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Lee, John Hwa,Hur, Jin,Stein, Barry D. Elsevier 2008 The veterinary journal Vol.176 No.2
<P><B>Abstract</B></P><P>The aims of this study were: (1) to examine whether or not enterohemorrhagic <I>Escherichia coli</I> O26 and O111 (EHEC O26 and O111) are involved in neonatal calf diarrhea; (2) to determine the specific age periods at which the calves are vulnerable to these organisms, and (3) to reveal the biochemical, genetic and cytotoxic characteristics of the isolates. The study investigated the occurrence of EHEC O26 and O111 in calves associated with or without diarrhea. A total of 442 diarrheic and non-diarrheic young calves from 115 different farms were examined. Of the 257 calves with diarrhea, 37 (14.4%) and 32 (12.5%) tested positive for EHEC O26 and EHEC O111, respectively. Of the 185 non-diarrheic calves, 14 (7.6%) and 11 (5.9%) tested positive for EHEC O26 and EHEC O111, respectively. EHEC O26 and O111 were recovered from 14/69 (20%) and 11/69 (16%) diarrheic calves <2-weeks-old, respectively, and no EHEC O26 and O111 were detected in the non-diarrheic claves of this age group, suggesting that EHEC O26 and O111 are possible causes of the disease in infected neonatal calves. However, there were similar rates of occurrence in the diarrheic and non-diarrheic calves in the older animals (particularly, aged >10 weeks). PCR analysis showed that the isolates carried various virulence genes such as <I>Ehly</I>, <I>eae</I>, <I>stx1</I> and <I>stx2</I>, which highlight the potential importance of these attributes for the infection, colonization and the possible pathogenesis of calf diarrhea. Cytotoxicity analysis revealed that many of the EHEC isolates showed high cytotoxicity to Vero cells, re-emphasizing the potential for cattle being a direct source of EHEC infections in humans.</P>
Valentin N. Sapunov,Antonina A. Stepacheva,Esther M. Sulman,Johan Wärnåc,Päivi Mäki-Arvela,Mikhail G. Sulman,Alexander I. Sidorov,Barry D. Stein,Dmitry Yu. Murzin,Valentina G. Matveeva 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.46 No.-
Stearic acid hydrodeoxygenation into n-heptadecane on the catalyst containing Pd nanoparticlesincorporated in the hypercrosslinked polystyrene matrix was studied. The used catalysts werecharacterized using TEM, CO chemisorption, low-temperature nitrogen physisorption and XPS. A clearhydrogen pressure dependent induction period was noticed. Kinetic regularities were studied for theindicated reaction in the presence of polymer based catalysts. A mathematical model for decarboxylationwas suggested which was able to adequately describe experimental data at different temperatures,hydrogen pressures and initial concentrations of stearic acid. Kinetic constants were calculated and theirstatistical analysis was performed.