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Inhibitory Effects of Silsosangami on the Platelet Aggregation
Kim, Jong Soo,Kim, Beob Jin,Kim, Han Geu,Ahan, Jong Chan,Lee, Soo Kyung,Chung, Tae Wook,Choi, Dall Yeong,Kim, Cherl Ho,Park, Won Hwan 대한동의생리학회,대한동의병리학회 2002 동의생리병리학회지 Vol.16 No.4
The thrombosis importantly came to the front as the risk factor of these circulation system's disease. SilsoSanGami(SSG) was used for investigating the inhibitory effect on platelet-activating factor-induced platelet aggregation about drugs that used to improvement various symptoms created by the thrombosis in oriental medicine. In this study, the water-extracted SSG was investigated for its possible antithrombotic action on platelets. The antithrombotic activity of water-extracted SSG was deduced from its ability to suppress platelet aggregation, ATP-exocytosis, and the generation of prostaglandin E_2 and thromboxane A_2 by human platelets, stimulated with arachidonic acid. Water-extracted SSG dose-dependently suppressed the aggregation of human platelets, the release of endogenous ATP, and the formation of PGE_2 and TXB_2, both the latter usually detected to estimate the activity of COX and TXS, respectively. Since the IC_50 values necessary to inhibit COX (115 ㎍/㎖ SSG) and TXS(74 ㎍/㎖ SSG) were in the same range, inhibition of COX is suggested to be the primary target of water-extracted SSG, thus suppressing the formation of PGE_2 which is metabolized by TXS to TXA_2. We considerated that SSG has practical applicational value of clinical trial in the thrombosis caused by platelet aggregation.
김법전(Kim Beob-Jeon),임희원(Lim Hee-Won),김덕성(Kim Deok-Sung),신우철(Shin U-Cheul) 한국태양에너지학회 2018 한국태양에너지학회 논문집 Vol.38 No.4
nZEH (net-Zero Energy House) is defined as a self-sufficient energy building where the sum of energy output generated from new & renewable energy system and annual energy consumption is zero. The electricity generated by new & renewable energy system with the form of distributed generation is preferentially supplied to electrical demand, and surplus electricity is transmitted back to grid. Due to the recent expansion of houses with photovoltaic system and the nZEH mandatory by 2025, the rapid increase of distributed generation is expected. Which means, we must prepare for an electricity-power accident and stable electricity supply. Also electricity charges have to be reduce and the grid-connected should be operated efficiently. The introduction of ESS is suggested as a solution, so the analysis of the load matching and grid interaction is required to optimize ESS design. This study analyzed the load matching and grid interaction by expected consumption behavior using actual data measured in one-minute intervals. The experiment was conducted in three nZEH with photovoltaic system, called all-electric houses. LCF (Load Cover Factor), SCF (Supply Cover Factor) and f grid (Grid Interaction Index) were evaluated as an analysis indicator. As a result, LCF, SCF and f grid of A house were 0.25, 0.23 and 0.27 respectively; That of B house were 0.23, 0.23, 0.19, and that of C were 0.20, 0.19, 0.27 respectively.
신유혈(腎兪穴)의 침자극(鍼刺戟)과 황기약침(黃기藥鍼)이 실험용 생쥐의 면역활성 Cytokine인 IL-4 유전자발현에 미치는 영향
김법진 ( Beob Jin Kim ),박수영 ( Soo Young Park ),김창환 ( Chang Hwan Kim ),김철호 ( Cherl Ho Kim ),문진영 ( Jin Young Moon ),최달영 ( Dall Yeong Choi ),김준기 ( Jun Kee Kim ),박선동 ( Sun Dong Park ),박원환 ( Won Hwan Park ) 대한경락경혈학회 2001 Korean Journal of Acupuncture Vol.18 No.2
Acupuncture and Radix Astragali aqua-acupuncture stimuli have many been used to cure human diseases. However, it still remains to be unknown on its action mechanism, physiolosical and biochemical aspects. Thus, many attempts were made to show the scientific background covering the above mentioned mechanisms. Most recent studies show that these tests improve blood circulatory system and increase leucocyte counts. In this study, we have applied the acupuncture stimuli to mouse Synsu(BL23), which is a stimulative point of oriental medicine, to see if cytokine such as IL-4 can be detected. Mice were treated with lipopolysaccharide(LPS) for inflammation induction, and then reverse transcriptase-polymerase chain reaction (RT-PCR) using each primer set was performed to trace the amounts of mRNA. The results are summarized as follow; 1. IL-4 was expressed in LPS-nontreated mouse at 30min after acupuncture-stimuli, however, hardly expressed in after 1, 2hrs. 2. IL-4 was continuously expressed in LPS-treated mouse at 30min to 3hrs after acupuncture-stimuli, especially highly expressed at 30 to 60min. And IL-4 was a little decreased after 3hrs but was continuosly expressed. 3. IL-4 was highly expressed in LPS-nontreated mouse at 15min after Radix Astragali aqua-acupuncture stimuli, and expression of IL-4 gradually decreased at 30min to 3hrs. 4. IL-4 was gradually expressed in LPS-treated mouse at 30min to 3hrs after Radix Astragali aqua-acupuncture stimuli, especially highly expressed after 1hr. Therefore, IL-4 in LPS-treated mouse was expressed more effectively than that of LPS-nontreated mouse after acupuncture and Radix Astragali aqua-acupuncture stimuli. And in LPS-treated mouse IL-4 after Radix Astragali aqua-acupuncture stimuli was expressed more effectively than after acupuncture stimuli. On the other hand, now, we are in progress to elucidate the immunological action mechanism of acupuncture and Radix Astragali aqua-acupuncture stimuli. And cytokine IL-4 can be used not only basis of the effect of acupuncture and Radix Astragali aqua-acupuncture but a diagnostic guide through the immunological actions of those.
