Young-Keun),CHAOS Scientific Party, CHAOS 한국신재생에너지학회 2006 한국신재생에너지학회 학술대회논문집 Vol.2006 No.11
The Sea of Okhotsk is the unique area providing the highest methane production rate of the northern hemisphere. The area of focused fluid venting offshore the NE Sakhalin continental slope was investigated during the CHAOS (Hydro-Carbon Hydrate Accumulations in the Okhotsk Sea) expeditions onboard of RV Akademik Lavrentyev In 2003, 2005 and 2006. The International Research Project CHAOS (Russia-Korea-Japan) aimed at the study of gas hydrate formation processes associated with the fluid venting in the Sea of Okhotsk. Several new gas hydrate accumulations were discovered during the cruise. Hydrate-associated structures have been named as KOPRI, VNIIOKeangeologia, POI and KIT (the names of cruise participant institutes) Some of hydrate-bearing cores contain big amount of gas hydrates: massive gas hydrate layers (up to 35cm thick) were recovered. The shallowest submarine gas hydrate accumulations in the world (at the depth less then 400m) were discovered during the cruise.
Effect of white, red and black ginseng on physicochemical properties and ginsenosides.
Jin, Yan,Kim, Yeon-Ju,Jeon, Ji-Na,Wang, Chao,Min, Jin-Woo,Noh, Hae-Yong,Yang, Deok-Chun Kluwer Academic 2015 Plant foods for human nutrition Vol.70 No.2
<P>A systematic comparison of the ginsenosides and physicochemical properties of white ginseng (WG), red ginseng (RG) and black ginseng (BG) was performed. The purpose of the present study was to identify the effects of the physicochemical properties by steaming process. During the steaming process, ginsenosides transform into specific ginsenosides by hydrolysis, dehydration and isomerization at C-3, C-6 or C-20. Steaming ginseng led to a significant increase in reducing sugar, acidic polysaccharide and phenolic compounds content. Antioxidative properties were investigated using the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, compared with BHA (Butylated hydroxyanisole). RG and BG exhibited higher antioxidant activity than WG. The maximum residue level for Benzo(a)pyrene was established to 5 μg/kg in food products. The levels of benzo(a)pyrene in WG and RG were not detected. Benzo(a)pyrene was detected in the BG, the content was 0.17 μg/kg. The scientific achievements of the present study could help consumers to choose different type of ginseng products available on the market.</P>
Jin, Yan,Kim, Yeon-Ju,Hoang, Van-An,Young Jung, Sun,Nguyen, Ngoc-Lan,Woo Min, Jin,Wang, Chao,Yang, Deok-Chun Institute of Applied Microbiology, University of T 2014 The Journal of general and applied microbiology Vol.60 No.2
<P>The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain DCY69(T) is JX233806. A Gram-reaction-negative, oxidase- and catalase-positive, non-gliding motile strain, designated strain DCY69(T), was isolated from the soil of a ginseng field in the Republic of Korea. Colonies of strain DCY69(T) were circular, 0.5-1.5 mm diameter, yellow, and convex on an R2A agar plate after 2 days. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain DCY69(T) belonged to the genus Flavobacterium with 90.5-98.3% gene sequence similarity. The major predominant quinone was MK-6. The major cellular fatty acids were iso-C15:0, iso-C17:0 3-OH, iso-C15:0 3-OH and summed feature 3 (containing C16:1ω7c and/or C16:1ω6c). The major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and unidentified polar lipids (L1, L2). The genomic DNA G+C content of strain DCY69(T) was 35.0mol%. The strain DCY69(T) transformed ginsenoside Rb1 into Rd and F2. Based on the polyphasic taxonomic data, strain DCY69(T) is considered to represent a novel species of the genus Flavobacterium, for which the name Flavobacterium panaciterrae sp. nov. is proposed. The type strain is DCY69(T)(= KCTC 32392(T) = JCM 19161(T)), isolated from the soil of a ginseng field in the Republic of Korea.</P>
Wang, Chao,Park, Jin Hyuk,Tan, Songwen,Cui, Chunzhi,Jin, Jing-Yi,Ahn, Dong June Hindawi Limited 2017 Journal of nanomaterials Vol.2017 No.-
<P>Polydiacetylenic complex films were prepared using 10,12-pentacosadiynoic acid (PCDA) and para-xylenediamine (<I>p</I>XDA) upon acid-base interactions. The thermochromatic reversibility of the complex films was modulated by changing the mixed molar ratio (3 : 1, 2 : 1, and 1 : 1) of the two molecules. The corresponding conformational changes of the complex films were studied by ex situ FTIR analysis upon thermal stimulation for the first time. In addition, the binding specificities of <I>α</I>-, <I>β</I>-, and <I>γ</I>-cyclodextrins (CDs) with the films were studied, where the <I>α</I>-CDs can induce stronger red fluorescent emission of the films. These fundamental results may be useful for platforms that use these polydiacetylenic complex films as optoelectronic devices or chemical/biological sensors.</P>
( Qing Chao Jin ),( Hua Li Yin ),( Xiao Wei Hong ),( Zhi Hua Jin ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.6
A gene related to high pristinamycin yield in Streptomyces pristinaespiralis was selected by amplified fragment length polymorphism (AFLP) and its functions were investigated by gene disruption. First, a 561 bp polymorphic sequence was acquired by AFLP from high-yield recombinants compared with the S. pristinaespiralis ancestor ATCC25486, indicating that this approach is an effective means of screening for valuable genes responsible for antibiotic yield. Then, a 2,127 bp open reading frame of a gene designated spy1 that overlaps with the above fragment was identified and its structure and biological functions were investigated. In silico analysis of spy1 encoding a deduced 708-amino-acid-long serine/threonine protein kinase showed that it only contains a catalytic domain in the N-terminal region, which is different from some known homologs. Gene inactivation of chromosomal spy1 indicated that it plays a pleiotropic regulatory function in pristinamycin production, with a positive correlation to pristinamycin I biosynthesis and a negative correlation to pristinamycin II biosynthesis.
