http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
류병호,이병호,박형숙,하미숙,김동석,Ryu, Beung-Ho,Lee, Beung-Ho,Park, Hyeong-Suck,Ha, Mi-Suck,Kim, Dong-Seuk 생화학분자생물학회 1986 한국생화학회지 Vol.19 No.3
말똥 성게를 인공적으로 수정시킨 후, 칼럼 크로마토그래피법으로 분리 정제하여 리보뉴크레아제 $H_1$과 리보뉴크레아제 $H_2$를 얻었다. 리보뉴크레아제 $H_1$의 분자량은 약 68,000이었고, 리보뉴크레아제 $H_2$의 분자량은 약 44,000 이었다. 리보뉴크레아제 $H_1$는 약알카리성에서, 리보뉴크레아제 $H_2$는 알카리성에서 활성이 높았다. 이들 효소는 모두 2가이온인 마그네슘이온 ($Mg^{2+}$), 망간이온($Mn^{2+}$)과 그리고 염의 일정농도에서 활성을 나타내고 있으며, N-에칠 말례이마이드에 감수성이 높았다. 그리고, 리보뉴크레아제 $H_1$과 $H_2$는 단일가닥과 이중가닥의 활성은 없었으나, RNA-DNA hydrid는 급히 저하 시켰다. From the sea-urchin, Hemicentrotus pulcherrimus, we have purified by two column chromatographic steps two ribonuclease H (RNase H) activities of RNase $H_1$ and RNase $H_2$ in homogeneous form. RNase $H_1$ was molecular weight around 68,000 and RNase $H_2$ was around 44,000 by Sephadex G-75 gel filteration and SDS-polyacrylamid gel electrophoresis. RNase $H_1$ was optimal active as well as slightly alkaline pH and RNase $H_2$ was as alkaline pH. These enzymes shown to be a function of the divalent metal ion $Mg^{2+}$, $Mn^{2+}$ and salt employed as activator, also were sensitive -SH reagent N-ethylmaleimide. RNase $H_1$ and RNase $H_2$ were shown no activity with single or double stranded RNA but rapidly hydrolysis the RNA-DNA hybrid.
Characteristics of Bluish Purple Pigment Produced by Streptomyces californicus KS - 89
류병호(Beung-Ho Ryu),지영애(Young-Eh Chi),이병호(Byeong-Ho Lee),박법규(Bub-Gyu Park),박우열(Woo-Yeol Park) 한국식품영양과학회 1990 한국식품영양과학회지 Vol.19 No.3
천연식용 색소의 개발을 위한 하나의 방안으로 Streptomyces californicus KS - 89에 의하여 생산되는 청자색 색소의 물리 화학적 특성을 조사하였다. 청자색 색소를 실리카겔의 칼럼 크로마토그래피에 의하여 분리하였고, C.I.E. chromatic diagram에 의하면 청자색에 속하며 UV 최대 흡수대는 575㎚이였다. 이 색소의 수용액에서의 색조는 pH 5~8 범위내에서는 안정하였고, 자외선에 의하여 영향을 받지 않으나, 때때로 열에 의하여 색상이 약간 흐려진다. 한편 금속염에는 거의 안정하였고, 특히 이 색소는 돌연변이원성과 항암 효과는 없었으며, 또 항생 물질능도 없었음이 밝혀졌다. Aqueous solution pigment produced by Streptomyces californicus KS-89 showed a vivid bluish purple pigment and purified by silica gel column chromatography. The pigment indicated a deep purple color zone by the C.I.E. chromatic diagram, and showed UV absorption maxima at 575㎚. The color intensity in aqueous solution was fairly stable in the ranges of pH 5~8 and was not affected by UV light, however, sometimes it had faded slightly by the heat. It was possible to prevent significantly by the addition of metal salt. Especially, this pigment has no mutagenicity and antitumor activity and it appears to be devoid of antibiotic activity.
Cultural Conditions of Streptomyces californicus KS - 89 for the Production of Bluish Purple Pigment
지영애(Young-Eh Chi),이병호(Byeong-Ho Lee),박우열(Woo-Yeol Park),박법규(Bub-Gyu Park),류병호(Beung-Ho Ryu) 한국식품영양과학회 1990 한국식품영양과학회지 Vol.19 No.3
Streptomyces californicus KS-89 에서 생산되는 청자색 색소를 생산하기 위한 기질별 최적조건을 구하였다. 청자색 색소를 생산할 때 요구되는 기질로서는 탄소원으로 가용성 전분과 glycerol이, 질소원으로 글루타민산소다와 질산나트륨으로서 30℃에서 7일 동안에 생산능이 가장 우수하였다. 이때, 황산철은 필수적인 무기질이었다. 결론적으로 청자색 색소의 생산을 위한 최적 배양조건은 2.0% soluble starch, 1% glycerol, 0.5% sodium glutamate, 0.05% sodium nitrate, 0.001% L-proline, 0.025% K₂HPO₄, 0.005% MgSO₄ㆍ7H₂O, 0.04% FeSO₄ㆍ7H₂O, 0.001% thiamine. HCI과 pH는 7.0이었다. The optimal cultural conditions for production of the bluish purple pigment by the cultivation of Streptomyces californicus KS-89 were determined with various substrates. The carbon and nitrogen sources on the production of pigment indicated that soluble starch and glycerol as carbon sources and sodium glutamate, sodium nitrate as nitrogen sources given a maximum yield of the pigment at 30℃ for 7 days. The addition of ferrous sulfate was essential. The highest production of pigment was observed with cultivation in a medium containing 2.0% soluble starch, 1% glycerol, 0.5% sodium glutamate, 0.05% sodim nitrate, 0.001% L-proline, 0.025% K₂HPO₄, 0.005% MgSO₄ㆍ7H₂O, 0.04% FeSO₄ㆍ7H₂O, 0.001% thiamineㆍHCI and pH 7.0.
하미숙,김동석,류병호,이병호,박형숙 생화학분자생물학회 1991 BMB Reports Vol.19 No.3
From the sea-urchin, Hemicentrotus pulcherrimus, we have purified by two column chromatographic steps two ribonuclease H (RNase H) activities of RNase H₁ and RNase H₂ in homogeneous form. RNase H1 was molecular weight around 68,000 and RNase H₂ was around 44,000 by Sephadex G-75 gel filteration and SDS-polyacrylamid gel electrophoresis. RNase H₁ was optimal active as well as slightly alkaline pH and RNase H₂ was as alkaline pH. These enzymes shown to be a function of the divalent metal ion Mg^(2+), Mn^(2+) and salt employed as activator, also were sensitive -SH reagent N-ethylmaleimide. RNase H₁ and RNase H₂ were shown no activity with single or double stranded RNA but rapidly hydrolysis the RNA-DNA hybrid.