GO-08 : Overexpression of SOCS 1/3 May Inhibit Invasion and Metastasis of Cervical Cancer
( Moon Hong Kim ),( Won Woo Kim ),( Miae Kang ),( Beob Jong Kim ),( Seok Cheol Choi ),( Sang Young Ryu ),( Jae Hoon Jeong ) 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.99 No.-
To investigate whether the overexpression of SOCS-1/3 affects the radiosensitivity and to find the association between the radiosensensitivity and COX-2, p21, MMP-2. We established stable SOCS-overexpressing HeLa cell line with pLNCX2-mSOCS1- Myc and pLNCX2-mSOCS3-FLAG. Also we established xenograft nude mouse model for tumor growth delay experiment. FACS and immunohistochemistry were employed to elucidate the pattern of expression of COX-2, MMP-2, p21, and tunnel staining for apoptosis evaluation in SOCS-1/3 overexpressing HeLa cells. Migration inhibition assay was performed to find whether SOCS overexpression affected cell migration. SOCS-1/3 overexpression induced p21 protein and affected cell cycle regulation. We observed SOCS-1/3 overexpression caused G2/M arrest after 5 Gy irradiation. Also we found that SOCS-1/3 overexpression inhibited cell migration. In xenograft mouse model, tumor growth delay was observed in SOCS overexpressing tumor compared to wild type tumor (HeLa). Immunohistochemistry showed that COX-2 expression had no difference while MMP-2 expression was decreased in SOCS-1 overexpressing tumor. Also p21 expression increased strongly in SOCS-1 overexpressing tumor. Also increased apoptotic activity was observed in SOCS-1 overexpressin tumor through tunnel staining. SOCS-1/3 overexpression may inhibit invasion and metastasis of cervical cancer.
Mun, Se Hwan,Kim, Hyuk Soon,Kim, Jie Wan,Ko, Na Young,Kim, Do Kyun,Lee, Beob Yi,Kim, Bokyung,Won, Hyung Sik,Shin, Hwa-Sup,Han, Jeung-Whan,Lee, Hoi Young,Kim, Young Mi,Choi, Wahn Soo The Japanese Pharmacological Society 2009 Journal of pharmacological sciences Vol.111 No.1
<P>We investigated whether oral administration of curcumin suppressed type II collagen–induced arthritis (CIA) in mice and its effect and mechanism on matrix metalloproteinase (MMP)-1 and MMP-3 production in CIA mice, RA fibroblast-like synoviocytes (FLS), and chondrocytes. CIA in mice was suppressed by oral administration of curcumin in a dose-dependent manner. Macroscopic observations were confirmed by histological examinations. Histological changes including infiltration of immune cells, synovial hyperplasia, cartilage destruction, and bone erosion in the hind paw sections were extensively suppressed by curcumin. The histological scores were consistent with clinical arthritis indexes. Production of MMP-1 and MMP-3 were inhibited by curcumin in CIA hind paw sections and tumor necrosis factor (TNF)-α–stimulated FLS and chondrocytes in a dose-dependent manner. As for the mechanism, curcumin inhibited activating phosphorylation of protein kinase Cδ (PKCδ) in CIA, FLS, and chondrocytes. Curcumin also suppressed the JNK and c-Jun activation in those cells. This study suggests that the suppression of MMP-1 and MMP-3 production by curcumin in CIA is mediated through the inhibition of PKCδ and the JNK/c-Jun signaling pathway.</P>