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Expression of Sara2 Human Gene in Erythroid Progenitors
Jardim, Denis Leonardo Fontes,Cunha, Anderson Ferreira Da,Duarte, Adriana Da Silva Santos,Santos, Camila Oresco Dos,Saad, Sara Terezinha Olalla,Costa, Fernando Ferreira Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.3
A human homologue of Sar1, named Sara2, was shown to be preferentially expressed during erythropoiesis in a culture stimulated by EPO. Previous studies, in yeast, have shown that secretion-associated and Ras-related protein (Sar1p) plays an essential role in protein transport from the endoplasmic reticulum to the Golgi apparatus. Here, we report the molecular analysis of Sara2 in erythroid cell culture. A 1250 bp long cDNA, encoding a 198 amino-acid protein very similar to Sar1 proteins from other organisms, was obtained. Furthermore, we also report a functional study of Sara2 with Real-time quantitative PCR analysis, demonstrating that expression of Sara2 mRNA increases during the initial stages of erythroid differentiation with EPO and that a two-fold increase in expression occurs following the addition of hydroxyurea (HU). In K562 cells, Sara2 mRNA was observed to have a constant expression and the addition of HU also up-regulated the expression in these cells. Our results suggest that Sara2 is an important gene in processes involving proliferation and differentiation and could be valuable for understanding the vesicular transport system during erythropoiesis.
Oxidative stress mediated cytogenotoxicological effects of phytol in wistar albino rats
Marcus Vinícius Oliveira Barros de Alencar,Muhammad Torequl Islam,Antonielly Campinho dos Reis,Santos José Victor de Oliveira,Adriana Maria Viana Nunes,Felipe Cavalcante Carneiro da Silva,Machado Keyl 경희대학교 융합한의과학연구소 2023 Oriental Pharmacy and Experimental Medicine Vol.23 No.1
Phytol (PHY), a diterpenoid, is known for its various bio-pharmacological activities. However, its toxicological profile has yet to be evaluated. The aim of this study was to evaluate the cytogenotoxicological profile of PHY in Wistar albino rats. Forty-five female non-pregnant rats were treated acutely and subchronically with PHY at doses of 300 and 2000 mg/kg and 30, 60 and 90 mg/kg for 14 and 28 days. Neuropharmacological, genotoxic, and mutagenic effects were investigated. The results suggest that PHY did not cause the death of rats at a dose of 2000 mg/kg, suggesting a higher range of the LD50 of this diterpenoid. Several toxicological alterations were observed in clinical and neuropharmacological parameters depending upon the doses. No hepatic histopathological changes were observed. PHY induced genotoxicity in peripheral blood, bone marrow, liver, and kidney. PHY did not show damage repair activity in peripheral blood lymphocytes. In the bone marrow, both acute and subchronic PHY treatments increased micronucleus frequency, indicating a mutagenic effect. PHY caused neuropharmacological alterations and genetic instability, possibly through the oxidative stress induction pathway.