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Baskar Gurunathan,Renganathan Sahadevan 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.1
The sequential optimization strategy for design of an experimental and artificial neural network (ANN)linked genetic algorithm (GA) were applied to evaluate and optimize media component for L-asparaginase production by Aspergillus terreus MTCC 1782 in submerged fermentation. The significant media components identified by Plackett-Burman design (PBD) were fitted into a second order polynomial model (R^2 = 0.910) and optimized for maximum L-asparaginase production using a five-level central composite design (CCD). A nonlinear model describing the effect of variables on L-asparaginase production was developed (R^2 = 0.995) and optimized by a back propagation NN linked GA. Ground nut oil cake (GNOC)flour 3.99% (w/v), sodium nitrate (NaNO_3) 1.04%, Lasparagine 1.84%, and sucrose 0.64% were found to be the optimum concentration with a maximum predicted Lasparaginase activity of 36.64 IU/mL using a back propagation NN linked GA. The experimental activity of 36.97IU/mL obtained using the optimum concentration of media components is close to the predicted L-asparaginase activity of the ANN linked GA.
Neuroprotective Mechanism of Corydaline in Glutamate-Induced Neurotoxicity in HT22 Cells
Baskar Selvaraj,Dae Won Kim,Ki Yeon Yoo,Keunwan Park,Thi Thu Thuy Tran,Jae Wook Lee,Heesu Lee 대한구강생물학회 2024 International Journal of Oral Biology Vol.49 No.1
Glutamate-mediated oxidative stress causes neuronal cell death by increasing intracellular Ca 2+ uptake, reactive oxidative species (ROS) generation, mitogen-activated protein kinase (MAPK) activation, and translocation of apoptosis-inducing factor (AIF) to the nucleus. In the current study, we demonstrated that corydaline exerts potent neuroprotective effects against glutamate-induced neurotoxicity. Treatment with 5 mmol/L glutamate increased cellular Ca 2+ influx, ROS generation, MAPK activation, and AIF translocation. In contrast, corydaline treatment decreased cellular Ca 2+ influx and ROS generation. Western blot analysis revealed that glutamate-mediated MAPK activation was attenuated by corydaline treatment. We further demonstrated that corydaline treatment inhibited the glutamate-mediated translocation of AIF to the nucleus. We propose that corydaline is a promising lead structure for the development of safe and effective neuroprotectants.
Baskar, G,George, Garrick Bikku Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.9
Drugs processed using nanobiotechnology may be more biocompatible, with sustainable and stabilised release or action. L-asparaginase produced from fungi has many advantages for treatment of lymphocytic leukemia with lesser side effect. In the present work, maghemite nanobiocomposites of fungal asparaginase were produced using glutaraldehyde-pretreated colloidal magnetic nanoparticles. Formation of nanobiocomposites was observed using laser light scattering and confirmed by UV-visible spectrophotometry with the absorption peak at 497 nm. The specific asparaginase activity was increased from 320 U/mg with crude asparaginase to 481.5 U/mg. FTIR analysis confirmed that primary amines are the functional groups involved in binding of asparaginase on magnetic nanoparticles. The average size of the produced nanobiocomposite was found in the range of 30 nm to 40 nm using histogram analysis. The magnetic nanobiocomposite of asparaginase synthesised using glutaraldehyde showed 90.75% cytotoxicity against human colon adenocarcinoma cell lines. Hence it can be used as an active anticancer drug with an augmented level of bioavailability.
Engineering glucosinolates in plants: current knowledge and potential uses.
Baskar, Venkidasamy,Gururani, Mayank Anand,Yu, Jae Woong,Park, Se Won Humana Press 2012 Applied biochemistry and biotechnology Vol.168 No.6
<P>Glucosinolates (GSL) and their derivatives are well known for the characteristic roles they play in plant defense as signaling molecules and as bioactive compounds for human health. More than 130 GSLs have been reported so far, and most of them belong to the Brassicaceae family. Several enzymes and transcription factors involved in the GSL biosynthesis have been studied in the model plant, Arabidopsis, and in a few other Brassica crop species. Recent studies in GSL research have defined the regulation, distribution, and degradation of GSL biosynthetic pathways; however, the underlying mechanism behind transportation of GSLs in plants is still largely unknown. This review highlights the recent advances in the metabolic engineering of GSLs in plants and discusses their potential applications.</P>
Synthesis and evaluation of cardamonin derivatives as antiproliferative agents to human cancer cells
Selvaraj Baskar,이상혁,Sang Nguyen Qui Ngoc,이희수,이재욱 대한화학회 2023 Bulletin of the Korean Chemical Society Vol.44 No.3
Twenty novel cardamonin derivatives were synthesized and evaluated for antiproliferative activity against four cancer cell lines (HT-29, DLD-1, MDA-MB-231, and HepG2). Among the derivatives, SWA2 showed the most potent effects with an IC50 value of 4.43–11.0 μM against three cancer cell lines except for HepG2. Further investigation showed that SWA2 induced cancer cell death by apoptosis. Flow cytometry analysis showed that SWA2 treatment increased the ratio of apoptotic and dead cells. Further western blot analysis revealed that SWA2 treatment increased cleaved PARP. In summary, SWA2 can be utilized as a lead structure for anticancer therapy.
