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AN, JUNGHWA,MIN, MI-SOOK,SOMMER, JULIE,LOUIS JR, EDWARD,BRENNEMAN, RICK,KWON, SOO-WHAN,SHIN, NAM SHIK,LEE, HANG Blackwell Science Ltd 2005 MOLECULAR ECOLOGY NOTES Vol.5 No.2
<P>Abstract</P><P>Microsatellites have been applied in a variety of fields, including conservation genetics. Species-specific microsatellites are considered as more powerful genetic markers to generate an accurate genetic composition of a species itself. Accordingly, we characterized 15 polymorphic microsatellite loci from Korean goral, <I>Nemorhaedus caudatus</I>, one of the most endangered species in South Korea. The new markers should benefit future studies of the endangered species of other Asian gorals and their relatives for the study of genetic diversity and potential conservation management.</P>
안정화,최성경,Julie Sommer,Edward Louis Jr,Rick Brenneman,Barbora Zemanová,Petra Hájková,Grimm Park,Mi-Sook Min,김경석,이항 대한수의학회 2010 Journal of Veterinary Science Vol.11 No.4
In order to screen microsatellites for conservation genetics studies of the species, a total of 23 microsatellite loci from Korean goral (Naemorhedus caudatus), including 15 previously developed loci and 8 new loci in this study, were tested. Eleven microsatellites were screened and subjected to cross-species amplification using a test panel of four Caprinae species, Japanese serows (Capricornis crispus), Chinese gorals (Naemorhedus goral), Northern chamois (Rupicapra rupicapra) and domestic goats (Capra hircus). In addition, all eleven microsatellites (SY3A, SY12A, SY12B, SY48, SY58, SY71, SY76, SY84, SY84B, SY112, and SY129) satisfied the criteria to be a core set of microsatellites. This core set of microsatellites and cross-species amplification of Korean goral microsatellites were found to be helpful for high-resolution studies for conservation and management of Korean goral and other endangered Caprinae species.
Resolving the evolution of extant and extinct ruminants with high-throughput phylogenomics.
Decker, Jared E,Pires, J Chris,Conant, Gavin C,McKay, Stephanie D,Heaton, Michael P,Chen, Kefei,Cooper, Alan,Vilkki, Johanna,Seabury, Christopher M,Caetano, Alexandre R,Johnson, Gary S,Brenneman, Rick National Academy of Sciences 2009 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.106 No.44
<P>The Pecorans (higher ruminants) are believed to have rapidly speciated in the Mid-Eocene, resulting in five distinct extant families: Antilocapridae, Giraffidae, Moschidae, Cervidae, and Bovidae. Due to the rapid radiation, the Pecoran phylogeny has proven difficult to resolve, and 11 of the 15 possible rooted phylogenies describing ancestral relationships among the Antilocapridae, Giraffidae, Cervidae, and Bovidae have each been argued as representations of the true phylogeny. Here we demonstrate that a genome-wide single nucleotide polymorphism (SNP) genotyping platform designed for one species can be used to genotype ancient DNA from an extinct species and DNA from species diverged up to 29 million years ago and that the produced genotypes can be used to resolve the phylogeny for this rapidly radiated infraorder. We used a high-throughput assay with 54,693 SNP loci developed for Bos taurus taurus to rapidly genotype 678 individuals representing 61 Pecoran species. We produced a highly resolved phylogeny for this diverse group based upon 40,843 genome-wide SNP, which is five times as many informative characters as have previously been analyzed. We also establish a method to amplify and screen genomic information from extinct species, and place Bison priscus within the Bovidae. The quality of genotype calls and the placement of samples within a well-supported phylogeny may provide an important test for validating the fidelity and integrity of ancient samples. Finally, we constructed a phylogenomic network to accurately describe the relationships between 48 cattle breeds and facilitate inferences concerning the history of domestication and breed formation.</P>