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구본재 ( Bon Jae Gu ),류기형 ( Gi Hyung Ryu ) 한국산업식품공학회 2011 산업 식품공학 Vol.15 No.2
The objective of this study was to determine the effect of die geometry on expansion index of extruded corn flour. Water solubility index, water absorption index and specific mechanical energy (SME) input were analyzed to observe the relationship with die geometry. The feed moisture content was 20 and 25%. Die dimensions were tapered angle (57, 95°) and length/diameter (L/D) ratio of die land (0.67, 1.67 and 2.67). The SME input was the highest at 20% moisture content and 2.23E-10 m3 die constant. The sectional and volumetric expansion indices at 20% moisture were increased with increase in die constant. However, die constant did not influence sectional expansion index of corn flour extrudate at 25% moisture content. The extruded corn flour at 25% moisture content had higher longitudinal expansion index than those of extruded corn flour at 20% moisture content. Sectional expansion and longitudinal expansion index were negatively correlated. The water absorption index and water solubility index were not affected with the die constant.
사출구 구조와 탄산가스 주입이 옥수수 압출성형물의 물리적 특성에 미치는 영향
구본재 ( Bon Jae Gu ),류기형 ( Gi Hyung Ryu ) 한국산업식품공학회 2012 산업 식품공학 Vol.16 No.2
The objective of this study was to determine the effect of die geometry and carbon dioxide injection on the physical properties of extruded corn flour. The extrusion variables were melt temperatures of 95 and 110oC; carbon dioxide gas flow rates of 0, 100 and 200 mL/min. In die dimension, the length was 2 or 5 mm and the tapered angle of die hole was 57 or 95o. The specific mechanical energy (SME) input was the lowest at 110oC melt temperature, 200 mL/min carbon dioxide gas flow rate and 3.36×10-10 m3 die constant. The sectional expansion of corn flour extrudate was higher at 57o than that at 95o tapered angle. Also, the sectional expansion was increased with increasing carbon dioxide injection flow rate from 0 mL/min to 200 mL/min. The specific length was increased with increasing carbon dioxide injection rate. The density, breaking strength and apparent elastic modulus were lower in the low die constant. Extruded corn flour at 95o tapered angle had more pores than one at 57o tapered angle.
In Bon Goo,Jae Hyun Im,Hyun Woo Gil,Sang Gu Lim,In-Seok Park 한국발생생물학회 2015 발생과 생식 Vol.19 No.3
The influence of triploidization on cell and nucleus size characteristics of the same tissues of erythrocyte, retina, kidney, hepatocyte and midgut epithelium in marine medaka, Oryzias dancena has been determined histologically. Induced triploid fish are produced by cold shock treatments. Likewise, the size of horizontal cell nucleus in inner nuclear layer of retina, ganglion cell nucleus in ganglion cell layer of retina, proximal tubule cell of kidney, hepatocytes and nuclear height of midgut epithelium all appear to be significantly larger than diploid (P<0.05). On the other hand, retina thickness is larger in diploid than induced triploid (P<0.05). Induced triploid shows low density of cell number. Results of this study suggest that same characteristics in the induced triploid exhibiting larger cells and nucleus sizes with fewer number of cells than the diploid can be useful criteria for the distinction between diploid and induced triploid, and also the ploidy level in marine medaka.
Potential Anti-photoaging Effect of Amino Sugar
( Bon Geun Koo ),( Gu Baek ),( Yeon Woo Kim ),( Ji Min Park ),( Min Ju Kim ),( Hyun Seo Ko ),( Jae Kweon Park ) 한국키틴키토산학회 2012 한국키틴키토산학회지 Vol.17 No.2
The anti-photoaging activity of amino sugar was measured by monitoring the survival rate toward a bacterial species Serratia marcescens. Cells incubated in liquid and solid culture medium containing various sugars were irradiated under UV 254 nm or 365 nm. Among sugars tested in this study, relatively high photo-protective activity in the presence of amino sugars such as glucosamine, mannosamine, and galactosamine on cells irradiated under UV 365 nm were observed incubated in liquid culture medium compared to other neutral sugars tested. Especially, glucosamine has displayed the highest photo-protective activity (75%) estimated based on the growth recovery of cells irradiated under UV 365 nm. On the other hands, neutral sugar including glucose, mannose and galactose showed the marginal photo-protective effect on cells irradiated under UV 365 nm. However, both amino sugars and neutral sugars displayed neither photo-protective activities on cells incubated in liquid nor solid agar medium under UV 254 nm. Our results indicate that amino sugars has shown selective absorbing activities toward UV 365 nm and are useful as potentially bioactive anti-photoaging materials in cosmeceutical and pharmaceutical industries.
Evaluation of Antioxidative Activities of Polymeric Carbohydrates
( Bon Geun Koo ),( Gu Baek ),( Jin Young Park ),( Ji Min Park ),( Min Ju Kim ),( Hyun Seo Ko,),( Byung Jae Ahn ),( Jae Kweon Park ) 한국키틴키토산학회 2012 한국키틴키토산학회지 Vol.17 No.3
The antioxidant capabilities of various polysaccharides including alginate, β-glucan, chitosan, fucoidan and hyaluronate were investigated using three established assays: 2,2-diphenyl-1-picryhydrazyl (DPPH) radical scavenging, ferric reducing antioxidant power (FRAP), and superoxide dismutases (SODs) which are metalloenzymes that catalyze the dismutation of the superoxide anion to hydrogen peroxide and molecular oxygen. The DPPH radical scavenging assay revealed that among the five polysaccharides tested in this study, hyaluronic acid had relatively high antioxidant activity. However, low molecular weight chitooligosaccharides, consisting of glucosamine (GlcN) n, n = 6-9, exhibited slightly higher radical scavenging activity than hyaluronic acid. Interestingly, no DPPH radical scavenging activity was observed from the high molecular weight chitosan. On the contrary, in the FRAP activity measurements, high molecular weight chitosan showed the significant level of activity compared to the chitooligosaccharides, which has shown the similar activity of fucoidan and hyaluronic acid, although it was too marginal. Subsequently, fucoidan has shown the most significant antioxidant activity in SOD assay. These results suggest that antioxidant activity obviously depends on the molecular weight and their structural component as a key factor involved in each reaction mechanisms.
Bon-Geun Koo,Gu Baek,Min Jung Kim,Seo Yoon Shin,Yong Il Park,Jae Kweon Park 한국당과학회 2011 한국당과학회 학술대회 Vol.2011 No.1
Chitinolytic and chitosanolytic microorganisms are widely distributed in nature. Biological and enzymatic activities of these enzymes are applied to produce hydrolytic oligosaccharides which have various biological functions involved in anticancer, antimicrobial activities, immune-stimulating activities and so on. In the present study, we describe the biological characteristics including the diversity based on 16S rDNA sequence analysis, production of chitosanase and its hydrolysates, and enzyme activities. Among 15 species of chitosanolytic microorganisms isolated in this study, one named as Bacillus subtilis JH-1122 based on 16s rDNA sequence homology search has shown the highest similarity with most of sub-species of Bacillus. Various sizes of chitosan hydrolysates (Low Molecular Weight Chitosan, LMWC) ranging from 3 to 60 kDa were prepared to assess the effect of molecular weight on antimicrobial activity by enzymatic digestion of chitosan using chitosanase isolated and partially purified from the culture medium of Bacillus subtillis JH-1122. Especially it has shown the higher antimicrobial activity toward antibiotics resistant microorganisms such as Escherichia coli, methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa. These results suggest that LMWC may be a predominant factor either directly or indirectly affecting on broad antimicrobial activities.