RGS2 promotes estradiol biosynthesis by trophoblasts during human pregnancy
Tang Chao,Jin Meiyuan,Ma Bingbing,Cao Bin,Lin Chao,Xu Shouying,Li Jiayong,Xu Qiang 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-
Production of estradiol (E2) by the placenta during human pregnancy ensures successful maintenance of placental development and fetal growth by stimulating trophoblast proliferation and the differentiation of cytotrophoblasts into syncytiotrophoblasts. Decreased levels of E2 are closely associated with obstetrical diseases such as preeclampsia (PE) in the clinic. However, the mechanisms underlying the inhibition of placental E2 biosynthesis remain poorly understood. Here, we report that regulator of G-protein signaling 2 (RGS2) affects E2 levels by regulating aromatase, a rate-limiting enzyme for E2 biosynthesis, by using human trophoblast-derived JEG-3 cells and human placental villus tissues. RGS2 enhanced the protein degradation of the transcription factor heart and neural crest derivatives expressed 1 (HAND1) by suppressing ubiquitin-specific protease 14 (USP14)-mediated deubiquitination of HAND1, resulting in the restoration of HAND1-induced trans-inactivation of the aromatase gene and subsequent increases in E2 levels. However, aromatase bound to RGS2 and repressed RGS2 GTPase activating protein (GAP) activity. Moreover, we observed a positive correlation between RGS2 and aromatase expression in clinical normal and preeclamptic placental tissues. Our results uncover a hitherto uncharacterized role of the RGS2-aromatase axis in the regulation of E2 production by human placental trophoblasts, which may pinpoint the molecular pathogenesis and highlight potential biomarkers for related obstetrical diseases.
Yan-chao Wang,Jin-miao Lu,Hui-zi Jin,Ai-niu Ma,Jin-yang Zhang,Nian Gong,Qi Xiao,Bin Zhu,Ying-fang Lv,Na Yu,Wei-dong Zhang,Yong-xiang Wang 한국영양학회 2014 Nutrition Research and Practice Vol.8 No.2
BACKGROUND: Groundwater is believed to possess many beneficial effects due to its natural source of various minerals. In this study, we examined the effects of natural Jeju groundwater S1 (SamdasooTM), S2 and S3 pumped up from different locations of Jeju Island, Korea, along with local tap water, on body weight gain, serum lipids and lipoproteins, and liver histopathology in high-fat diet-induced hyperlipidemic rats. MATERIALS/METHODS: Rats were randomly and equally divided into 6 groups. Different water samples were supplied to the hyperlipidemic rats as their daily drinking water and the widely-used anti-hyperlipidemic drug simvastatin was used as a positive control. Body weight, serum lipids and lipoproteins were measured weekly. Liver weight, liver index and liver histopathology were examined after the execution of the rats. RESULTS: After drinking Jeju groundwaters for two months, S2 but not S3 significantly reduced weight growth and serum triglycerides levels and increased high density lipoprotein-C (HDL-C) without affecting total cholesterol or LDL-C. S1 and particularly S2 significantly reduced the severity of liver hypertrophy and steatosis. All Groundwaters had much higher contents of vanadium (S3>S2>S1>>tap water) whereas S1 and S2 but not S3 markedly blocked autoxidation of ferrous ions. CONCLUSION: Jeju Groundwater S1 and particularly S2 exhibit protective effects against hyperlipidemia and fatty liver and hypothesize that the beneficial effect of Jeju Groundwaters may be contributed from blockade of autoxidation of ferrous ions rather than their high contents of vanadium.