Senthilkumar, Baskar,Khan, Ziyauddin,Park, Seungyoung,Kim, Kyoungho,Ko, Hyunhyub,Kim, Youngsik The Royal Society of Chemistry 2015 Journal of Materials Chemistry A Vol.3 No.43
<▼1><P>A high energy hybrid capacitor fabricated from highly porous graphitic carbon and novel electrode material Ni2P2O7 delivers a maximum energy density of 65 W h kg<SUP>−1</SUP> at a power density of 800 W kg<SUP>−1</SUP>, good rate capability and cycling stability in an aqueous Na-ion based electrolyte.</P></▼1><▼2><P>An aqueous Na-ion based hybrid capacitor has been successfully developed by using highly porous graphitic carbon (HPGC) derived from waste writing paper and a new electrode material as a negative and positive electrode, respectively. HPGC was prepared <I>via</I> hydrothermal carbonization and subsequent KOH activation of waste writing paper which showed a highly porous stacked sheet-like morphology with an exceptionally high BET specific surface area (1254 m<SUP>2</SUP> g<SUP>−1</SUP>). HPGC exhibited typical electrical double layer capacitor (EDLC) behavior with a high specific capacitance of 384 F g<SUP>−1</SUP> and good negative working potential (−1.0 V) in an aqueous electrolyte. On the other hand, Ni2P2O7 was synthesized by a simple co-precipitation technique and tested as a cathode material which delivered a maximum specific capacitance of 1893 F g<SUP>−1</SUP> at 2 A g<SUP>−1</SUP> current density. The fabricated HPGC‖Ni2P2O7 hybrid device displayed excellent cyclic stability up to 2000 cycles and delivered a maximum energy density of 65 W h kg<SUP>−1</SUP> at 800 W kg<SUP>−1</SUP> power density in a Na-ion based aqueous electrolyte.</P></▼2>
( Gurunathan Baskar ),( Renganathan Sahadevan ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.7
Optimization of culture conditions for L-asparaginase production by submerged fermentation of Aspergillus terreus MTCC 1782 was studied using a 3-level central composite design of response surface methodology and artificial neural network linked genetic algorithm. The artificial neural network linked genetic algorithm was found to be more efficient than response surface methodology. The experimental L-asparaginase activity of 43.29 IU/ml was obtained at the optimum culture conditions of temperature 35oC, initial pH 6.3, inoculum size 1% (v/v), agitation rate 140 rpm, and incubation time 58.5 h of the artificial neural network linked genetic algorithm, which was close to the predicted activity of 44.38 IU/ml. Characteristics of L-asparaginase production by A. terreus MTCC 1782 were studied in a 3 L bench-scale bioreactor.
Effects of Heat Shock Treatment on Enzymatic Proteolysis for LC-MS/MS Quantitative Proteome Analysis
( Albert Baskar Arul ),( Na Young Han ),( Young Su Jang ),( Hyojin Kim ),( Hwan Mook Kim ),( Hookeun Lee ) 한국질량분석학회 2016 Mass spectrometry letters Vol.7 No.1
Various efforts have been developed to improve sample preparation steps, which strongly depend on hands-on processes for accurate and sensitive quantitative proteome analysis. In this study, we carried out heating the sample prior to trypsin digestion using an instrument to improve the tryptic digestion process. The heat shock generated by the system efficiently denatured proteins in the sample and increased the reproducibility in quantitative proteomics based on peptide abundance measurements. To demonstrate the effectiveness of the protocol, three cell lines (A human lung cancer cell line (A549), a human embryonic kidney cell line (HEK293T), and a human colorectal cancer cell line (HCT-116)) were selected and the effect of heat shock was compared to that of normal tryptic digestion processes. The tryptic digests were desalted and analysed by LC-MS/MS, the results showed 57 and 36% increase in the number of identified unique peptides and proteins, respectively, than conventional digestion. Heat shock treated samples showed higher numbers of shorter peptides and peptides with low inter-sample variation among triplicate runs. Quantitative LC-MS/MS analysis of heat shock treated sample yielded peptides with smaller relative error percentage for the triplicate run when the peak areas were compared. Exposure of heat-shock to proteomic samples prior to proteolysis in conventional digestion process can increase the digestion efficiency of trypsin resulting in production of increased number of peptides eventually leading to higher proteome coverage.
An Automated High Throughput Proteolysis and Desalting Platform for Quantitative Proteomic Analysis
( Albert Baskar Arul ),( Na Young Han ),( Hookeun Lee ) 한국질량분석학회 2013 Mass spectrometry letters Vol.4 No.2
Proteomics for biomarker validation needs high throughput instrumentation to analyze huge set of clinical samples for quantitative and reproducible analysis at a minimum time without manual experimental errors. Sample preparation, a vital step in proteomics plays a major role in identification and quantification of proteins from biological samples. Tryptic digestion a major check point in sample preparation for mass spectrometry based proteomics needs to be more accurate with rapid processing time. The present study focuses on establishing a high throughput automated online system for proteolytic digestion and desalting of proteins from biological samples quantitatively and qualitatively in a reproducible manner. The present study compares online protein digestion and desalting of BSA with conventional off-line (in-solution) method and validated for real time sample for reproducibility. Proteins were identified using SEQUEST data base search engine and the data were quantified using IDEALQ software. The present study shows that the online system capable of handling high throughput samples in 96 well formats carries out protein digestion and peptide desalting efficiently in a reproducible and quantitative manner. Label free quantification showed clear increase of peptide quantities with increase in concentration with much linearity compared to off line method. Hence we would like to suggest that inclusion of this online system in proteomic pipeline will be effective in quantification of proteins in comparative proteomics were the quantification is really very crucial.